Parallel purine-pyrimidine-purine triplex: experimental evidence for existence.

abstract：:Here we present a method to purify large amounts of highly pure and stably arrested ribosome-nascent chain complexes (RNCs) from Escherichia coli cells. It relies on the combined use of translation-arrest sequences to generate nascent polypeptides of specified length and subsequent tag purification of the RNCs. Moreov...

journal_title：FEBS letters

authors： Rutkowska A,Beerbaum M,Rajagopalan N,Fiaux J,Schmieder P,Kramer G,Oschkinat H,Bukau B

更新日期：2009-07-21 00:00:00

abstract：:Phorbol-esters have been shown to modulate the beta-adrenergic-stimulated adenylyl cyclase in a number of cell lines. Here, using site directed mutagenesis, we investigate the role of the beta 2-adrenergic receptor phosphorylation by protein kinase C in this regulatory process. Mutation of the serine-261, -262, -344 a...

journal_title：FEBS letters

authors： Bouvier M,Guilbault N,Bonin H

更新日期：1991-02-25 00:00:00

abstract：:This study demonstrated that activation of tyrosine kinase of epidermal growth factor receptor (EGFR) induces its association with G protein-coupled receptor kinase 2 (GRK2). Immunoprecipitation experiments showed that EGF stimulation increased GRK2 binding to EGFR complex in HEK293 cells coexpressing EGFR and GRK2. T...

journal_title：FEBS letters

authors： Gao J,Li J,Chen Y,Ma L

更新日期：2005-01-03 00:00:00

abstract：:Complex I (NADH:ubiquinone oxidoreductase) is the first and largest protein complex of the oxidative phosphorylation. Crystal structures have elucidated the positions of most subunits of bacterial evolutionary origin in the complex, but the positions of the eukaryotic subunits are unknown. Based on the analysis of seq...

journal_title：FEBS letters

authors： Szklarczyk R,Wanschers BF,Nabuurs SB,Nouws J,Nijtmans LG,Huynen MA

更新日期：2011-03-09 00:00:00

abstract：:In human colon carcinoma HT-29 Glc(-/+) cells, L-arginine is the common precursor of polyamines which are absolutely necessary for cellular proliferation and nitric oxide (NO) with reported anti-proliferative activity. The aim of the present work was to test the effect of the NO donor sodium nitroprusside (SNP) on pol...

journal_title：FEBS letters

authors： Blachier F,Robert V,Selamnia M,Mayeur C,Duee PH

更新日期：1996-11-04 00:00:00

abstract：:Jeotgalicoccus sp. 8456 OleTJE (CYP152L1) is a fatty acid decarboxylase cytochrome P450 that uses hydrogen peroxide (H2 O2 ) to catalyse production of terminal alkenes, which are industrially important chemicals with biofuel applications. We report enzyme fusion systems in which Streptomyces coelicolor alditol oxidase...

journal_title：FEBS letters

authors： Matthews S,Tee KL,Rattray NJ,McLean KJ,Leys D,Parker DA,Blankley RT,Munro AW

更新日期：2017-03-01 00:00:00

abstract：:Dun1p and Rad53p of the budding yeast Saccharomyces cerevisiae are members of a conserved family of cell cycle checkpoint protein kinases that contain forkhead-associated (FHA) domains. Here, we demonstrate that these FHA domains contain 130-140 residues, and are thus considerably larger than previously predicted by s...

journal_title：FEBS letters

authors： Hammet A,Pike BL,Mitchelhill KI,Teh T,Kobe B,House CM,Kemp BE,Heierhorst J

更新日期：2000-04-14 00:00:00

abstract：:Contact inhibition of locomotion (CIL) occurs when a cell ceases moving in the same direction following contact with another cell. Homotypic and heterotypic CIL occur between cells of the same and different types, respectively. Using Abercrombie's confronted explants assay we studied the effect of changing Rac1 or Rho...

journal_title：FEBS letters

authors： Anear E,Parish RW

更新日期：2012-05-07 00:00:00

abstract：:Metabolic control theory is extended to include channelled metabolism in general. A simple relationship between the flux control by the enzymes and the degree of metabolite channelling is derived. This relationship suggests experiments in which modulation of gene expression allows one to quantify channelling. ...

journal_title：FEBS letters

authors： Kholodenko BN,Westerhoff HV

更新日期：1993-03-29 00:00:00

abstract：:The requirement of divalent cations for binding of the 46 kDa mannose 6-phosphate receptors to phosphomannan and pentamannose 6-phosphate-substituted bovine serum albumin was examined. Receptors from human liver and human brain bound to both affinity ligands in the absence or presence of divalent cations with similar ...

journal_title：FEBS letters

authors： Junghans U,Waheed A,von Figura K

更新日期：1988-09-12 00:00:00

abstract：:Fas ligand (FasL) binds Fas (CD95) to induce apoptosis or activate other signaling pathways. In addition, FasL transduces bidirectional or 'reverse signals'. The intracellular domain of FasL contains consensus sequences for phosphorylation and an extended proline rich region, which regulate its surface expression thro...

journal_title：FEBS letters

authors： Thornhill PB,Cohn JB,Drury G,Stanford WL,Bernstein A,Desbarats J

更新日期：2007-09-18 00:00:00

abstract：:Stomach infection with pathogenic strains of Helicobacter pylori causes in some patients severe gastroduodenal diseases. These bacteria produce various virulence factors and, here, we review the recent acquisition on the biochemical mode of action of three major factors. We discuss the role of urease both as buffer of...

journal_title：FEBS letters

authors： Montecucco C,Papini E,de Bernard M,Zoratti M

更新日期：1999-06-04 00:00:00

abstract：:In this study, we have identified 876 polymorphism sites in 145 complete or partial genomes of SARS-CoV available in the NCBI GenBank. One hundred and seventy-four of these sites existed in two or more SARS-CoV genome sequences. According to the sequence polymorphism, all SARS-CoVs can be divided into three groups: (I...

journal_title：FEBS letters

authors： Wang ZG,Zheng ZH,Shang L,Li LJ,Cong LM,Feng MG,Luo Y,Cheng SY,Zhang YJ,Ru MG,Wang ZX,Bao QY

更新日期：2005-09-12 00:00:00

abstract：:Mitochondria in primary living hepatocytes were visualized in cells transfected with a chimeric plasmid encoding for the green fluorescent protein (GFP) of Aequorea victoria engineered to be specifically targeted to mitochondria, as described recently (Rizutto et al. (1995) Curr. Biol. 5, 635-642). The identification ...

journal_title：FEBS letters

authors： Venerando R,Miotto G,Pizzo P,Rizzuto R,Siliprandi N

更新日期：1996-03-18 00:00:00

abstract：:When a cell divides prior to completion of DNA replication, serious DNA damage may occur. Thus, in addition to accuracy, the processivity of the replication forks is important. DNA synthesis at replication forks should be completed in time, and forks overcome aberrant structures on the template DNA, including damaged ...

journal_title：FEBS letters

authors： Hizume K,Araki H

更新日期：2019-07-01 00:00:00

abstract：:Gastric H+,K+-ATPase activity is not affected by Na+ at pH 7.0 but is significantly stimulated by Na+ at pH 8.5. For the stimulation at the latter pH, the presence of both Na+ and K+ were essential. Contrary the H+,K+-ATPase, the associated K+-pNPPase was inhibited by Na+ at both pH values. Sodium competes with K+ for...

journal_title：FEBS letters

authors： Ray TK,Nandi J

更新日期：1985-06-03 00:00:00

abstract：:Streptococcus pneumoniae (pneumococcus) has evolved sophisticated strategies to survive in several niches within the human body either as a harmless commensal or as a serious pathogen causing a variety of diseases. The dynamic interaction between pneumococci and resident host cells during colonization of the upper res...

journal_title：FEBS letters

authors： Kohler S,Voß F,Gómez Mejia A,Brown JS,Hammerschmidt S

更新日期：2016-11-01 00:00:00

abstract：:The proteolysis of beta-amyloid (Abeta) requires neprylisin, an enzyme that has been shown as reduced in Alzheimer's disease (AD). We investigated whether a decrease in neprilysin levels contributes to the accumulation of amyloid deposits not only in AD but also in the normal aging. We analyzed neprilysin mRNA and pro...

journal_title：FEBS letters

authors： Russo R,Borghi R,Markesbery W,Tabaton M,Piccini A

更新日期：2005-11-07 00:00:00

abstract：:By co-expression of heme oxygenase and various bilin reductase(s) in a single operon in conjunction with apophytochrome using two compatible plasmids, we developed a system to produce phytochromes with various chromophores in Escherichia coli. Through the selection of different bilin reductases, apophytochromes were a...

journal_title：FEBS letters

authors： Mukougawa K,Kanamoto H,Kobayashi T,Yokota A,Kohchi T

更新日期：2006-02-20 00:00:00

abstract：:The polyomavirus enhancer binding protein 2alpha (PEBP2alpha) is a DNA binding transcriptional regulatory protein that binds conserved sites in the polyomavirus enhancer, mammalian type C retroviral enhancers and T-cell receptor gene enhancers. Binding of PEBP2alpha and homologous proteins to the consensus DNA sequenc...

journal_title：FEBS letters

authors： Pérez-Alvarado GC,Munnerlyn A,Dyson HJ,Grosschedl R,Wright PE

更新日期：2000-03-24 00:00:00

abstract：:De novo synthesis of total and ribosomal ribonucleic acids has been studied during the early stages of Artemia sp. development. By in vivo incorporation studies of [14C]HCO3- an increase has been found in both total and ribosomal RNA synthesis post hatching, with a similar distribution of radioactivity and base compos...

journal_title：FEBS letters

authors： Liras A,Hernández Cruz MC,Rotllán P,Argomaniz L,Llorente P

更新日期：1989-09-25 00:00:00

abstract：:A one-electron reductive metabolism of 1,2-dibromoethane (DBE) is described that gives rise to a free radical intermediate, which can be stabilized by a spin trapping agent and detected by electron spin resonance spectroscopy. Using rat liver microsomes or isolated hepatocytes from phenobarbitone pretreated animals, u...

journal_title：FEBS letters

authors： Tomasi A,Albano E,Dianzani MU,Slater TF,Vannini V

更新日期：1983-08-22 00:00:00

abstract：:Evidence is presented here that cupric ions play a part in the natural spinning of Bombyx mori silk. Proton induced X-ray emission studies revealed that the copper content increased from the posterior part to the anterior part of silk gland, and then further increased in the silk fiber. Spectrophotometric analysis dem...

journal_title：FEBS letters

authors： Zhou L,Chen X,Shao Z,Zhou P,Knight DP,Vollrath F

更新日期：2003-11-20 00:00:00

abstract：:RNA-binding protein kinase from amphibian oocytes modifies serine and threonine residues in the molecules of substrates and utilizes both ATP and GTP. Low concentrations of heparin inhibit protein kinase. The foregoing suggests that this enzyme is casein kinase II. It is shown that RNA-binding proteins lack active for...

journal_title：FEBS letters

authors： Kandror KV,Stepanov AS

更新日期：1984-05-07 00:00:00

abstract：:Using reverse transcription followed by PCR amplification (RT-PCR), we have identified multiple messenger RNAs encoding for the neuronal pore-forming Ca(2+) channel subunits alpha(1A) (P/Q channel), alpha(1B) (N channel), alpha(1D) (neuronal/endocrine L channel), alpha(1E) (R channel), alpha(1G-H) (T channel) and alph...

journal_title：FEBS letters

authors： García-Palomero E,Cuchillo-Ibáñez I,García AG,Renart J,Albillos A,Montiel C

更新日期：2000-09-22 00:00:00

abstract：:The protein kinase D family of enzymes consists of three isoforms: PKD1/PKCmu PKD2 and PKD3/PKCnu. They all share a similar architecture with regulatory sub-domains that play specific roles in the activation, translocation and function of the enzymes. The PKD enzymes have recently been implicated in very diverse cellu...

journal_title：FEBS letters

authors： Rykx A,De Kimpe L,Mikhalap S,Vantus T,Seufferlein T,Vandenheede JR,Van Lint J

更新日期：2003-07-03 00:00:00

abstract：:Fatty acid (FA) uniport via mitochondrial uncoupling protein (UcP) was detected fluorometrically with PBFI, potassium-binding benzofuran phthalate and SPQ, 6-methoxy-N-(3-sulfopropyl)-quinolinium, indicating K+ and H+, respectively. The FA structural patterns required for FA flip-flop, UcP-mediated FA uniport, activat...

journal_title：FEBS letters

authors： Jezek P,Modrianský M,Garlid KD

更新日期：1997-05-19 00:00:00

abstract：:We report the identification, gene expression and biological activity of two parathyroid hormones (PTH; PTHA and PTHB), two PTH-related peptides (PTHrP; PTHrPA and PTHrPB) and a PTH-like ligand (PTH-L) with hybrid characteristics in puffer fishes (Takifugu rubripes and Tetraodon fluviatilis). Experimental data are con...

journal_title：FEBS letters

authors： Canario AV,Rotllant J,Fuentes J,Guerreiro PM,Rita Teodósio H,Power DM,Clark MS

更新日期：2006-01-09 00:00:00

abstract：:The retromer complex is a key element of the endosomal protein sorting machinery that is conserved through evolution and has been shown to play a role in diseases such as Alzheimer's disease and Parkinson's disease. Through sorting various membrane proteins (cargo), the function of retromer complex has been linked to ...

journal_title：FEBS letters

authors： Mukadam AS,Seaman MN

更新日期：2015-09-14 00:00:00

abstract：:This study was designed to investigate changes in gene expression associated with stage-specific programmed cell death (PCD) in intersegmental muscles (ISMs) of the moth, Manduca sexta. The technique of differential display reverse transcription PCR was applied to compare mRNA levels before and after the onset of PCD ...

journal_title：FEBS letters

authors： Löw P,Talián GC,Sass M

更新日期：2005-09-12 00:00:00