Solution NMR determination of hydrogen bonding and base pairing between the glyQS T box riboswitch Specifier domain and the anticodon loop of tRNA(Gly).

abstract：:Clusters of codons pairing to low-abundance tRNAs synchronize the translation with co-translational folding of single domains in multidomain proteins. Although proven with some examples, the impact of the ribosomal speed on the folding and solubility on a global, cell-wide level remains elusive. Here we show that upre...

journal_title：FEBS letters

authors： Fedyunin I,Lehnhardt L,Böhmer N,Kaufmann P,Zhang G,Ignatova Z

更新日期：2012-09-21 00:00:00

abstract：:Investigations of the cleavage specificity of a cyclizing endoribonuclease of yeast show that it hydrolyzes Y-A bonds of single-stranded regions of RNA and that oligonucleotides are hydrolyzed poorly. The stringent specificity of the enzyme suggests that it may be useful for generating specific fragments of polyribonu...

journal_title：FEBS letters

authors： Stevens A

更新日期：1986-09-15 00:00:00

abstract：:Hypo-osmotic swelling of human Intestine 407 cells leads to a significant increase of intracellular MAPKAP-kinase 2 activity and Hsp27 phosphorylation. Pre-treatment of the cells with the p38 MAP kinase inhibitor SB-203580 blocks this activation, indicating that the hypotonicity-induced activation of MAPKAP kinase 2 i...

journal_title：FEBS letters

authors： Tilly BC,Gaestel M,Engel K,Edixhoven MJ,de Jonge HR

更新日期：1996-10-21 00:00:00

abstract：:Quantitative structure activity relationships (QSARs) for the conversion of nitrobenzimidazolones and nitrobenzimidazoles by rat liver DT-diaphorase (EC 1.6.99.2) are described. The parameter used for description of the QSARs is the energy of the lowest unoccupied molecular orbital (E(LUMO)) of the nitroaromatic compo...

journal_title：FEBS letters

authors： Anusevicius Z,Soffers AE,Cénas N,Sarlauskas J,Segura-Aguilar J,Rietjens IM

更新日期：1998-05-15 00:00:00

abstract：:Replacements of Asp-295 by Asn (D295N) and Glu (D295E) decreased the catalytic center activity of Leuconostoc mesenteroides sucrose phosphorylase to about 0.01% of the wild-type level (k(cat)=200s(-1)). Glucosylation and deglucosylation steps of D295N were affected uniformly, approximately 10(4.3)-fold, and independen...

journal_title：FEBS letters

authors： Mueller M,Nidetzky B

更新日期：2007-04-03 00:00:00

abstract：:In human platelet membranes enhanced tyrosine phosphorylation of certain proteins was observed when Zn2+ instead of Mg2+ or Mn2+ was used as a divalent cation for the kinase reaction. An enhanced level of phosphate incorporation into tyrosine residues occurred into a 68 kDa polypeptide besides the 45 kDa and 105 kDa p...

journal_title：FEBS letters

authors： Findik D,Presek P

更新日期：1988-08-01 00:00:00

abstract：:Apoptosis is a physiological process for active cell removal. One of its hallmarks is an increased cytosolic Ca2+ content. Several genes involved in apoptosis control have been identified, but their mode of action is not understood in detail. Apoptosis may relate to oncogenesis, in that some malignant tumors may grow ...

journal_title：FEBS letters

authors： Richter C

更新日期：1993-06-28 00:00:00

abstract：:Ghrelin is a novel gastrointestinal peptide that stimulates growth hormone secretion, food intake, and body weight gain. Increased ghrelin secretion has been reported in such negative energy states as starvation and low body weight. We investigated the dynamics of ghrelin in rats with streptozotocin-induced diabetes, ...

journal_title：FEBS letters

authors： Masaoka T,Suzuki H,Hosoda H,Ota T,Minegishi Y,Nagata H,Kangawa K,Ishii H

更新日期：2003-04-24 00:00:00

abstract：:A subcellular fraction, highly enriched in uncoated vesicles (UCV) with high H+-ATPase (EC 3.6.1.34) activity, was isolated from the crude microsomal fraction of rat liver homogenates by discontinuous sucrose gradient centrifugation. The UCV fraction, recovered at the interface of sucrose density 1.08 and 1.10 g/ml, w...

journal_title：FEBS letters

authors： Flatmark T,Haavik J,Grønberg M,Jorunn Kleiveland L,Wahlstrøm Jacobsen S,Berge SV

更新日期：1985-09-02 00:00:00

abstract：:Here, we used a reductant, N-acetyl-L-cysteine (NAC), to investigate the redox-sensitive step(s) in the signalling pathway from the tumor necrosis factor (TNF) receptor to nuclear factor kappaB (NF-kappaB). We found that NAC suppressed NF-kappaB activation triggered by TNF or by overexpression of either the TNF recept...

journal_title：FEBS letters

authors： Oka S,Kamata H,Kamata K,Yagisawa H,Hirata H

更新日期：2000-04-28 00:00:00

abstract：:The von Hippel-Lindau (VHL) tumor suppressor gene regulates the extracellular matrix by controlling fibronectin deposition. To identify novel VHL target genes, we subjected mRNA from VHL-deficient RCC cells (786-0-pRC) and a transfectant re-expressing wildtype VHL (786-0-VHL) to differential expression profiling. Amon...

journal_title：FEBS letters

authors： Bluyssen HA,Lolkema MP,van Beest M,Boone M,Snijckers CM,Los M,Gebbink MF,Braam B,Holstege FC,Giles RH,Voest EE

更新日期：2004-01-02 00:00:00

abstract：:Internalization of membrane proteins has been studied for more than three decades without solving all the underlying mechanisms. Our knowledge of the clathrin-coated endocytosis is sufficient to understand the basic principles. However, more detailed insight is required to recognize why different proteins enter clathr...

journal_title：FEBS letters

authors： Strous GJ,Gent J

更新日期：2002-10-02 00:00:00

abstract：:In the brain inwardly rectifying potassium channel Kir7.1 subunits are predominantly expressed in the choroid plexus and meninges. To investigate this tissue-specific expression pattern, we characterized the genomic organization and the 5' proximal promoter of the rat Kir7.1 gene (Kcnj13). Starting from the major tran...

journal_title：FEBS letters

authors： Döring F,Karschin A

更新日期：2000-10-20 00:00:00

abstract：:Cerebellar granule cells differentiated in culture were incubated with ganglioside [3H-Sph]GM1 in order to have it inserted into the plasma membrane and metabolized. Among the formed metabolites radioactive sphingosine and ceramide were identified. [3H]Ceramide started to be measurable after 10 min of incubation (puls...

journal_title：FEBS letters

authors： Riboni L,Bassi R,Sonnino S,Tettamanti G

更新日期：1992-03-30 00:00:00

abstract：:Antipsychotic drugs of known antidiarrhoeal and anticalmodulin activity inhibited the cholera-toxin-catalysed ADP-ribosylation of proteins of Mr 37,000, 40,000 and 45,000 (thought to be regulatory components of the adenylate cyclase complex) that was previously shown to occur in plasma membranes from rabbit intestinal...

journal_title：FEBS letters

authors： Longbottom D,van Heyningen S

更新日期：1990-10-15 00:00:00

abstract：:Two major embryonic myosin heavy chains are expressed in embryonic chick breast muscle until the first week after hatching. Of these, one is already detected in the 8-day-old embryo. The other appears on day 12. Two putative slow embryonic isoforms represent minor components transiently expressed between days 8 and 12...

journal_title：FEBS letters

authors： Hofmann S,Düsterhöft S,Pette D

更新日期：1988-10-10 00:00:00

abstract：:The temperate phage phi H of the extremely halophilic archaebacterium Halobacterium salinarium encodes a repressor, Rep, which in the immune state represses the production of an early lytic transcript, denoted T4. Rep acts at the transcriptional level by blocking the promoter for T4. The promoter for the rep gene itse...

journal_title：FEBS letters

authors： Stolt P,Zillig W

更新日期：1994-05-16 00:00:00

abstract：:The present one-drug-one-target paradigm in drug discovery has been considered partially responsible for the more-funding-less-drug predicament in modern pharmaceutical industry. To hit the multiple targets implicated in complex diseases, two strategies, based on multicomponent or single-ingredient, are conceivable. A...

journal_title：FEBS letters

authors： Zhang HY

更新日期：2005-10-10 00:00:00

abstract：:The angiotensin II type I (AT(1)) receptor mediates regulation of blood pressure and water-electrolyte balance by Ang II. Substitution of Gly for Asn(111) of the AT(1) receptor constitutively activates the receptor leading to Gq-coupled IP(3) production independent of Ang II binding. The Ang II-activated conformation ...

journal_title：FEBS letters

authors： Lee C,Hwang SA,Jang SH,Chung HS,Bhat MB,Karnik SS

更新日期：2007-05-29 00:00:00

abstract：:The octapeptide Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr (peptide T) and two structural analogs are potent agonists of human monocyte chemotaxis, evincing identical rank potency orders as was previously shown for their inhibition of human immunodeficiency virus (HIV) envelope binding and T cell infectivity. Chemotactic activit...

journal_title：FEBS letters

authors： Ruff MR,Martin BM,Ginns EI,Farrar WL,Pert CB

更新日期：1987-01-19 00:00:00

abstract：:The precise interactions between the subunits of Gs (alpha s, beta, gamma) and the plasma membrane remain to be established. If alpha s is associated loosely with the inner membrane, is labile during activation, or is always present to some extent in the cytoplasm, then it should fractionate to the supernatant of a hi...

journal_title：FEBS letters

authors： Roth DA,Urasawa K,Leiber D,Insel PA,Hammond HK

更新日期：1992-01-13 00:00:00

abstract：:Dark-grown angiosperm seedlings lack chlorophylls, but accumulate protochlorophyllide a complexed with the light-dependent enzyme NADPH:protochlorophyllide oxidoreductase. Previous investigators correlated spectral heterogeneity of in vivo protochlorophyllide forms and a shift of chlorophyllide forms from 680 to 672 n...

journal_title：FEBS letters

authors： Klement H,Oster U,Rüdiger W

更新日期：2000-09-01 00:00:00

abstract：:Researchers still have great difficulty in isolating individual ribosomal proteins from the ribosome in quantities high enough for structural research. To this end, when studying protein S7, we created an E. coli overproducer of the recombinant protein S7 of Thermus thermophilus. The vector for expression was pQE-32 h...

journal_title：FEBS letters

authors： Karginov AV,Karginova OA,Spiridonova VA,Kopylov AM

更新日期：1995-08-07 00:00:00

abstract：:Gap junctions (GJs) traverse apposing membranes of neighboring cells to mediate intercellular communication by passive diffusion of signaling molecules. We have shown previously that cells endocytose GJs utilizing the clathrin machinery. Endocytosis generates cytoplasmic double-membrane vesicles termed annular gap jun...

journal_title：FEBS letters

authors： Fong JT,Nimlamool W,Falk MM

更新日期：2014-03-03 00:00:00

abstract：:Charybdotoxin (ChTX), a 4.3 kDa polypeptide toxin from the venom of the scorpion Leiurus quinquestriatus, blocks both a Ca-activated K channel (IC50 approximately 15 nM) and a Ca-independent voltage-gated K channel (IC50 approximately 40 nM) in rat brain synaptosomes. These results indicate that in this preparation Ch...

journal_title：FEBS letters

authors： Schneider MJ,Rogowski RS,Krueger BK,Blaustein MP

更新日期：1989-07-03 00:00:00

abstract：:Present analysis procedures for NMR structure determination of macromolecules presuppose fixed internuclear distances. Improvement of the precision of the requisite NOE information has stimulated the use of more quantitative distance constraints thus necessitating examination as to whether the assumption of a rigid mo...

journal_title：FEBS letters

authors： LeMaster DM,Kay LE,Brünger AT,Prestegard JH

更新日期：1988-08-15 00:00:00

abstract：:Thymocytes previously loaded with quin 2 were rapidly permeabilized by the photon activation of erythrosin and the rate of permeabilization monitored by measuring fluorimetrically the increasing saturation of quin 2 with calcium. The extent of permeabilization was assessed also by the loss of [3H]quin 2 from the thymo...

journal_title：FEBS letters

authors： Yonuschot G,Matthews EK,Corps AN,Metcalfe JC

更新日期：1987-03-23 00:00:00

abstract：:Proline-rich tyrosine kinase2 (Pyk2) is a cytoplasmic tyrosine kinase related to focal adhesion kinase. Pyk2 expression has been known to be restricted to neuronal and hematopoietic cells and Pyk2 tyrosine phosphorylation and its kinase activity is important for the function of monocytes/macrophages. In NB4 acute prom...

journal_title：FEBS letters

authors： Park MH,Park SY,Kim Y

更新日期：2008-02-06 00:00:00

abstract：:Chromatography of the soluble porcine lymphocyte phospholipase C on cellulose phosphate resolves the activity into two species. An HPLC method is described for separating the enzyme products, Ins 1,2 greater than P and Ins 1P. Use of these methods reveals that the two iso-enzymes liberate, with a high degree of reprod...

journal_title：FEBS letters

authors： Carter HR,Bird IM,Smith AD

更新日期：1986-08-11 00:00:00

abstract：:The effects of mildly acidic conditions on the free energy of unfolding (DeltaG(u)(buff)) of the pore-forming alpha-hemolysin (alphaHL) from Staphylococcus aureus were assessed between pH 5.0 and 7.5 by measuring intrinsic tryptophan fluorescence, circular dichroism and elution time in size exclusion chromatography du...

journal_title：FEBS letters

authors： Bortoleto RK,Ward RJ

更新日期：1999-10-15 00:00:00