Expression, purification and characterization of recombinant human proinsulin.

abstract：:Telomerase is a multimolecular complex of reverse transcriptase, RNA template, and regulatory proteins. It has two known functions: catalysis of the addition of [TTAGGG] repeats to telomeric DNA and the activation of various genes controlling cell proliferation. The possible coordination of these two functions is a ke...

journal_title：FEBS letters

authors： Wolfrom C,Martin OC,Laurent M,Deschatrette J

更新日期：2007-01-09 00:00:00

abstract：:Extracts of the alkaliphilic sulfur-oxidizing autotroph strain AL3 contained sulfide:cytochrome c oxidoreductase. This was active above pH 8, and was associated with the cell membranes. Although up to 60% of the initial activity was lost during Triton X-100 extraction, further purification resulted in an enzyme that c...

journal_title：FEBS letters

authors： Sorokin DYu,de Jong GA,Robertson LA,Kuenen GJ

更新日期：1998-05-01 00:00:00

abstract：:Calmodulin is shown to interact with human spectrin dimer. The binding was highly calcium-dependent and observed in two different kinds of experiments. Firstly, affinity chromatography of calmodulin on a Sepharose 4B column with immobilized spectrin, and secondly, partition in aqueous two-phase polymer systems. In the...

journal_title：FEBS letters

authors： Berglund A,Backman L,Shanbhag VP

更新日期：1984-06-25 00:00:00

abstract：:We introduced rat ornithine transcarbamylase (OTC) gene into OTC-deficient spf-ash mice by mating spf-ash heterozygotes with transgenic mice which carried recombinant DNA composed of 1.3 kb of the 5' flanking region of the gene fused onto rat OTC cDNA. The liver OTC activity of hemizygous spf-ash mice which carried th...

journal_title：FEBS letters

authors： Shimada T,Noda T,Tashiro M,Murakami T,Takiguchi M,Mori M,Yamamura K,Saheki T

更新日期：1991-02-25 00:00:00

abstract：:A site-selective cAMP analog, 8-chloroadenosine 3',5'-cyclic monophosphate (8-Cl-cAMP), was demonstrated to be a potent inhibitor of both the monolayer and soft agar growth of normal rat kidney (NRK) fibroblasts that had been transformed with the v-Ki-ras oncogene or treated with transforming growth factor alpha (TGF ...

journal_title：FEBS letters

authors： Tortora G,Ciardiello F,Ally S,Clair T,Salomon DS,Cho-Chung YS

更新日期：1989-01-02 00:00:00

abstract：:A catalytic sequence motif PDX10-30(E/D)XK is found in many restriction enzymes. On the basis of sequence similarities and mapping of the conserved residues to the crystal structure of NgoMIV we suggest that residues D160, K182, R186, R188 and E195 contribute to the catalytic/DNA binding site of the Ecl18kI restrictio...

journal_title：FEBS letters

authors： Tamulaitis G,Solonin AS,Siksnys V

更新日期：2002-05-08 00:00:00

abstract：:The ubiquitin-like protein Urm1 from budding yeast and its E1-like activator Uba4 have dual roles in protein urmylation and tRNA thiolation pathways. To study whether these are conserved among eukaryotes, we used gene shuffles to replace the yeast proteins by their human counterparts, hURM1 and hUBA4/MOCS3. As judged ...

journal_title：FEBS letters

authors： Jüdes A,Ebert F,Bär C,Thüring KL,Harrer A,Klassen R,Helm M,Stark MJ,Schaffrath R

更新日期：2015-04-02 00:00:00

abstract：:Barley thioredoxin h isozymes 1 (HvTrxh1) and barley thioredoxin h isozymes 2 (HvTrxh2) show distinct spatiotemporal distribution in germinating seeds. Using a novel approach involving measurement of bidirectional electron transfer rates between Escherichia coli thioredoxin, which exhibits redox-dependent fluorescence...

journal_title：FEBS letters

authors： Maeda K,Hägglund P,Björnberg O,Winther JR,Svensson B

更新日期：2010-08-04 00:00:00

abstract：:Trypanosomes are unicellular parasites and like all decent parasites, they try to obtain from the host as much material as possible, including lipids. However, the needs of a parasite are not always the same as those of the host, and therefore, mostly, some biosynthetic work still has to be done by the parasite itself...

journal_title：FEBS letters

authors： van Hellemond JJ,Tielens AG

更新日期：2006-10-09 00:00:00

abstract：:The assembly of iron-sulfur (Fe/S) clusters in a living cell is mediated by a complex machinery which, in eukaryotes, is localised within mitochondria. Here, we report on a new component of this machinery, the protein Isa2p of the yeast Saccharomyces cerevisiae. The protein shares sequence similarity with yeast Isa1p ...

journal_title：FEBS letters

authors： Pelzer W,Mühlenhoff U,Diekert K,Siegmund K,Kispal G,Lill R

更新日期：2000-07-07 00:00:00

abstract：:In this work, we examined the antioxidant role of mucin, a typical sialic acid containing high-molecular weight glycoprotein. The function of mucin as a hydroxyl radical (.OH) scavenger was characterized using bovine submaxillary gland mucin (BSM). Non-treated BSM effectively protected DNA from the attack of .OH; howe...

journal_title：FEBS letters

authors： Ogasawara Y,Namai T,Yoshino F,Lee MC,Ishii K

更新日期：2007-05-29 00:00:00

abstract：:In humans, many responses to hypoxia including angiogenesis and erythropoiesis are mediated by the alpha/beta-heterodimeric transcription factor hypoxia inducible factor (HIF). The stability and/or activity of human HIF-1alpha are modulated by post-translational modifications including prolyl and asparaginyl hydroxyla...

journal_title：FEBS letters

authors： Murray-Rust TA,Oldham NJ,Hewitson KS,Schofield CJ

更新日期：2006-04-03 00:00:00

abstract：:The F-box protein Skp2 is important for S phase entry and binds to Skp1 and the cyclin A-Cdk2 complex. Here we report the cloning, analysis of genomic organization and characterization of a novel gene product related to Skp2 named FBL2. The human FBL2 gene was found to be a highly interrupted gene of at least 126.6 kb...

journal_title：FEBS letters

authors： Ilyin GP,Rialland M,Glaise D,Guguen-Guillouzo C

更新日期：1999-10-01 00:00:00

abstract：:In Alzheimer's disease and related dementias, human tau protein aggregates into paired helical filaments and neurofibrillary tangles. However, such tau aggregates have not yet been demonstrated in transgenic mouse models of the disease. One of the possible explanations would be that mouse tau has different properties ...

journal_title：FEBS letters

authors： Kampers T,Pangalos M,Geerts H,Wiech H,Mandelkow E

更新日期：1999-05-14 00:00:00

abstract：:Caloric restriction (CR) is known to promote longevity in various species. Sirtuin-mediated deacetylation has been shown to be related to the promotion of longevity in some species. Here, we show that CR of rats led to an increase in the level of Werner syndrome protein (WRN), a recognized DNA repair protein. In addit...

journal_title：FEBS letters

authors： Kahyo T,Mostoslavsky R,Goto M,Setou M

更新日期：2008-07-23 00:00:00

abstract：:Niemann-pick type C1 (NPC1) is an endo/lysosomal membrane protein involved in intracellular cholesterol trafficking, and its luminal domain C is an essential endosomal receptor for Ebola and Marburg viruses. We have determined the crystal structure of glycosylated NPC1 luminal domain C and find all seven possible site...

journal_title：FEBS letters

authors： Zhao Y,Ren J,Harlos K,Stuart DI

更新日期：2016-03-01 00:00:00

abstract：:Over 400 human proteases documented in secondary databases can already be delineated in genomic sequence. A Genome Ontology annotation of 30585 sequences in the provisional human proteome set recognises 498 proteases, i.e. 1.6%. Homology searches against finished sequence and comparisons between mouse and zebrafish ar...

journal_title：FEBS letters

authors： Southan C

更新日期：2001-06-08 00:00:00

abstract：:We examined the expression of peroxisome proliferator-activated receptors (PPARs) and the role of PPARs in cytokine production in mouse bone marrow-derived mast cells (mBMMCs). mBMMCs expressed PPARbeta strongly and gamma slightly, but not alpha. Activation of mBMMCs with antigen or calcium ionophore resulted in the i...

journal_title：FEBS letters

authors： Sugiyama H,Nonaka T,Kishimoto T,Komoriya K,Tsuji K,Nakahata T

更新日期：2000-02-11 00:00:00

abstract：:Incubation of phosphatidylethanolamine containing liposomes with malondialdehyde and other aldehydes with different chain lengths results in the fluorescence of chromolipids. Relative to malondialdehyde, the fluorescence was greatly enhanced with increasing chain length upon incubation of 2-alkenals with phospholipids...

journal_title：FEBS letters

authors： Montfoort A,Bezstarosti K,Groh MM,Koster JF

更新日期：1987-12-21 00:00:00

abstract：:Vesicular monoamine transporters (VMAT) catalyze transport of serotonin, dopamine, epinephrine and norepinephrine into subcellular storage organelles in a variety of cells. Accumulation of the neurotransmitter depends on the proton electrochemical gradient across the organelle membrane and involves VMAT-mediated excha...

journal_title：FEBS letters

authors： Shirvan A,Laskar O,Steiner-Mordoch S,Schuldiner S

更新日期：1994-12-12 00:00:00

abstract：:Free radicals, including dopamine (DA)-oxidized metabolites, have long been implicated in pathogenesis of Parkinson's disease (PD). However, the relationships between such oxidative stresses and alpha-synuclein (alpha-S), a major constituent of Lewy bodies, remain unknown. In this study, we established neuronal cells ...

journal_title：FEBS letters

authors： Hasegawa T,Matsuzaki-Kobayashi M,Takeda A,Sugeno N,Kikuchi A,Furukawa K,Perry G,Smith MA,Itoyama Y

更新日期：2006-04-03 00:00:00

abstract：:Here we show that increased amount of secondary structure is acquired in the folded states of two structurally-different proteins (alpha-helical VlsE and alpha/beta flavodoxin) in the presence of macromolecular crowding agents. The structural content of flavodoxin and VlsE is enhanced by 33% and 70%, respectively, in ...

journal_title：FEBS letters

authors： Perham M,Stagg L,Wittung-Stafshede P

更新日期：2007-10-30 00:00:00

abstract：:Polymyxin B (PXB), a cyclic peptide antibiotic, in concentrations 0.1-3.0 mg/ml (0.08-4.0 mmol/l), inhibited the K+ efflux induced by opening of the Ca2+-activated K+ channel (the Gárdos effect) in intact human red blood cells. The inhibition was observed when the Gárdos effect was elicited by Ca2+ in the presence of ...

journal_title：FEBS letters

authors： Varecka L,Peterajová E,Pogády J

更新日期：1987-12-10 00:00:00

abstract：:Nitrate compartmentalization in intracellular organelles has been long recognized as critical for plant physiology but the molecular identity of the proteins involved remained unclear for a long time. In Arabidopsis thaliana, AtClC-a has been recently shown to be a NO(3)(-)/H(+) antiporter critical for nitrate transpo...

journal_title：FEBS letters

authors： Zifarelli G,Pusch M

更新日期：2010-05-17 00:00:00

abstract：:In brain tissue a spectrin-like calmodulin-binding protein calspectin, or fodrin, is concentrated in a synaptosome fraction, where most of the calspectin is associated with the synaptic membranes. This endogenous calspectin was phosphorylated by protein kinase system(s) associated with the membranes. Here, we report t...

journal_title：FEBS letters

authors： Sobue K,Kanda K,Kakiuchi S

更新日期：1982-12-13 00:00:00

abstract：:In mouse mammary epithelial cells in primary culture, mechanical stimulation of a cell induced in other cells within the same colony a short depolarization of less than 15 mV with a duration of 1-8 s and a subsequent, prominent hyperpolarization of 6 mV lasting 10-40 s. Epidermal growth factor induces a spontaneous hy...

journal_title：FEBS letters

authors： Enomoto K,Furuya K,Maeno T,Edwards C,Oka T

更新日期：1987-10-19 00:00:00

abstract：:Novel catechin derivatives obtained from grape procyanidins and l-cysteine scavenge free radicals by hydrogen atom donation, rather than electron transfer, and reduce cell viability in A375 and M21 melanoma cells. In particular, 4beta-(S-cysteinyl)epicatechin 3-O-gallate has a free radical scavenging capacity as stron...

journal_title：FEBS letters

authors： Lozano C,Torres JL,Julià L,Jimenez A,Centelles JJ,Cascante M

更新日期：2005-08-15 00:00:00

abstract：:Heme is essential and synthesized via highly regulated processes. For this reason, most organisms strive to recycle it or acquire it from their environment. When heme is bound to proteins noncovalently, degradation of the polypeptide is sufficient to release it. However, in some hemoproteins, such as c-type cytochrome...

journal_title：FEBS letters

authors： Murphey AC,Mavridou DAI,Hodgkin J,Ferguson SJ

更新日期：2018-03-01 00:00:00

abstract：:Vacuolar H(+)-PPase, a membrane bound proton-translocating pyrophosphatase found in various species including plants, some protozoan and prokaryotes, has been demonstrated to be localized to the vacuolar membrane in plants. Using a GUS reporter system and a green fluorescent protein (GFP) fusion protein, we investigat...

journal_title：FEBS letters

authors： Mitsuda N,Enami K,Nakata M,Takeyasu K,Sato MH

更新日期：2001-01-12 00:00:00

abstract：:Plasma citrate levels were found to be elevated in non-alcoholic fatty liver disease (NAFLD) patients. Cellular experiments indicated that increased citrate levels might originate from an excess of fatty acids. The impact of elevated citrate levels on oxidative stress was examined. It was found that citrate stimulated...

journal_title：FEBS letters

authors： van de Wier B,Balk JM,Haenen GR,Giamouridis D,Bakker JA,Bast BC,den Hartog GJ,Koek GH,Bast A

更新日期：2013-08-02 00:00:00