Abstract:
:Electrospray/mass spectrometry (ES/MS) was extensively used to obtain information on disulphide-containing intermediates formed during refolding of bovine pancreatic ribonuclease A. The analysis showed the existence of an equilibrated population of disulphide bonded intermediates, and indicates that intermediates containing two intramolecular S-S are predominant until late stages of the refolding process. Mixed disulphides with exogenous glutathione were also detected, supporting previous evidence of conformational restrictions on the ability of RNase A to form intramolecular disulphides. The results indicate that ES/MS is a suitable technique to detect and characterize refolding intermediates.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Torella C,Ruoppolo M,Marino G,Pucci Pdoi
10.1016/0014-5793(94)00966-xsubject
Has Abstractpub_date
1994-10-03 00:00:00pages
301-6issue
3eissn
0014-5793issn
1873-3468journal_volume
352pub_type
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