Abstract:
:Synthetic oligonucleotides have been used to isolate a 1.85 kb clone containing the full length coding sequence for the catalytic subunit of rabbit skeletal muscle phosphorylase kinase from a cDNA library constructed in lambda gt10. Sequence analysis of the clone predicted an amino acid sequence in agreement with a published primary structure. Inspection of the codon usage revealed a strong preference for G or C nucleotides at the third codon position as found for several other skeletal muscle proteins. This cDNA clone should facilitate identification of functional domains, including the calmodulin-binding site, and investigation of the molecular basis of X-linked phosphorylase kinase deficiencies.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
da Cruz e Silva EF,Cohen PTdoi
10.1016/0014-5793(87)80871-2subject
Has Abstractpub_date
1987-08-10 00:00:00pages
36-42issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(87)80871-2journal_volume
220pub_type
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