Abstract:
:Like glutathione or dithiothreitol, metallothionein effects the formation of pancreatic ribonuclease A from its S-sulfonated derivative catalyzed by protein disulfide isomerase. EDTA increases the yield of ribonuclease A activity recovery with metallothionein but does not affect the reaction with glutathione or dithiothreitol. EDTA also increases the reactivity of thiol groups in metallothionein with 5,5'-dithiobis-(2-nitrobenzoic acid) by chelation of zinc ions. It is suggested that the thiol groups in metallothionein form a part of the pool of cellular thiols in the regulation of cellular redox reactions and their availability is modulated by zinc chelation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Zhang S,Li J,Wang CC,Tsou CLdoi
10.1016/s0014-5793(99)01562-8keywords:
subject
Has Abstractpub_date
1999-12-03 00:00:00pages
383-6issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(99)01562-8journal_volume
462pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Antithrombin Glasgow is a hereditary abnormal antithrombin that has lost thrombin inhibitory activity. It was isolated from the plasma of a 41-year-old male with a history of thrombotic events. Antithrombin Glasgow was purified from plasma using heparin-Sepharose chromatography at pH 7.4 eluting with increasing concen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80841-x
更新日期:1988-04-25 00:00:00
abstract::Histone acetyltransferase activity was measured in isolated nuclei during the synchronous cell cycle of the myxomycete Physarum polycephalum. Nuclei were incubated with [14C]acetyl-coenzyme A and an excess of exogenous calf thymus histones. The activity is periodic during the cell cycle; it rises during the S-phase to...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80394-0
更新日期:1987-10-05 00:00:00
abstract::Pi binding by the F(1)-ATPase of beef heart mitochondria and of the Escherichia coli plasma membrane (E. coli F(1)) was examined by two methods: the centrifuge column procedure [Penefsky, H.S. (1977) J. Biol. Chem. 252, 2891-2899] and the Paulus pressure dialysis cell [Paulus, H. (1969) Anal. Biochem. 32, 91-100]. The...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.02.072
更新日期:2005-04-11 00:00:00
abstract::The early response gene IEX-1 modulates apoptosis and cell growth in a poorly defined fashion. Here, we describe the effect of hammerhead ribozymes specifically disrupting IEX-1 expression in 293 cells. Compared to vector control, 293 cells exhibit a reduced growth rate and a slowed cell cycle progression, when stably...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02344-4
更新日期:2001-04-13 00:00:00
abstract::Lysophosphatidic acid (LPA) exerts multiple biological functions through G protein-coupled receptors (EDG2/LPA(1), EDG4/LPA(2), and EDG7/LPA(3)) and is present in serum where it is associated with albumin. In this study we examined LPA activity in various biological fluids by measuring the LPA-induced increase in the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02976-9
更新日期:2002-07-17 00:00:00
abstract::Organelles have been studied traditionally as single units, but a novel concept is now emerging: each organelle has distinct functional zones that regulate specific functions. The Golgi apparatus seems to have various zones, including zones for: glycosylphosphatidylinositol-anchored proteins; proteoglycan, mucin and l...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.13554
更新日期:2019-09-01 00:00:00
abstract::Stroke is characterized by massive inflammation in areas surrounding the injury that magnifies damage to the brain. The liver X receptors (LXRs) are nuclear receptors that regulate cholesterol, lipid, and glucose metabolism. Synthetic LXR agonists have potent anti-inflammatory properties in a variety of settings, incl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.08.035
更新日期:2008-10-15 00:00:00
abstract::The influence of duplex stabilizing oligonucleotide-effector (oligonucleotide, carrying N-(2-hydroxyethyl)phenazinium residues on both ends), on selectivity of site-directed modification of E. coli 16 S rRNA (1542 nucleotides in length) under the conditions of its secondary structure stability was studied. The constan...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81110-a
更新日期:1990-08-20 00:00:00
abstract::Dun1p and Rad53p of the budding yeast Saccharomyces cerevisiae are members of a conserved family of cell cycle checkpoint protein kinases that contain forkhead-associated (FHA) domains. Here, we demonstrate that these FHA domains contain 130-140 residues, and are thus considerably larger than previously predicted by s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01392-2
更新日期:2000-04-14 00:00:00
abstract::The profiling of grapevine (Vitis vinifera L.) genes under water deficit was specifically targeted to sugar transporters. Leaf water status was characterized by physiological parameters and soluble sugars content. The expression analysis provided evidence that VvHT1 hexose transporter gene was strongly down-regulated ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.09.016
更新日期:2014-11-03 00:00:00
abstract::We have expressed the cloned rat kappa-opioid receptor in human embryonic kidney 293 cells and studied the ability of kappa-selective ligands to inhibit adenylyl cyclase. In transfected 293 cells, activation of the kappa-opioid receptor by U50,488 and the dynorphins resulted in the inhibition of cAMP accumulation. The...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00088-q
更新日期:1995-02-20 00:00:00
abstract::IRF-8/ICSBP and IRF-1 are IRF family members whose expression is induced in response to IFN-gamma in macrophages. IL-12 is a cytokine produced in macrophages that plays a critical role in host defense. IFN-gamma and bacterial lipopolysaccharide (LPS) induce IL-12p40 transcription, which is necessary for the production...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03556-1
更新日期:2002-11-06 00:00:00
abstract::The four chain agglutinins from Abrus precatorius, Viscum album and Ricinus communis promote depurination of the 28 S rRNA from rabbit reticulocyte ribosomes characteristic of the common ribosome-inactivating proteins (RIPs). These agglutinins inhibited mammalian protein synthesis at nanomolar concentrations but they ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80193-x
更新日期:1993-08-23 00:00:00
abstract::Treatment of U937 cells with dolichyl phosphate led to an increase in the activity of the ICE family protease CPP32, accompanied with cleavage of pre-CPP32 to generate p17. Peptide inhibitors YVAD-cmk and Z-Asp-CH2-DCB (specific to ICE) and DEVD-CHO (specific to CPP32) blocked the dolichyl phosphate-induced apoptosis....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00763-1
更新日期:1997-07-21 00:00:00
abstract::CCN2 plays a critical role in the development of mesenchymal tissues such as cartilage and bone, and the binding of CCN2 to various cytokines and receptors regulates their signaling.By screening a protein array, we found that CCN2 could bind to fibroblast growth factor receptors (FGFRs) 2 and 3, with a higher affinity...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.10.038
更新日期:2012-12-14 00:00:00
abstract::Cellular memory is a ubiquitous phenomenon in cell biology. Using numerical simulation and theoretical analysis, we explored the robustness of cellular memory to intrinsic noise in a transcriptional positive feedback system. Without noise, the system could create two stable steady states and function as a memory modul...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.10.005
更新日期:2008-11-12 00:00:00
abstract::FoxM1 (previously named WIN, HFH-11 or Trident) is a Forkhead box (Fox) transcription factor widely expressed in proliferating cells. Various findings, including a recent analysis of FoxM1 knockout mice, suggest that FoxM1 is required for normal S-M coupling during cell cycle progression. To study the regulatory role ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02915-5
更新日期:2001-10-19 00:00:00
abstract::Degradation of extracellular matrix proteins is performed by metalloproteinases which are inhibited by tissue inhibitors of metalloproteinases (TIMP). We expressed the murine TIMP-1 protein in E. coli and prepared a polyclonal antiserum against the recombinant protein. Using this antiserum we studied the biosynthesis ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00636-9
更新日期:1994-07-25 00:00:00
abstract::Transport of the precursor for carbamoyl-phosphate synthetase I into mitochondria in Reuber hepatoma H-35 cells was inhibited by adding monensin or nigericin to the culture medium at a concentration of 0.5 microM, and the enzyme precursor accumulated, mainly in the cytosolic fraction. Accumulated precursor was degrade...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80029-0
更新日期:1984-01-02 00:00:00
abstract::The pathogenicities of influenza viruses and paramyxoviruses have been proposed to be primarily determined by a host cell protease(s) that activates viral infectivity by proteolytic cleavage of the envelope glycoproteins. We recently isolated a trypsin-type endoprotease, named tryptase Clara, from rat bronchial and br...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81549-f
更新日期:1993-05-10 00:00:00
abstract::When acquiring internal membranes and vesicular transport, eukaryotic cells started to synthesize sphingolipids and sterols. The physical differences between these and the glycerophospholipids must have enabled the cells to segregate lipids in the membrane plane. Localizing this event to the Golgi then allowed them to...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2009.10.020
更新日期:2010-05-03 00:00:00
abstract::Red fluorescent proteins (RFPs) combined with GFP are attractive probes for double-fluorescence labeling of proteins in live cells. However, the application of these proteins is restrained by stable oligomer formation and by their weak fluorescence in vivo. Previous attempts to eliminate these problems by mutagenesis ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.09.084
更新日期:2004-11-05 00:00:00
abstract::A role for the cytokines produced by tissue-infiltrated inflammatory cells (mainly T-lymphocytes and mast cells) in the pathophysiology of fibrosis has been suggested by several groups. Among the products of these cells, interleukin-4 (IL-4) might be one of the factors involved in the initiation of the fibrotic proces...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80448-p
更新日期:1992-05-18 00:00:00
abstract::Nitric oxide, as well as being a major regulator of vascular reactivity, has been shown to be one of the mediators of cytotoxicity in macrophages. This cytotoxic effect seems to be due to the interaction between nitric oxide and oxygen-related free radicals. This study shows that, in vitro, nitric oxide reacts with hy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80621-z
更新日期:1993-04-19 00:00:00
abstract::The regulatory system of smooth muscle thin filaments is thought to involve a major calcium-calmodulin and actin binding protein: caldesmon. A dissective approach was used to isolate a 35 kDa C-terminal fragment of the molecule and to produce antibodies reacting against both the intact and the 15 kDa N-terminal end of...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81150-m
更新日期:1990-08-20 00:00:00
abstract::The addition of 5-azacytidine to erythroleukemic cells which were induced to differentiate with DMSO or BA altered the expression of the hemoglobins. After the addition of 5-azacytidine there was an increase in hemoglobin synthesis especially in the embryonic E2 band. The beta-globin increased in synthesis after 5-aza...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80803-5
更新日期:1983-10-17 00:00:00
abstract::Saccharomyces bayanus var. uvarum investigated here is the species closest to Saccharomyces cerevisiae. Random sequence tags (RSTs) allowed us to identify homologues to 2789 open reading frames (ORFs) in S. cerevisiae, ORFs duplicated in S. uvarum but not in S. cerevisiae, centromeres, tRNAs, homologues of Ty1/2 and T...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02276-6
更新日期:2000-12-22 00:00:00
abstract::The phosphate transporter Pho84 of Saccharomyces cerevisiae is predicted to contain 12 transmembrane (TM) regions, divided into two partially duplicated parts of 6 TM segments. The three-dimensional (3D) organization of the Pho84 protein has not yet been determined. However, the 3D crystal structure of the Escherichia...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.11.012
更新日期:2004-12-17 00:00:00
abstract::Protein synthesis in cell-free systems of rat liver and kidney decreases markedly with age. Examination of activity changes of the different steps revealed for both types of organs that reduced binding of aminoacyl-tRNA to ribosomes and reduced peptidyl transfer might be of major importance for the decrease in overall...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80948-x
更新日期:1983-08-22 00:00:00
abstract::Rapid degradation of specific regulatory proteins plays a role in a wide range of cellular phenomena, including cell cycle progression and the regulation of cell growth and differentiation. A major mechanism of selective protein turnover in vivo involves a large multi-subunit protease known as the proteasome or multi-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01085-4
更新日期:1994-10-31 00:00:00