Abstract:
:Changes in troponin C (TnC) conformation upon Ca(2+) binding forms the basis for regulatory and structural functions of TnC molecules. In the present study, Ca(2+)-induced conformational changes in TnC were observed by mechanical measurements. TnC films were prepared by drying or electrospraying TnC solutions, cross-linked with glutaraldehyde, and isometric tension and stiffness measured as a function of pCa. An increase in Ca(2+) from a pCa of 9 to 4 induced large-scale mechanical changes in the TnC films causing several percent shrinkage of the unloaded films. This shrinkage could be partially assigned to Ca(2+) binding to the Ca(2+)/Mg(2+) sites of TnC.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Bukatina AE,Morozov VN,Gusev NB,Sieck GCdoi
10.1016/s0014-5793(02)03013-2keywords:
subject
Has Abstractpub_date
2002-07-31 00:00:00pages
107-10issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579302030132journal_volume
524pub_type
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