Abstract:
:Osteoblasts and adipocytes are believed to share a common progenitor. Peroxisome proliferator-activated receptor gamma (PPARgamma) plays a key role in the switching of these two cell lineages. Here, we demonstrated the differentiation of ES cells into an osteoblastic lineage using siRNA against PPARgamma without the addition of any osteogenic factors. We found that PPARgamma-siRNA downregulated the expression of aP2 mRNA and lipid accumulation, whereas it upregulated the expression of osteocalcin and calcium deposition. These results suggested that ES cells were directed into an osteoblastic lineage. Therefore, transient suppression using PPARgamma-siRNA may be a novel tool to induce differentiation of ES cells into osteoblasts.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Yamashita A,Takada T,Nemoto K,Yamamoto G,Torii Rdoi
10.1016/j.febslet.2006.06.057subject
Has Abstractpub_date
2006-07-24 00:00:00pages
4121-5issue
17eissn
0014-5793issn
1873-3468pii
S0014-5793(06)00774-5journal_volume
580pub_type
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