A C-terminal proline is required for bioluminescence of the Ca(2+)-binding photoprotein, aequorin.

abstract：:Novel catechin derivatives obtained from grape procyanidins and l-cysteine scavenge free radicals by hydrogen atom donation, rather than electron transfer, and reduce cell viability in A375 and M21 melanoma cells. In particular, 4beta-(S-cysteinyl)epicatechin 3-O-gallate has a free radical scavenging capacity as stron...

journal_title：FEBS letters

authors： Lozano C,Torres JL,Julià L,Jimenez A,Centelles JJ,Cascante M

更新日期：2005-08-15 00:00:00

abstract：:The purpose of the present study was to investigate whether 5-hydroxytryptamine (5-HT, serotonin) affects migration of vascular endothelial cells. 5-HT significantly enhanced migration of human aortic endothelial cells (HAECs), and this enhancement was completely inhibited by GR 55562, a 5-HT1 receptor antagonist, and...

journal_title：FEBS letters

authors： Matsusaka S,Wakabayashi I

更新日期：2005-12-19 00:00:00

abstract：:Highly active adenylylsulfate (APS) reductase was isolated under N(2)/H(2) from sulfate-reducing and sulfide-oxidizing bacteria and archaea. It was a 1:1 alphabeta-heterodimer of molecular mass approximately 95 kDa, and two subunits (alpha approximately 75, beta approximately 20 kDa). The specific activity was 11-14 m...

journal_title：FEBS letters

authors： Fritz G,Büchert T,Huber H,Stetter KO,Kroneck PM

更新日期：2000-05-04 00:00:00

abstract：:The ubiquitin-editing enzyme A20 suppresses nuclear factor-κB (NF-κB) activation and tumor necrosis factor-α (TNF-α)-induced apoptosis in a deubiquitinating and ubiquitin ligase activity-dependent manner. Although recent studies revealed that A20 regulates NF-κB independently of its enzymatic activity through its seve...

journal_title：FEBS letters

authors： Yamaguchi N,Yamaguchi N

更新日期：2015-05-22 00:00:00

abstract：:The presence of synaptic proteins involved in excitation/secretion coupling was examined in ten small cell lung cancer lines. N-Type calcium channels (omega-conotoxin receptors), synaptotagmin (p65) and syntaxin (HPC-1) were detected in eight. Co-immunoprecipitation experiments indicated that syntaxin can form a compl...

journal_title：FEBS letters

authors： David P,el Far O,Martin-Mouto N,Poupon MF,Takahashi M,Seagar MJ

更新日期：1993-07-12 00:00:00

abstract：:Differentiation of glia (astrocytes and oligodendrocytes) in Drosophila requires the gene glial cells missing (gcm), which controls lineage determination. In the absence of gcm, neuroglia progenitors exclusively differentiate into neurons, instead of into both neurons and glia. In contrast, ectopic overexpression of g...

journal_title：FEBS letters

authors： Altshuller Y,Copeland NG,Gilbert DJ,Jenkins NA,Frohman MA

更新日期：1996-09-16 00:00:00

abstract：:The phosphatidylinositol 3-kinase/Akt pathway is responsible for key aspects of tumor progression, and is frequently hyperactivated in cancer. We have recently identified palladin, an actin-bundling protein that functions to control the actin cytoskeleton, as an Akt1-specific substrate that inhibits breast cancer cell...

journal_title：FEBS letters

authors： Chin YR,Toker A

更新日期：2010-12-01 00:00:00

abstract：:The sarcoplasmic reticulum-bound creatine kinase from rabbit skeletal muscle was inhibited by the nitric oxide donor S-nitrosoglutathione (GSNO). This led to a decrease in Ca2+ uptake in sarcoplasmic reticulum vesicles when the transport was driven by ATP generated from phosphocreatine and ADP. In contrast, the Ca 2+ ...

journal_title：FEBS letters

authors： Wolosker H,Panizzutti R,Engelender S

更新日期：1996-09-02 00:00:00

abstract：:A full-length cDNA coding for troponin-C isolated from an adult rabbit fast skeletal muscle library has been sequenced and expressed in an E. coli host. The amino acid sequence derived from the coding region agrees with the published protein sequence except that the first two residues are reversed. The expressed prote...

journal_title：FEBS letters

authors： Chen Q,Taljanidisz J,Sarkar S,Tao T,Gergely J

更新日期：1988-02-08 00:00:00

abstract：:Farnesoic acid is a signaling molecule that inhibits the transition from budding yeast to filament formation in Candida albicans, but the molecular mechanism regulated by this substance is unknown. In this study, we analyzed the function of CaPHO81, which is induced by farnesoic acid. The pho81Δ mutant cells existed e...

journal_title：FEBS letters

authors： Chung SC,Kim TI,Ahn CH,Shin J,Oh KB

更新日期：2010-11-19 00:00:00

abstract：:Domain structures of porcine lung and testicular angiotensin-converting enzymes (ACE) were studied by radiation inactivation to test the hypothesis that lung ACE has two catalytic sites localized to discrete, structurally independent domains (the N- and C-domains) of approximately equal size. The minimum functional si...

journal_title：FEBS letters

authors： Sakaguchi H,Hirose S,Kume T,Hagiwara H

更新日期：1992-06-29 00:00:00

abstract：:A large number of protein-protein interactions involved turn or loop regions. The excised linear peptides from these regions reveal complex conformational averaging. To circumvent this motional averaging and to stabilize the beta-turn conformation, extensive effort has been devoted to the design of constrained peptido...

journal_title：FEBS letters

authors： Lee MS,Gardner B,Kahn M,Nakanishi H

更新日期：1995-02-13 00:00:00

abstract：:1,3-alpha-D-Glucan synthase of Streptococcus mutans catalyzes both the hydrolysis of sucrose to glucose and fructose, and the glucosyl transfer to glucosyl polymers to yield water-insoluble glucan. The enzyme catalyzes only sucrose hydrolysis, however, in the absence of 1,6-alpha-D-glucan as an acceptor. In the presen...

journal_title：FEBS letters

authors： Yamashita Y,Hanada N,Itoh-Andoh M,Takehara T

更新日期：1989-01-30 00:00:00

abstract：:Carboxylesterase (EII') from Arthrobacter sp. KI72 has 88% homology to 6-aminohexanoate-dimer hydrolase (EII) and possesses ca. 0.5% of the level of 6-aminohexanoate-linear dimer (Ald)-hydrolytic activity of EII. To study relationship between Ald-hydrolytic and esterolytic activities, random mutations were introduced ...

journal_title：FEBS letters

authors： Ohki T,Wakitani Y,Takeo M,Yasuhira K,Shibata N,Higuchi Y,Negoro S

更新日期：2006-09-18 00:00:00

abstract：:The human pathogen Vibrio vulnificus undergoes phase variation among colonial morphotypes, including a virulent opaque form which produces capsular polysaccharide (CPS) and a translucent phenotype that produces little or no CPS and is attenuated. Here, we found that a V. vulnificus mutant defective for RfaH antitermin...

journal_title：FEBS letters

authors： Garrett SB,Garrison-Schilling KL,Cooke JT,Pettis GS

更新日期：2016-12-01 00:00:00

abstract：:The accumulation of pre-PhoS under conditions of PhoS overproduction has been previously described. It is now demonstrated that during the induction of PhoS, a delay in the completion of polypeptide chain elongation can be detected. This delay is related to the extent of jamming of export sites by pre-PhoS or by other...

journal_title：FEBS letters

authors： Anba J,Lazdunski C,Pages JM

更新日期：1986-02-03 00:00:00

abstract：:The bacterial twin-arginine translocation (Tat) pathway is distinct from the Sec system by its remarkable capacity to export folded enzymes. To address the question whether the two systems are capable of translocating homologous enzymes catalyzing the same reaction, we cloned the tap gene encoding Thermus thermophilus...

journal_title：FEBS letters

authors： Angelini S,Moreno R,Gouffi K,Santini C,Yamagishi A,Berenguer J,Wu L

更新日期：2001-10-05 00:00:00

abstract：:A cDNA of a type 7 adenylyl cyclase isoform was cloned from a bovine retinal pigment epithelium cDNA library using oligonucleotides developed to conserved regions common to mammalian adenylyl cyclases. A 6.7 kb mRNA of very high abundance was uniquely present on Northern blots containing mRNA or total RNA from the pig...

journal_title：FEBS letters

authors： Völkel H,Beitz E,Klumpp S,Schultz JE

更新日期：1996-01-15 00:00:00

abstract：:Tyrosine phosphorylation of Cbl and its association with signal-transducing molecules in response to macrophage colony-stimulating factor (M-CSF) were analyzed by using cell lines which express the wild-type and a mutant M-CSF receptor, Fms. We found that in a clone, F723 TF-1 cells expressing mutant Fms in which tyro...

journal_title：FEBS letters

authors： Ota J,Sato K,Kimura F,Wakimoto N,Nakamura Y,Nagata N,Suzu S,Yamada M,Shimamura S,Motoyoshi K

更新日期：2000-01-21 00:00:00

abstract：:Cathelicidins are a group of myeloid antimicrobial peptide precursors found in a variety of mammalian species. Transcripts of this family show a highly conserved 5' region corresponding to the 5' untranslated region, signal peptide and propiece, and diverse 3' regions encoding structurally varied C-terminal sequences ...

journal_title：FEBS letters

authors： Scocchi M,Wang S,Zanetti M

更新日期：1997-11-17 00:00:00

abstract：:Several types of post-translational modifications contribute to the high level of tubulin heterogeneity in the brain. An important modification is glutamylation of the major brain-specific isotypes, such as class Ia/b of alpha-tubulin and classes II and III of beta-tubulin. Here we describe experiments to determine if...

journal_title：FEBS letters

authors： Mary J,Redeker V,Le Caer JP,Promé JC,Rossier J

更新日期：1994-10-10 00:00:00

abstract：:Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be dis...

journal_title：FEBS letters

authors： Laukens K,Lenobel R,Strnad M,Van Onckelen H,Witters E

更新日期：2003-01-02 00:00:00

abstract：:Major myelin proteolipid (MMPL, also called PLP) and DM-20 are the two major intrinsic membrane proteins of CNS myelin. A specific immunological probe was obtained for MMPL by raising antibodies against the synthetic tridecapeptide 117-129 of MMPL. Antibodies against this peptide reacted with the MMPL but did not cros...

journal_title：FEBS letters

authors： Trifilieff E,Luu B,Nussbaum JL,Roussel G,Espinosa de los Monteros A,Sabatier JM,Van Rietschoten J

更新日期：1986-03-31 00:00:00

abstract：:Gap junctions (GJs) traverse apposing membranes of neighboring cells to mediate intercellular communication by passive diffusion of signaling molecules. We have shown previously that cells endocytose GJs utilizing the clathrin machinery. Endocytosis generates cytoplasmic double-membrane vesicles termed annular gap jun...

journal_title：FEBS letters

authors： Fong JT,Nimlamool W,Falk MM

更新日期：2014-03-03 00:00:00

abstract：:MeCP2 is a chromatin-associated protein that is mutated in Rett syndrome. Its methyl-CpG-binding domain interacts with DNA containing methylated cytosine, but other modes of recruitment to the genome have also been proposed. Here, we use in vitro and in vivo assays to investigate the DNA binding specificity of two AT-...

journal_title：FEBS letters

authors： Lyst MJ,Connelly J,Merusi C,Bird A

更新日期：2016-09-01 00:00:00

abstract：:An essential difference between eukaryotic ferritins and bacterioferritins is that the latter contain naturally, in vivo haem as Fe-protoporphyrin IX. This haem is located in a hydrophobic pocket along the 2-fold symmetry axes and is liganded by two Met 52. However, in in vivo studies, a cofactor has been isolated in ...

journal_title：FEBS letters

authors： de Val N,Herschbach H,Potier N,Dorsselaer AV,Crichton RR

更新日期：2006-11-13 00:00:00

abstract：:Several blood-feeding organisms, including the malaria parasite detoxify haem released from host haemoglobin by conversion to the insoluble crystalline ferriprotoporphyrin IX dimer known as haemozoin. To date the mechanism of haemozoin formation has remained unknown, although lipids or proteins have been suggested to ...

journal_title：FEBS letters

authors： Egan TJ,Chen JY,de Villiers KA,Mabotha TE,Naidoo KJ,Ncokazi KK,Langford SJ,McNaughton D,Pandiancherri S,Wood BR

更新日期：2006-09-18 00:00:00

abstract：:Members of the connexin family differ most in their carboxy-termini, both with respect to sequence and length. In order to assess the contribution of this region to channel function, a series of carboxy-terminal deletion mutants were tested in the paired-oocyte expression system. Connexin-32 can be truncated by 64 ami...

journal_title：FEBS letters

authors： Rabadan-Diehl C,Dahl G,Werner R

更新日期：1994-08-29 00:00:00

abstract：:The control of physiological processes requires the regulation and coordination of many different signals and is determined in part by the activation and repression of expression of specific target genes. RIP140 is a ligand dependent coregulator of many nuclear receptors that influence such diverse processes as muscle...

journal_title：FEBS letters

authors： White R,Morganstein D,Christian M,Seth A,Herzog B,Parker MG

更新日期：2008-01-09 00:00:00

abstract：:The dgd1 mutant of Arabidopsis thaliana provides us with a powerful tool for revealing the specific role of digalactosyldiacylglycerol (DGDG) in photosynthesis. Blue-native polyacrylamide gel electrophoresis analysis revealed that photosystem I (PSI) subunits are assembled into a PSI complex, and that a PSI subcomplex...

journal_title：FEBS letters

authors： Guo J,Zhang Z,Bi Y,Yang W,Xu Y,Zhang L

更新日期：2005-07-04 00:00:00