Abstract:
:Hordeum vulgare L. yellow stripe 1 (HvYS1) is a selective transporter for Fe(III)-phytosiderophores, involved in primary iron acquisition from soils in barley roots. In contrast, Zea mays yellow stripe 1 (ZmYS1) in maize possesses broad substrate specificity, despite a high homology with HvYS1. Here we revealed, by assessing the transport activity of a series of HvYS1-ZmYS1 chimeras, that the outer membrane loop between the sixth and seventh transmembrane regions is essential for substrate specificity. Circular dichroism spectra indicated that a synthetic peptide corresponding to the loop of HvYS1 forms an alpha-helix in solution, whereas that of ZmYS1 is flexible. We propose that the structural difference at this particular loop determines the substrate specificity of the HvYS1 transporter.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Harada E,Sugase K,Namba K,Iwashita T,Murata Ydoi
10.1016/j.febslet.2007.08.011subject
Has Abstractpub_date
2007-09-04 00:00:00pages
4298-302issue
22eissn
0014-5793issn
1873-3468pii
S0014-5793(07)00881-2journal_volume
581pub_type
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