Cloning and high-level expression of a chloroperoxidase gene from Pseudomonas pyrrocinia in Escherichia coli.

abstract：:It was of interest to examine whether yeast Cu-thionein could be used to transfer the thiolate bound copper directly into the copper binding site of 'blue' apoproteins which contain free thiol groups. In particular apo-stellacyanin was used in the present study and it was found to be able to accept Cu(I) from yeast Cu...

journal_title：FEBS letters

authors： Hartmann HJ,Morpurgo L,Desideri A,Rotilio G,Weser U

更新日期：1983-02-07 00:00:00

abstract：:A structure of residues 1-177 of the cyclophilin domain of a large divergent cyclophilin from the filarial nematode parasite Brugia malayi has been crystallised and solved in two different crystal forms. The active site has a similar structure to that of human cyclophilin A. Two of the 13 residues important in forming...

journal_title：FEBS letters

authors： Taylor P,Page AP,Kontopidis G,Husi H,Walkinshaw MD

更新日期：1998-03-27 00:00:00

abstract：:L- and D-specific nicotinamide adenine dinucleotide (NAD)-dependent dehydrogenases map to the same structural protein superfamily as defined by the Structural Classification of Proteins (SCOP) and are based on the Rossmann fold type domains. A detailed classification of these domains is proposed using a novel diagnost...

journal_title：FEBS letters

authors： Kutzenko AS,Lamzin VS,Popov VO

更新日期：1998-02-13 00:00:00

abstract：:Fibromodulin and lumican are closely related members of the extracellular matrix leucine-rich repeat glycoprotein/proteoglycan family. Similar to decorin, another member of this protein family, they bind to fibrillar collagens and function in the assembly of the collagen network in connective tissues. We have studied ...

journal_title：FEBS letters

authors： Svensson L,Närlid I,Oldberg A

更新日期：2000-03-24 00:00:00

abstract：:Recent advances in our understanding of the structure of highly amplified DNA sequences in Streptomyces fradiae and lividans have enabled us to formulate a possible mechanism by which amplification may occur. An essential feature of the model is the generation of an amplification precursor, which comprises a circulari...

journal_title：FEBS letters

authors： Young M,Cullum J

更新日期：1987-02-09 00:00:00

abstract：:The amyloid precursor protein (APP) is proteolytically processed by β- and γ-secretases to release amyloid-β peptide (Aβ), the main component found in senile plaques of Alzheimer's disease (AD) patient brains. Alternatively, APP can be cleaved within the Aβ sequence by α-secretase, thus precluding the generation of Aβ...

journal_title：FEBS letters

authors： León-Otegui M,Gómez-Villafuertes R,Díaz-Hernández JI,Díaz-Hernández M,Miras-Portugal MT,Gualix J

更新日期：2011-07-21 00:00:00

abstract：:Human cystatin C (HCC) is a low molecular weight member of the cystatin family (type2). HCC consists of 120 amino acids. Normally it is an inhibitor of cysteine proteases, but in pathological conditions it forms amyloid fibrils in brain arteries of young adults. An 'aggregation-prone' pentapeptide ((47)LQVVR(51)) was ...

journal_title：FEBS letters

authors： Tsiolaki PL,Hamodrakas SJ,Iconomidou VA

更新日期：2015-01-02 00:00:00

abstract：:A cytosolic calmodulin wave has been described in the pre-replicative phase of rat liver regeneration. Here we demonstrate that this calmodulin increase is reduced by injection of cycloheximide (translation inhibitor) and actinomycin D (transcription inhibitor). In addition, we found that calmodulin mRNA levels are in...

journal_title：FEBS letters

authors： Piõl MR,Berchtold MW,Bachs O,Heizmann CW

更新日期：1988-04-25 00:00:00

abstract：:Specific binding sites for 3,3'-T2 can be detected in swollen and osmotically treated mitochondria (OTM) from normal and hypothyroid rat liver. In hypothyroid animals, maximal values of binding were obtained at 0 degrees C while values were lower at 37 degrees C and no specific binding could be observed at 60 degrees ...

journal_title：FEBS letters

authors： Lanni A,Moreno M,Horst C,Lombardi A,Goglia F

更新日期：1994-09-05 00:00:00

abstract：:In human platelet membranes enhanced tyrosine phosphorylation of certain proteins was observed when Zn2+ instead of Mg2+ or Mn2+ was used as a divalent cation for the kinase reaction. An enhanced level of phosphate incorporation into tyrosine residues occurred into a 68 kDa polypeptide besides the 45 kDa and 105 kDa p...

journal_title：FEBS letters

authors： Findik D,Presek P

更新日期：1988-08-01 00:00:00

abstract：:Large amounts of refined sequence material in the form of predicted, curated and annotated genes and expressed sequences tags (ESTs) have recently been added to the NCBI databases. We matched the transcript-sequences of RefSeq, Ensembl and dbEST in an attempt to provide an updated overview of how many unique human gen...

journal_title：FEBS letters

authors： Larsson TP,Murray CG,Hill T,Fredriksson R,Schiöth HB

更新日期：2005-01-31 00:00:00

abstract：:The class III phosphatidylinositol 3-kinase (PI3K-III) complex and its phosphorylated lipid product phosphatidylinositol 3-phosphate (PtdIns3P) control the three topologically related membrane-involution processes autophagy, endocytosis, and cytokinesis. The activity of the catalytic unit of PI3K-III complex, the Vacu...

journal_title：FEBS letters

authors： Abrahamsen H,Stenmark H,Platta HW

更新日期：2012-06-04 00:00:00

abstract：:Sterically hindered cyclic amines react with hydroxyl radicals in the presence of oxygen to yield stable nitroxide radicals which can be detected by EPR. This reaction provides a nonconventional spin-trapping tool for detection of hydroxyl radicals. ...

journal_title：FEBS letters

authors： Rosenthal I,Krishna CM,Yang GC,Kondo T,Riesz P

更新日期：1987-09-28 00:00:00

abstract：:Jeotgalicoccus sp. 8456 OleTJE (CYP152L1) is a fatty acid decarboxylase cytochrome P450 that uses hydrogen peroxide (H2 O2 ) to catalyse production of terminal alkenes, which are industrially important chemicals with biofuel applications. We report enzyme fusion systems in which Streptomyces coelicolor alditol oxidase...

journal_title：FEBS letters

authors： Matthews S,Tee KL,Rattray NJ,McLean KJ,Leys D,Parker DA,Blankley RT,Munro AW

更新日期：2017-03-01 00:00:00

abstract：:The maintenance of membrane lipid composition within strict limits is critical to maintain optimum cellular function. The biophysical properties of the membrane can be influenced among other factors by the saturation/unsaturation of the phospholipid fatty acyl chain. The rate-limiting enzyme in unsaturated fatty acid ...

journal_title：FEBS letters

authors： Hagen RM,Rodriguez-Cuenca S,Vidal-Puig A

更新日期：2010-06-18 00:00:00

abstract：:The alpha-subunit of the TRAP complex (TRAP alpha) is a single-spanning membrane protein of the endoplasmic reticulum (ER) which is found in proximity of nascent polypeptide chains translocating across the membrane. Here, we demonstrate the widespread occurrence of TRAP alpha in eukaryotes as indicated by its existenc...

journal_title：FEBS letters

authors： Hartmann E,Prehn S

更新日期：1994-08-08 00:00:00

abstract：:Saccharomyces cerevisiae Vig4/Vrg4 protein is a Golgi membrane protein which has multiple transmembrane domains and is essential for transport of GDP-mannose across the Golgi membrane. By immunoprecipitation of detergent-solubilized tagged protein, we found that this protein exists as oligomer. Two mutants vig4-1 and ...

journal_title：FEBS letters

authors： Abe M,Hashimoto H,Yoda K

更新日期：1999-09-24 00:00:00

abstract：:Heat shock proteins (Hsps) are induced in response to various kinds of environmental and physiological stresses. However, it is unclear whether Hsps play roles in protecting cells in the digestive organs against xenobiotic chemicals. Here, we found that feeding induces expression of a set of Hsps specifically in the m...

journal_title：FEBS letters

authors： Katsuki K,Fujimoto M,Zhang XY,Izu H,Takaki E,Tanizawa Y,Inouye S,Nakai A

更新日期：2004-07-30 00:00:00

abstract：:The interferon inducible transmembrane (IFITM) proteins mediate several cellular processes such as homotypic cell adhesion functions of interferons (IFNs) and cellular anti-proliferative activities. We show that the BAF complex-mediated induction of IFITM3 is dependent on binding of the transcriptional enhancer factor...

journal_title：FEBS letters

authors： Cuddapah S,Cui K,Zhao K

更新日期：2008-01-23 00:00:00

abstract：:Chicken muscular dystrophy with abnormal muscle (AM) has been studied for more than 50 years, but the gene responsible for it remains unclear. Our previous studies narrowed down the AM candidate region to approximately 1Mbp of chicken chromosome 2q containing seven genes. In this study, we performed sequence compariso...

journal_title：FEBS letters

authors： Matsumoto H,Maruse H,Inaba Y,Yoshizawa K,Sasazaki S,Fujiwara A,Nishibori M,Nakamura A,Takeda S,Ichihara N,Kikuchi T,Mukai F,Mannen H

更新日期：2008-06-25 00:00:00

abstract：:The relationship between different amino acids at the beta 6 position of hemoglobin and tetramer stability was addressed by a site-directed mutagenesis approach. Precipitation rates during mechanical agitation of oxyhemoglobins with Gln, Ala, Val, Leu and Trp at the beta 6 position increased 2, 5, 13, 21 and 53 times,...

journal_title：FEBS letters

authors： Adachi K,Kim JY,Konitzer P,Asakura T,Saviola B,Surrey S

更新日期：1993-01-02 00:00:00

abstract：:Stimuli which enhance secretion from parathyroid cells such as low extracellular Ca2+ or Mg2+ are associated with a decrease in the cytosolic Ca2+ concentration as measured by quin2. Current evidence suggests that increased production of inositol 1,4,5-triphosphate (IP3) releases Ca2+ from cellular stores thus increas...

journal_title：FEBS letters

authors： Epstein PA,Prentki M,Attie MF

更新日期：1985-08-19 00:00:00

abstract：:A rat urokinase receptor (uPAR) cDNA fragment was amplified by RT-PCR from RNA of the rat osteoblastic cell line CFK-1. Using this DNA species as a hybridization probe two rat uPAR cDNAs were isolated from a CFK-1 cDNA library. These two cDNAs encode an identical uPAR protein except for a single base mutation which re...

journal_title：FEBS letters

authors： Rabbani SA,Rajwans N,Achbarou A,Murthy KK,Goltzman D

更新日期：1994-01-24 00:00:00

abstract：:Different bioinformatics methods illuminate different aspects of protein function, from specific catalytic activities to broad functional categories. Here, a triple-pronged approach to predict function for a domain of unknown function, DUF2086, is applied. Distant homology to characterised enzymes and conservation of ...

journal_title：FEBS letters

authors： Mello LV,Rigden DJ

更新日期：2012-11-02 00:00:00

abstract：:The selectively red excited emission spectrum, at room temperature, of the in vitro reconstituted Lhca4, has a pronounced non-equilibrium distribution, leading to enhanced emission from the directly excited low-energy pigments. Two different emitting forms (or states), with maximal emission at 713 and 735nm (F713 and ...

journal_title：FEBS letters

authors： Zucchelli G,Morosinotto T,Garlaschi FM,Bassi R,Jennings RC

更新日期：2005-04-11 00:00:00

abstract：:DNA polymerase alpha-primase is a heterotetrameric complex essential for simian vacuolating virus 40 (SV40) DNA replication. We show that the C-terminal 67 amino acid residues of the human p180 subunit are essential for SV40 DNA replication as they are required for binding of the p68 subunit and play a role in the int...

journal_title：FEBS letters

authors： Smith RW,Nasheuer HP

更新日期：2002-09-11 00:00:00

abstract：:The Fas/Fas ligand interaction plays a crucial role in various liver diseases, and administration of agonistic anti-Fas antibody to mice causes massive hepatic apoptosis and fulminant hepatic failure. Several growth factors have recently been found to function in preventing apoptosis. In this study, we demonstrated th...

journal_title：FEBS letters

authors： Kanda D,Takagi H,Toyoda M,Horiguchi N,Nakajima H,Otsuka T,Mori M

更新日期：2002-05-22 00:00:00

abstract：:A gene encoding a single-chain antibody fragment directed against digoxin (named 1C10 scFv) was cloned in two expression systems. For this purpose, a new baculovirus transfer cassette fully compatible with the procaryotic pHEN vector was constructed. Baculovirus production led to higher yield than did Escherichia coli...

journal_title：FEBS letters

authors： Lemeulle C,Chardès T,Montavon C,Chaabihi H,Mani JC,Pugnière M,Cerutti M,Devauchelle G,Pau B,Biard-Piechaczyk M

更新日期：1998-02-20 00:00:00

abstract：:This article presents an overview of the simulation studies of the behaviour of multihaem cytochromes using theoretical/computational methodologies, with an emphasis on cytochrome c(3). It starts with the first studies using rigid molecules and continuum electrostatic models, where protonation and redox events were tr...

journal_title：FEBS letters

authors： Soares CM,Baptista AM

更新日期：2012-03-09 00:00:00

abstract：:A UV irradiation-induced mutant of Bacillus sphaericus 2362 whose sporulation was inhibited neither by natural amino acids nor by L-ethionine was selected. The mutant (A61) grew slowly in rich amino acid medium and contained increased concentrations of heat-resistant spores throughout the growth. Slow growth of A61 wa...

journal_title：FEBS letters

authors： Andreev J,Dibrov PA,Braun S

更新日期：1994-06-27 00:00:00