Abstract:
:Ribosomes are ribozymes exerting substrate positioning and promoting substrate-mediated catalysis. Peptide-bonds are formed within a symmetrical region, thus suggesting that ribosomes evolved by gene-fusion. Remote interactions dominate substrate positioning at stereochemistry suitable for peptide-bond formation and elaborate architectural-design guides the processivity of the reaction by rotatory motion. Nascent proteins are directed into the exit tunnel at extended conformation, complying with the tunnel's narrow entrance. Tunnel dynamics facilitate its interactive participation in elongation, discrimination, cellular signaling and nascent-protein trafficking into the chaperon-aided folding site. Conformational alterations, induced by ribosomal-recycling factor, facilitate subunit dissociation. Remarkably, although antibiotics discrimination is determined by the identity of a single nucleotide, involved also in resistance, additional nucleotides dictate antibiotics effectiveness.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Baram D,Yonath Adoi
10.1016/j.febslet.2004.11.063keywords:
subject
Has Abstractpub_date
2005-02-07 00:00:00pages
948-54issue
4eissn
0014-5793issn
1873-3468pii
S0014-5793(04)01457-7journal_volume
579pub_type
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