Abstract:
:The aim of this study was to evaluate the involvement of oxidative stress in glutamate-evoked transglutaminase (TGase) upregulation in astrocyte cultures (14 DIV). A 24 h exposure to glutamate caused a dose-dependent depletion of glutathione intracellular content and increased the ROS production in cell cultures. These effects were receptor-mediated, as demonstrated by inhibition with GYKI 52466. The pre-incubation with glutathione ethyl ester or cysteamine recovered oxidative status and was effective in significantly reducing glutamate-increased tissue TGase. These data suggest that tissue TGase upregulation may be part of a biochemical response to oxidative stress induced by a prolonged exposure of astrocyte cultures to glutamate.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Campisi A,Caccamo D,Li Volti G,Currò M,Parisi G,Avola R,Vanella A,Ientile Rdoi
10.1016/j.febslet.2004.10.074keywords:
subject
Has Abstractpub_date
2004-12-03 00:00:00pages
80-4issue
1-2eissn
0014-5793issn
1873-3468pii
S0014579304013298journal_volume
578pub_type
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