Abstract:
:Expression of calcyclin in different cell lines and mouse tissues was determined with polyclonal antibodies raised against calcyclin from Ehrlich ascites tumour (EAT) cells. The protein was detected in mouse skeletal and cardiac muscle, in lung, kidney and spleen, and was especially enriched in mouse smooth muscle as well as in rat fibroblasts. No positive immunological reaction was detected in mouse brain, liver and intestine and some tumourigenic cell lines. The level of calcyclin mRNA found in different cells and tissues corresponded well to the calcyclin level estimated by immunoblotting. The calcyclin-like protein was purified from mouse stomach and appeared to be very similar to the EAT protein.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kuźnicki J,Filipek A,Heimann P,Kaczmarek L,Kamińska Bdoi
10.1016/0014-5793(89)81026-9subject
Has Abstractpub_date
1989-08-28 00:00:00pages
141-4issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(89)81026-9journal_volume
254pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The RNA helicase Rok1 plays a role in rRNA processing and in control of cell cycle progression in Saccharomyces cerevisiae. We identified two upstream open reading frames (uORFs) within the ROK1 5' untranslated region, which inhibited Rok1 translation. Mutating uATG to uAAG or generation of a premature stop codon in t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.10.019
更新日期:2010-11-19 00:00:00
abstract::Protein synthesis in cell-free systems of rat liver and kidney decreases markedly with age. Examination of activity changes of the different steps revealed for both types of organs that reduced binding of aminoacyl-tRNA to ribosomes and reduced peptidyl transfer might be of major importance for the decrease in overall...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80948-x
更新日期:1983-08-22 00:00:00
abstract::Differences in the metabolism of cancer cells or cancer stem cells (CSCs) as compared to normal cells have provided avenues to safely target cancers. To discover metabolic inhibitors of CSCs, we performed alkaline phosphatase- and tumoursphere-based drug screening using induced cancer stem cell-like cells. From the sc...
journal_title:FEBS letters
pub_type: 社论
doi:10.1002/1873-3468.13361
更新日期:2019-04-01 00:00:00
abstract::To determine the amino acid residues required for the signal-transducing activity of the human c-Ha-Ras protein, we introduced point mutations at residues 45-54 near the 'effector region' (residues 32-40). We transfected PC12 cells with these mutant genes and also micro-injected the mutant proteins, bound with an unhy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80665-p
更新日期:1991-12-09 00:00:00
abstract::Transport of the precursor for carbamoyl-phosphate synthetase I into mitochondria in Reuber hepatoma H-35 cells was inhibited by adding monensin or nigericin to the culture medium at a concentration of 0.5 microM, and the enzyme precursor accumulated, mainly in the cytosolic fraction. Accumulated precursor was degrade...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80029-0
更新日期:1984-01-02 00:00:00
abstract::The structure of [Val1]gramicidin A incorporated into sodium dodecyl-d25 sulphate micelles has been studied by two-dimensional proton NMR spectroscopy. Analysis of nuclear Overhauser effects, spin-spin couplings and solvent accessibility of NH groups show that the conformation of the Na+ complex of gramicidin A in det...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80702-x
更新日期:1985-07-08 00:00:00
abstract::The use of tobacco plants as a novel expression system for the production of human homotrimeric collagen I is presented in this report. Constructs were engineered from cDNA encoding the human proalpha1(I) chain to generate transgenic tobacco plants expressing collagen I. The recombinant proalpha1(I) chains were expres...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01259-x
更新日期:2000-03-03 00:00:00
abstract::The role of protein kinase C (PKC) and p42(MAP kinase) signaling in the regulation of proliferation and apoptosis was investigated in freshly isolated and primary cultured rat hepatocytes. Acute treatment of freshly isolated hepatocytes with phenylephrine and EGF caused rapid phasic activations of p42(MAP kinase) and ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00705-9
更新日期:1997-07-21 00:00:00
abstract::In PC12 cells, cAMP stimulates the MAP kinase pathway by an unknown mechanism. Firstly, we examined the role of calcium ion mobilization and of protein kinase C in cAMP-stimulated MAP kinase activation. We show that cAMP stimulates p44mapk independently of these events. Secondly, we studied the role of B-Raf in this p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01376-c
更新日期:1995-01-09 00:00:00
abstract::The possible in vivo interaction of the Nicotiana tabacum agglutinin (Nictaba) with endogenous glycoproteins was corroborated using a combination of confocal/electron microscopy of an EGFP-Nictaba fusion protein expressed in tobacco Bright Yellow-2 (BY-2) cells and biochemical analyses. In vitro binding studies demons...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.10.044
更新日期:2006-11-27 00:00:00
abstract::Cloned mouse delta-subtype opioid receptor (DOR1) was expressed in Xenopus oocytes to study the signal transduction. Opioid delta-agonists evoked a calcium-dependent chloride current in oocytes injected with mRNA derived from DOR1, together with that from the alpha subunit of Gi1. The delta-agonist-induced current was...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80677-m
更新日期:1993-11-01 00:00:00
abstract::Antisera were raised against a phosphoprotein of 65 kDa (PP65) from Paramecium cells (shown before to be selectively dephosphorylated during synchronous exocytosis) and specified by immunoblotting. By immunofluorescence PP65 has been localized within the cortex, beneath the cell membrane. This corresponds to data obta...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80503-3
更新日期:1987-10-19 00:00:00
abstract::Hydrin 2 (vasotocinyl-Gly), a hydroosmotic peptide resulting from differential processing of provasotocin and recently identified in frog neurohypophysis, has been looked for in the pituitary gland of an exotic toad (Bufo marinus) and of a reptile (Vipera aspis). Hydrin 2 has been found in the amphibian but not in the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80783-f
更新日期:1990-05-07 00:00:00
abstract::The secretion mechanisms of cathepsin L from osteoclasts in the process of bone resorption were investigated. The increases in bone pit numbers formed take place by PTH addition in parallel with the increases of cathepsin L and/or L-like proteinase activities in the culture medium of bone cells, and these were suppres...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80522-9
更新日期:1994-04-11 00:00:00
abstract::Spermatid-specific thioredoxin-1 (Sptrx-1) is the first member of the thioredoxin family of proteins with a tissue-specific expression pattern, found exclusively in the tail of elongating spermatids and spermatozoa. We describe here further biochemical characterization of human Sptrx-1 protein structure and enzymatic ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03417-8
更新日期:2002-10-23 00:00:00
abstract::Electrospray/mass spectrometry (ES/MS) was extensively used to obtain information on disulphide-containing intermediates formed during refolding of bovine pancreatic ribonuclease A. The analysis showed the existence of an equilibrated population of disulphide bonded intermediates, and indicates that intermediates cont...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00966-x
更新日期:1994-10-03 00:00:00
abstract::The mitochondrial dynamin-like GTPase Mgm1 exists as a long (l-Mgm1) and a short isoform (s-Mgm1). They both are essential for mitochondrial fusion. Here we show that the isoforms interact in a homotypic and heterotypic manner. Their submitochondrial distribution between inner boundary membrane and cristae was markedl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.05.053
更新日期:2009-07-07 00:00:00
abstract::In eukaryotic cells, peroxiredoxins are both antioxidants and regulators of H(2)O(2)-mediated signaling. We previously found that peroxiredoxin-I (Prx-I) was overexpressed in non-small cell lung cancer (NSCLC) tissue. Since overexpressed protein can induce a humoral immune response, we examined whether serum from NSCL...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.04.028
更新日期:2005-05-23 00:00:00
abstract::The crystal structure for cce_0566 (171 aa, 19.4 kDa), a DUF269 annotated protein from the diazotrophic cyanobacterium Cyanothece sp. ATCC 51142, was determined to 1.60Å resolution. Cce_0566 is a homodimer with each molecule composed of eight α-helices folded on one side of a three strand anti-parallel β-sheet. Hydrop...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.01.037
更新日期:2012-02-17 00:00:00
abstract::This study reports plasma levels of a specific nonenzymatic peroxidation product of arachidonic acid, esterified 8-epi-PGF2 alpha, from healthy- and NIDDM individuals as an index of oxidative stress in vivo. Plasma 8-epi-PGF2 alpha was isolated by solid-phase extraction on a C18 followed by an NH2 cartridge and analyz...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00649-t
更新日期:1995-07-17 00:00:00
abstract::Reticulocyte-type 15-lipoxygenase is known to dioxygenate phospholipids without preceding action of phospholipases A2 (PLA2). Therefore we studied the reaction of the secretory PLA2s (sPLA2) from pancreas and snake venom, and of the human cytosolic PLA2 (cPLA2) with 1-palmitoyl-2-arachidonoyl phosphatidylcholine (PAPC...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01024-2
更新日期:1998-09-04 00:00:00
abstract::In human pancreatic islets an increase in the glucose concentration from 3 to 20 mM raised the free cytoplasmic Ca2+ concentration [( Ca2+]i), an effect being reversible upon withdrawal of the sugar. Depolarization with a high concentration of K+ or the sulphonylurea tolbutamide also raised [Ca2+]i. Addition of extrac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81309-v
更新日期:1991-10-21 00:00:00
abstract::Amylin, a 37 amino acid C-terminal amidated peptide is an integral part of secretory granules of pancreatic beta-cells. Utilizing a specific radioimmunoassay system we demonstrate in the present study a cosecretion of amylin and insulin from the isolated rat pancreas. The secretion pattern of both peptides during gluc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80210-a
更新日期:1990-03-26 00:00:00
abstract::Molecular dynamics analyses were performed to examine conformational changes in the C-domain of calmodulin and the N-domain of troponin C induced by binding of Ca(2+) ions. Analyses of conformational changes in calmodulin and troponin C indicated that the shortening of the distance between Ca(2+) ions and Ca(2+) bindi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00114-0
更新日期:2004-03-12 00:00:00
abstract::Oligonucleotides 5'-d(CT)5-L-d(AG)5-L-d(GA)5-3' and 5'-d(GA)5-L-d(TC)5-L-d(GA)5-3' [L = pO(CH2CH2O)3p] were studied by thermal denaturation, chemical modification and binding of fluorescent dyes. Both oligonucleotides are shown to fold back on itself twice forming at pH 7 a sufficiently stable triplex ether with antip...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00519-f
更新日期:1995-06-19 00:00:00
abstract::Human aminopeptidase N carries an apical sorting signal on its ectodomain necessary for its correct transport to the apical membrane in Madin-Darby canine kidney cells. To determine whether the apical sorting signal is localized in the serine/threonine rich stalk or in the catalytic head group, anchor/stalk-minus amin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81039-o
更新日期:1992-08-10 00:00:00
abstract::A specific b subunit arginine, b(Arg-36) in Escherichia coli, displays evolutionary conservation among bacterial F1F0 ATP synthases. Site-directed mutagenesis was used to generate a collection of mutations affecting b(Arg-36). The phenotype differed depending upon the substitution, and the b(Arg-36-Glu) and b(Arg-36-I...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00597-3
更新日期:1998-06-12 00:00:00
abstract::After initial purification of ovine placental lactogen (oPL) using the procedures described previously [(1976) Endocrinology 98, 65-75], the oPL preparation was further purified by high-performance liquid chromatography (HPLC) using an anionic exchange column (Bio-Sil TSK DEAE-2-SW). Two forms of oPL with different re...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80491-4
更新日期:1986-04-21 00:00:00
abstract::Retroviral proteinases (PRs) are essential for retrovirus infectivity but the mechanism of their activity regulation is poorly understood. We investigated possible involvement in this process of the C-terminal domain (CTD) of betaretroviral PRs. We found that the presence of CTD attenuates proteolytic activity of Maso...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.09.010
更新日期:2004-10-08 00:00:00
abstract::We have recently developed a method to produce native human proinsulin using a bacterial expression system. A proinsulin fusion protein was recovered from inclusion bodies and cleaved using cyanogen bromide. The released proinsulin polypeptide was S-sulfonated and purified by anion exchange chromatography. Following r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01511-6
更新日期:1997-02-03 00:00:00