Identification of 4-chlorobenzoyl-coenzyme A as intermediate in the dehalogenation catalyzed by 4-chlorobenzoate dehalogenase from Pseudomonas sp. CBS3.

abstract：:Coronin proteins are widely expressed among eukaryotic organisms. Most coronins consist of a WD-repeat domain followed by a C-terminal coiled coil. Dictyostelium discoideum expresses a single short coronin coronin A, which has been implicated in both actin modulation and multicellular differentiation. Whether coronin ...

journal_title：FEBS letters

authors： Fiedler T,Fabrice TN,Studer V,Vinet A,Faltova L,Kammerer RA,Steinmetz MO,Sharpe T,Pieters J

更新日期：2020-04-16 00:00:00

abstract：:The orientation of the transition dipole moments in the ground state and the M-intermediate of bacteriorhodopsin were determined by time-resolved and steady-state polarized absorption spectroscopy on samples of oriented immobilized purple membranes. The angle between the transition dipole moment and the membrane norma...

journal_title：FEBS letters

authors： Otto H,Heyn MP

更新日期：1991-11-18 00:00:00

abstract：:Benzimidazoles are widely used as anthelmintic agents and systemic fungicides. In susceptible organisms, benzimidazoles bind to beta-tubulin and block microtubule polymerization. To further characterize this interaction, site-directed mutagenesis followed by gene replacement was used to change Saccharomyces cerevisiae...

journal_title：FEBS letters

authors： Li J,Katiyar SK,Edlind TD

更新日期：1996-04-29 00:00:00

abstract：:Protein kinases play a crucial role in the regulation of cellular processes. Most eukaryotes reserve about 2.5% of their genes for protein kinases. We analysed the genome of the single-celled ciliate Paramecium tetraurelia and identified 2606 kinases, about 6.6% of its genes, representing the largest kinome to date. A...

journal_title：FEBS letters

authors： Bemm F,Schwarz R,Förster F,Schultz J

更新日期：2009-11-19 00:00:00

abstract：:Absence through mutation of two proteins involved in periplasmic disulphide bond formation, DsbA and DsbB, results in failure of anaerobically grown Escherichia coli to synthesise the holo forms of either its endogenous c-type cytochrome nitrite reductase or exogenous cytochrome c550 from Paracoccus denitrificans. The...

journal_title：FEBS letters

authors： Sambongi Y,Ferguson SJ

更新日期：1996-12-02 00:00:00

abstract：:The known members of inhibitor of growth (ING) gene family are considered as candidate tumor suppressor genes. ING4, a novel member of ING family, is recently reported to interact with tumor suppressor p53, p300 (a major component of histone acetyl transferase complexes), and p65(RelA) subunit of NF-kappaB. In this st...

journal_title：FEBS letters

authors： Zhang X,Xu LS,Wang ZQ,Wang KS,Li N,Cheng ZH,Huang SZ,Wei DZ,Han ZG

更新日期：2004-07-16 00:00:00

abstract：:The dynamics of changes in membrane potential, cytosolic free Ca2+, [Ca2+]i and immunoreactive insulin release were assessed in RINm5F cells. Membrane depolarization and a rise in [Ca2+]i preceded the stimulation of insulin release by D-glyceraldehyde. Forskolin, which raised the cellular cyclic AMP levels, stimulated...

journal_title：FEBS letters

authors： Wollheim CB,Ullrich S,Pozzan T

更新日期：1984-11-05 00:00:00

abstract：:An essential component of the chromatin remodeling machinery is NURF (Nucleosome Remodeling Factor), the founding member of the ISWI family of chromatin remodeling complexes. In vertebrates and invertebrates alike, NURF has many important functions in chromatin biology including regulating transcription, establishing ...

journal_title：FEBS letters

authors： Alkhatib SG,Landry JW

更新日期：2011-10-20 00:00:00

abstract：:On April 1898 Camillo Golgi communicated to the Medical-Surgical Society of Pavia, the discovery of the "internal reticular apparatus", a novel intracellular organelle which he observed in nerve cells with the silver impregnation he had introduced for the staining of the nervous system. Soon after the discovery it bec...

journal_title：FEBS letters

authors： Mazzarello P,Garbarino C,Calligaro A

更新日期：2009-12-03 00:00:00

abstract：:The transdifferentiation of hepatic stellate cells into myofibroblast-like cells and the proliferation of the transdifferentiated cells are controlled by TGF-beta1. Little is known about the intracellular signal transducers of TGF-beta1. In this paper we show that in cultured hepatic stellate cells TGF-beta1 induces a...

journal_title：FEBS letters

authors： Reimann T,Hempel U,Krautwald S,Axmann A,Scheibe R,Seidel D,Wenzel KW

更新日期：1997-02-10 00:00:00

abstract：:The oxidation of free methionine to methionine sulfoxide by chemically or enzymatically generated oxygen free radicals is presented. The physiological significance of this process in living cells is suggested. ...

journal_title：FEBS letters

authors： Scislowski PW,Davis EJ

更新日期：1987-11-16 00:00:00

abstract：:The metabolism of the double bonds at the delta 3 position in fatty acids was studied in rat liver. Infusion of delta 3-trans-dodecenoic acid into isolated perfused liver and subcellular fractionation studies showed the presence of both peroxisomal and mitochondrial delta 3,delta 2-enoyl-CoA isomerase activity (EC 5.3...

journal_title：FEBS letters

authors： Kärki T,Hakkola E,Hassinen IE,Hiltunen JK

更新日期：1987-05-11 00:00:00

abstract：:This study reports for the first time (a) the longitudinal profile of the transmembrane potential (mDeltapsi) of single mitochondria using a Nernstian fluorescent probe and (b) the distribution of mDeltapsi fluctuations of mitochondria undergoing permanent depolarization. Our findings show that (1) mitochondria in dif...

journal_title：FEBS letters

authors： Diaz G,Falchi AM,Gremo F,Isola R,Diana A

更新日期：2000-06-23 00:00:00

abstract：:Mesothelial cells, which cover the surface of visceral organs and serous cavities in mammals, play a crucial role in preventing adhesion. We previously reported that primary mesothelial progenitor cells (MPCs) can not only prevent postoperative adhesion but also promote liver regeneration after hepatectomy. Induced pl...

journal_title：FEBS letters

authors： Inagaki NF,Inagaki FF,Kokudo N,Miyajima A

更新日期：2019-02-01 00:00:00

abstract：:Interfering RNA was used to suppress the expression of the genes At1g06680 and At2g30790 in Arabidopsis thaliana, which encode the PsbP-1 and PsbP-2 proteins, respectively, of Photosystem II. A phenotypic series of transgenic plants was recovered that expressed intermediate and low amounts of PsbP. Earlier we had docu...

journal_title：FEBS letters

authors： Yi X,Hargett SR,Frankel LK,Bricker TM

更新日期：2009-06-18 00:00:00

abstract：:On incubation of 7-day-old wheat seedlings in the presence of [3H]thymidine, the radioactivity incorporated into coleoptile DNA is found to be localized mainly (greater than 95%) in the fraction of heavy mitochondrial DNA (H-mt DNA; rho = 1.716 gm/cm3). Upon long (48-72 h) incubation of cut-off seedlings in water, the...

journal_title：FEBS letters

authors： Kirnos MD,Alexandrushkina NI,Zagorskaya GYa,Kireev II,Vanyushin BF

更新日期：1992-02-24 00:00:00

abstract：:Cytochrome bd is a bacterial respiratory oxidase carrying three hemes but no copper. We show that nitric oxide (NO) reacts with the intermediate F of cytochrome bd from Azotobacter vinelandii: (i) with a 1:1 stoichiometry, (ii) rapidly (k=1.2 +/- 0.1 x 10(5)M(-1)s(-1) at 20 degrees C), and (iii) yielding the oxidized ...

journal_title：FEBS letters

authors： Borisov VB,Forte E,Sarti P,Brunori M,Konstantinov AA,Giuffrè A

更新日期：2006-09-04 00:00:00

abstract：:Human milk cholesterol esterase (bile salt-activated lipase) plays a role in the dietary uptake of triacylglyceride and cholesteryl ester. The activities toward these substrates are mediated through a unique bile salt-activated mechanism. Previously, it has been proposed that a necessary step in this process is prior ...

journal_title：FEBS letters

authors： Loomes KM,Senior HE

更新日期：1997-04-01 00:00:00

abstract：:cDNAs encoding aquaporins PIP1;1, PIP2;1, and TIP1;1 were isolated from Mimosa pudica (Mp) cDNA library. MpPIP1;1 exhibited no water channel activity; however, it facilitated the water channel activity of MpPIP2;1 in a phosphorylation-dependent manner. Mutagenesis analysis revealed that Ser-131 of MpPIP1;1 was phospho...

journal_title：FEBS letters

authors： Temmei Y,Uchida S,Hoshino D,Kanzawa N,Kuwahara M,Sasaki S,Tsuchiya T

更新日期：2005-08-15 00:00:00

abstract：:ATP synthesis rate, measured as the variation in external Pi concentration, varied as a linear function of either delta mu H+ or delta Gpin, in such a manner that the delta Gpin/delta mu H+ ratio increased while VATP increased. We also observed a linear dependence of the flux control coefficient of the Pi carrier on...

journal_title：FEBS letters

authors： Beauvoit B,Rigoulet M,Guerin B

更新日期：1989-02-27 00:00:00

abstract：:The metabolism of 20:4 (arachidonic acid) in alkenylacyl, alkylacyl and diacyl lipid classes in choline glycerophospholipids (CGP) and ethanolamine glycerophospholipids (EGP) in rabbit alveolar macrophages was examined. [3H]20:4 was very rapidly incorporated into diacyl glycerophosphocholine (GPC). After the removal o...

journal_title：FEBS letters

authors： Sugiura T,Katayama O,Fukui J,Nakagawa Y,Waku K

更新日期：1984-01-09 00:00:00

abstract：:Gene array profiling of RNA from cells engineered to express a dominant-negative version of the ETS family member transcription factor Pea3 (polyomavirus enhancer activator 3) identified WT1 as a candidate downstream gene. Given the co-expression of WT1 and Pea3 in developing kidney and breast tissue undergoing mesenc...

journal_title：FEBS letters

authors： Discenza MT,Vaz D,Hassell JA,Pelletier J

更新日期：2004-02-27 00:00:00

abstract：:Chronic indirect stimulation of fast twitch rabbit muscle induced a rapid reduction in parvalbumin concentration. When compared to the unstimulated contralateral muscle, parvalbumins were reduced to 55% following 6 days of stimulation. Prolonged stimulation further reduced parvalbumins so that they were undetectable a...

journal_title：FEBS letters

authors： Klug G,Reichmann H,Pette D

更新日期：1983-02-21 00:00:00

abstract：:TATA-box binding factor TFIID is one of the key factors in transcriptional activation. Surprisingly, the yeast TFDII protein [(1989) Nature 341, 299-303; (1989) Cell 56, 1173-1181; (1989) Proc. Natl. Acad. Sci USA 86, 7785-7789] reveals only limited homology with other DNA-binding proteins. From computer-assisted sear...

journal_title：FEBS letters

authors： Stucka R,Feldmann H

更新日期：1990-02-26 00:00:00

abstract：:Relative amounts of mRNA for cathepsin B were measured in normal murine liver and three murine tumors, an invasive liver tumor (hepatoma, Hepa cl 9) and two melanoma variants (B16-F1 and B16 amelanotic melanoma, B16a). Using a human cDNA to the cathepsin B coding region as a hybridization probe, we detected two specie...

journal_title：FEBS letters

authors： Moin K,Rozhin J,McKernan TB,Sanders VJ,Fong D,Honn KV,Sloane BF

更新日期：1989-02-13 00:00:00

abstract：:Expression of calcyclin in different cell lines and mouse tissues was determined with polyclonal antibodies raised against calcyclin from Ehrlich ascites tumour (EAT) cells. The protein was detected in mouse skeletal and cardiac muscle, in lung, kidney and spleen, and was especially enriched in mouse smooth muscle as ...

journal_title：FEBS letters

authors： Kuźnicki J,Filipek A,Heimann P,Kaczmarek L,Kamińska B

更新日期：1989-08-28 00:00:00

abstract：:A novel rapid polymerase chain reaction (PCR)-based technique for N-terminal attachment of green fluorescent protein (GFP) to a yeast protein is described. Genomic integration of a PCR-generated loxPkanMX4loxP-yEGFP fusion cassette immediately upstream of the open reading frame (ORF) allows for selection of G418 resis...

journal_title：FEBS letters

authors： Prein B,Natter K,Kohlwein SD

更新日期：2000-11-17 00:00:00

abstract：:Designed mutations within the Streptomyces lividans kcsA gene resulted in a set of mutant proteins, which were characterized in respect to their assembly and channel activities. (i) The amino acid residue leucine 81 located at the external side of KcsA was found to be exchangeable by a cysteine residue without affecti...

journal_title：FEBS letters

authors： Splitt H,Meuser D,Borovok I,Betzler M,Schrempf H

更新日期：2000-04-21 00:00:00

abstract：:The fluorescent Ca2+ indicator Fura 2 was used to monitor Ca2+ release induced by the Ins(1,4,5)P3-mobilizing agonist angiotensin II (Ag II), caffeine and 2,5-di-(tert-butyl)-1,4-benzohydroquinone (tuBHQ), in intact bovine adrenal chromaffin cells. Under low external Ca2+ conditions, tuBHQ, Ag II and caffeine elicited...

journal_title：FEBS letters

authors： Robinson IM,Burgoyne RD

更新日期：1991-09-09 00:00:00

abstract：:The relationship between the antioxidant effects of reduced coenzyme Q10 (ubiquinol, UQH2) and vitamin E (alpha-tocopherol) was investigated in beef heart submitochondrial particles in which lipid peroxidation was initiated by incubation with ascorbate + ADP-Fe3+. These effects were examined after extraction of coenzy...

journal_title：FEBS letters

authors： Forsmark P,Aberg F,Norling B,Nordenbrand K,Dallner G,Ernster L

更新日期：1991-07-08 00:00:00