Single molecule approaches to transcription factor kinetics in living cells.

abstract：:Arf proteins are guanine nucleotide binding proteins that are implicated in endocytotic pathways and vesicle trafficking. The two widely studied isoforms of Arf proteins (Arf1 and Arf6) have different cellular functions and localizations but similar structures. Arf proteins have an N-terminal helix with a covalently b...

journal_title：FEBS letters

authors： Gizachew D,Oswald R

更新日期：2006-07-24 00:00:00

abstract：:It is shown that the proteins encoded by the tumor suppressor fat gene, the neurogenic slit gene and crumbs gene of Drosophila contain domains homologous with modules identified previously in laminin A. These proteins of Drosophila have a number of features in common: they have large extracellular regions containing l...

journal_title：FEBS letters

authors： Patthy L

更新日期：1992-02-24 00:00:00

abstract：:Bispecific miniantibodies were constructed by genetically fusing the C(H)1 domain of an IgG1 to the C-terminus of a single-chain Fv fragment (scFv-425), specific for the EGF receptor, and fusing the C(L) domain of a kappa light chain to the C-terminus of a scFv specific for CD2 (scFv-M1). An efficient dicistronic gene...

journal_title：FEBS letters

authors： Müller KM,Arndt KM,Strittmatter W,Plückthun A

更新日期：1998-01-30 00:00:00

abstract：:Recently the identity of the peptidyl-prolyl cis-trans isomerase (PPIase), which accelerates the cis/trans isomerization of prolyl peptide bonds and cyclophilin, the binding protein for the immunosuppressive drug Cyclosporin A (CsA), was discovered. The PPIase catalysis toward the substrate Suc-Ala-Phe-Pro-Phe-pNA has...

journal_title：FEBS letters

authors： Hübner D,Drakenberg T,Forsén S,Fischer G

更新日期：1991-06-17 00:00:00

abstract：:The intermediate in the reaction catalyzed by 4-chlorobenzoate dehalogenase from Pseudomonas sp. CBS3 was identified as 4-chlorobenzoyl-CoA. One component of 4-chlorobenzoate dehalogenase worked as a a 4-chlorobenzoyl-CoA ligase catalyzing the formation of 4-chlorobenzoyl-CoA from 4-chlorobenzoate, coenzyme A and ATP....

journal_title：FEBS letters

authors： Löffler F,Müller R

更新日期：1991-09-23 00:00:00

abstract：:We found that, in the presence of chimeric oligonucleotides containing complementary deoxyribo- and 2'-O-methylnucleosides, a nonaribonucleotide, [5'-32P]pACUUACCUG, was cleaved specifically upon treatment with RNase H. When 3'm(UG)d(AATG)m(GAC)5' was used as a hybridization strand, pACUUACCUG was cleaved between C6 a...

journal_title：FEBS letters

authors： Inoue H,Hayase Y,Iwai S,Ohtsuka E

更新日期：1987-05-11 00:00:00

abstract：:In presynaptic nerve terminals, synaptic vesicles are recycled locally via an evolutionarily conserved process that ensures maintenance of neurotransmission as well as structural integrity of synapses. Temperature is a key environmental factor that impacts critical steps involved in fusion, endocytosis and transport i...

journal_title：FEBS letters

authors： Chanaday NL,Kavalali ET

更新日期：2018-11-01 00:00:00

abstract：:Familial porphyria cutanea tarda (PCT) is a photocutaneous disease in which subnormal activity of uroporphyrinogen decarboxylase is observed both in the liver and red cells. Hepatic iron plays a key role in the genesis of overt biochemical and clinical PCT. In this report, we have studied the properties of 10 000-fold...

journal_title：FEBS letters

authors： Mukerji SK,Pimstone NR,Tan KT

更新日期：1985-09-23 00:00:00

abstract：:This study was performed to prove our hypothesis that the metabolite(s) of polycyclic aromatic hydrocarbons (PAHs) caused the activation or phosphorylation of p53 via DNA damage to suppress the liver X receptor (LXR)-mediated signal transductions as a probably more direct mechanism. We found that LXR-mediated trans-ac...

journal_title：FEBS letters

authors： Iwano S,Shibahara N,Saito T,Kamataki T

更新日期：2006-02-06 00:00:00

abstract：:A key step in sulfate assimilation into cysteine is the reduction of sulfite to sulfide by sulfite reductase (SiR). This enzyme is encoded by three genes in the moss Physcomitrella patens. To obtain a first insight into the roles of the individual isoforms, we deleted the gene encoding the SiR1 isoform in P. patens by...

journal_title：FEBS letters

authors： Wiedemann G,Hermsen C,Melzer M,Büttner-Mainik A,Rennenberg H,Reski R,Kopriva S

更新日期：2010-06-03 00:00:00

abstract：:A developmentally regulated exon has been identified in the 5'-alternatively spliced region of the human fast Troponin T (TnT) gene. Expressed in fetal (but not adult) muscle, this exon is homologous with the fetal exons recently described in the rabbit and rat fast TnT genes. They all exhibit a split codon organizati...

journal_title：FEBS letters

authors： Briggs MM,Maready M,Schmidt JM,Schachat F

更新日期：1994-08-15 00:00:00

abstract：:Stimulation of cultured pituitary cells from a gonadotrope lineage (alpha T3-1) by the gonadotropin-releasing hormone agonist analog [D-Trp6]GnRH (GnRH-A) resulted in a manifold increase in accumulation of phosphatidylethanol, a specific product of phospholipase D phosphatidyl transferase activity when ethanol is the ...

journal_title：FEBS letters

authors： Netiv E,Liscovitch M,Naor Z

更新日期：1991-12-16 00:00:00

abstract：:Photon correlation spectroscopy demonstrated for the first time that co-purified meningococcal TbpA+B form a complex in solution. This structure bound hTf and the resultant species underwent partial dissociation after exposure to additional hTf or following prolonged incubation. Purified TbpA and TbpB had similar appa...

journal_title：FEBS letters

authors： Boulton IC,Gorringe AR,Carr RJ,Gorinsky B,Joannou CL,Evans RW

更新日期：1997-09-08 00:00:00

abstract：:The aim of this work was to provide an overview of the genetics of human ageing to gain novel insights about the mechanisms involved. By incorporating findings from model organisms to humans, such as mutations that either delay or accelerate ageing in mice, we constructed the gene networks previously related to ageing...

journal_title：FEBS letters

authors： de Magalhães JP,Toussaint O

更新日期：2004-07-30 00:00:00

abstract：:Phlorizin, a potent inhibitor of the Na+/D-glucose cotransporter, was derivatised to 3-aminophlorizin and subsequently coupled to Affi-Gel 15. Affinity chromatography of pig kidney brush border membranes solubilised in Triton X-100 allowed the purification of a 60 kDa protein on this resin. We consider this protein to...

journal_title：FEBS letters

authors： Kitlar T,Morrison AI,Kinne R,Deutscher J

更新日期：1988-07-04 00:00:00

abstract：:The interaction of Bordetella pertussis adenylate cyclase toxin (CyaA) with complement receptor 3 (CR3, CD11b/CD18) involves N-linked oligosaccharide chains. To investigate the relative importance of the individual N-glycans of CR3 for toxin activity, the asparagine residues of the consensus N-glycosylation sites of C...

journal_title：FEBS letters

authors： Hasan S,Osickova A,Bumba L,Novák P,Sebo P,Osicka R

更新日期：2015-01-30 00:00:00

abstract：:Cytochrome c oxidase is a membrane-bound redox-driven proton pump that harbors two proton-transfer pathways, D and K, which are used at different stages of the reaction cycle. Here, we address the question if a D pathway with a modified energy landscape for proton transfer could take over the role of the K pathway whe...

journal_title：FEBS letters

authors： Vilhjálmsdóttir J,Albertsson I,Blomberg MRA,Ädelroth P,Brzezinski P

更新日期：2020-03-01 00:00:00

abstract：:Using ultrastructural analysis and labeling with polyclonal antibodies that recognize peptide sequences specific for phospholipid binding, we mapped the functional domain structure of intact platelet talin and its proteolytic fragments. The talin dimer, which is crucial for actin and lipid binding, is built of a backb...

journal_title：FEBS letters

authors： Isenberg G,Goldmann WH

更新日期：1998-04-17 00:00:00

abstract：:Proline-rich tyrosine kinase2 (Pyk2) is a cytoplasmic tyrosine kinase related to focal adhesion kinase. Pyk2 expression has been known to be restricted to neuronal and hematopoietic cells and Pyk2 tyrosine phosphorylation and its kinase activity is important for the function of monocytes/macrophages. In NB4 acute prom...

journal_title：FEBS letters

authors： Park MH,Park SY,Kim Y

更新日期：2008-02-06 00:00:00

abstract：:Calpain, a Ca2+-dependent biomodulator, alters the properties of substrate proteins by cleaving them at a limited number of specific sites. Recent studies of the structure-function relationship of calpain and X-ray analysis of its Ca2+-binding domain have revealed hitherto unknown features of the regulation of calpain...

journal_title：FEBS letters

authors： Suzuki K,Sorimachi H

更新日期：1998-08-14 00:00:00

abstract：:The immunosuppressive agent FK-506 has received much attention due to its efficacy and potency in the areas of transplant rejection and autoimmune disease. Calcineurin, a Ca(2+)-calmodulin activated phosphatase, was recently implicated in the immunosuppressive mechanism of FK-506. In our ongoing search for superior im...

journal_title：FEBS letters

authors： Kawai M,Lane BC,Hsieh GC,Mollison KW,Carter GW,Luly JR

更新日期：1993-01-25 00:00:00

abstract：:Tyrosine phosphorylation of Cbl and its association with signal-transducing molecules in response to macrophage colony-stimulating factor (M-CSF) were analyzed by using cell lines which express the wild-type and a mutant M-CSF receptor, Fms. We found that in a clone, F723 TF-1 cells expressing mutant Fms in which tyro...

journal_title：FEBS letters

authors： Ota J,Sato K,Kimura F,Wakimoto N,Nakamura Y,Nagata N,Suzu S,Yamada M,Shimamura S,Motoyoshi K

更新日期：2000-01-21 00:00:00

abstract：:We examined the role of p38 mitogen-activated protein (MAP) kinase in the tumor necrosis factor alpha (TNF-alpha)- or interleukin-1beta (IL-1beta)-induced production of interleukin-6 (IL-6) and interleukin-8 (IL-8) in fresh rheumatoid synovial fibroblast (RSF) cultures concomitantly with the induction of p38 MAP kinas...

journal_title：FEBS letters

authors： Suzuki M,Tetsuka T,Yoshida S,Watanabe N,Kobayashi M,Matsui N,Okamoto T

更新日期：2000-01-07 00:00:00

abstract：:To search for negative regulatory components of the NF-kappaB activation pathways, we mutagenized Rat-1 fibroblasts and established a stable mutant cell line with a constitutive NF-kappaB activity. This mutant cell line, designated as TK26, showed permanently elevated I kappa B kinase (IKK) activity and a genetically ...

journal_title：FEBS letters

authors： Chinanonwait N,Miura H,Yamamoto N,Yamaoka S

更新日期：2002-11-20 00:00:00

abstract：:The amino acid residue Asn141 of the restriction endonuclease EcoRI was proposed to make three hydrogen bonds to both adenine residues within the recognition sequence -GAATTC-. We have mutated Asn141 to alanine, aspartate, serine, and tyrosine. Only the serine mutant is active under normal buffer conditions although 1...

journal_title：FEBS letters

authors： Fritz A,Küster W,Alves J

更新日期：1998-10-30 00:00:00

abstract：:The N-terminus of MDM2 proto-oncoprotein interacts with p53 and down modulates p53 activity by inhibiting transcriptional activity and promoting p53 degradation. MDMX is structurally related to MDM2 and also binds to p53. However, the function of MDMX has not been clarified yet. We found that MDM2 hetero-oligomerized ...

journal_title：FEBS letters

authors： Tanimura S,Ohtsuka S,Mitsui K,Shirouzu K,Yoshimura A,Ohtsubo M

更新日期：1999-03-19 00:00:00

abstract：:Lymphotoxin-beta receptor (LTbetaR) is a member of the tumor necrosis factor receptor (TNFR) superfamily that activates nuclear factor-kappaB (NF-kappaB) through the IkappaB kinase (IKK) complex, the core of which is comprised of IKK1, IKK2 and NF-kappaB essential modulator (NEMO). We demonstrate here that the LTbetaR...

journal_title：FEBS letters

authors： Saitoh T,Nakano H,Yamamoto N,Yamaoka S

更新日期：2002-12-04 00:00:00

abstract：:Single channel recording techniques have been applied to neurons cultured from the hippocampus and the respiratory area of the brain stem of fetal rats in order to search for nicotinic acetylcholine receptors (nAChR) in the central nervous system. In addition to acetylcholine (ACh), the potent and specific agonist (+)...

journal_title：FEBS letters

authors： Aracava Y,Deshpande SS,Swanson KL,Rapoport H,Wonnacott S,Lunt G,Albuquerque EX

更新日期：1987-09-28 00:00:00

abstract：:Signaling via G-protein coupled receptors is initiated by receptor-catalyzed nucleotide exchange on Galpha subunits normally bound to GDP and Gbetagamma. Activated Galpha . GTP then regulates effectors such as adenylyl cyclase. Except for Gbetagamma, no known regulators bind the adenylyl cyclase-stimulatory subunit Ga...

journal_title：FEBS letters

authors： Johnston CA,Ramer JK,Blaesius R,Fredericks Z,Watts VJ,Siderovski DP

更新日期：2005-10-24 00:00:00

abstract：:Treatment of yeast phosphoglycerate kinase (PGK) with trypsin results in a fourfold increase in the Vmax of this enzyme, without affecting the Km. This activation is shown to be due to the removal of the C-terminal lysine residue. The C-terminal sequence folds back over the N-terminal domain and contacts the extreme N...

journal_title：FEBS letters

authors： Adams B,Fowler R,Hudson M,Pain RH

更新日期：1996-04-29 00:00:00