Abstract:
:Reticulocyte-type 15-lipoxygenase is known to dioxygenate phospholipids without preceding action of phospholipases A2 (PLA2). Therefore we studied the reaction of the secretory PLA2s (sPLA2) from pancreas and snake venom, and of the human cytosolic PLA2 (cPLA2) with 1-palmitoyl-2-arachidonoyl phosphatidylcholine (PAPC) and their 15-lipoxygenated species (PAPC-OOH and PAPC-OH) either alone or as equimolar mixtures. These PLA2s cleaved PAPC-O(O)H with higher (sPLA2) or similar rates (cPLA2) as compared with native PAPC. In mixtures, however, PAPC proved to be the preferred, albeit not exclusive substrate for all three PLA2s. Thus, partial 15-lipoxygenation of phospholipids may also trigger liberation of arachidonic acid.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Chaitidis P,Schewe T,Sutherland M,Kühn H,Nigam Sdoi
10.1016/s0014-5793(98)01024-2subject
Has Abstractpub_date
1998-09-04 00:00:00pages
437-41issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(98)01024-2journal_volume
434pub_type
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