Abstract:
:Irreversible conversion of the purified zinc-containing 7Fe ferredoxin from the thermoacidophilic archaeon Sulfolobus sp. strain 7 was found to occur under aerobic conditions at pH 5.0 and at 4 degrees C. This process accompanied a substantial increase of the electron paramagnetic resonance signal attributed to a [3Fe-4S]1+ cluster, and the converted form containing approximately 6 Fe/Zn (mol/mol) had a net charge different from that of the native 7Fe ferredoxin. These data provide evidence for the formation of a stable intermediate product of the archaeal ferredoxin having two [3Fe-4S] clusters and a zinc center by artificial oxidative degradation. This further explains the discrepancy that a zinc center and two [3Fe-4S] clusters (but not a zinc center and one [3Fe-4S] cluster plus one [4Fe-4S] cluster) are observed in the crystal structure at pH 5.0.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Iwasaki T,Oshima Tdoi
10.1016/s0014-5793(97)01286-6subject
Has Abstractpub_date
1997-11-10 00:00:00pages
223-6issue
2eissn
0014-5793issn
1873-3468pii
S0014-5793(97)01286-6journal_volume
417pub_type
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