Abstract:
:A tropomyosin-binding protein (app. Mr 17000) was detected in equine platelet preparations by a gel overlay technique. Its isolation, amino acid and partial sequence analyses have shown it to be histone H2B. As with a similar protein from pig platelet preparations [der Terrossian et al. (1983) FEBS Lett. 152, 202-206], it inhibits Mg2+-dependent actomyosin S1 ATPase. This inhibition is partially reversed in the presence of calmodulin and Ca2+ but is not potentiated, unlike troponin-I, by tropomyosin. This protein, along with the other histones, is almost certainly derived from a low level of contaminating nucleated cells in most platelet preparations.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Stewart DI,Golosinska K,Smillie LBdoi
10.1016/0014-5793(83)81130-2subject
Has Abstractpub_date
1983-06-27 00:00:00pages
129-32issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(83)81130-2journal_volume
157pub_type
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