Abstract:
:Insolubility in non-ionic detergents such as Triton X-100 is a widely used biochemical criterion for characterization of membrane domains. We report here a novel green fluorescent protein fluorescence-based approach to directly determine detergent insolubility of specific membrane proteins. We have applied this method to explore the detergent resistance of an important G-protein coupled receptor, the serotonin1A (5-HT1A) receptor. Our results show, for the first time, that a small yet significant fraction of the 5-HT1A receptor exhibits detergent insolubility. These results are validated by control experiments involving fluorescent lipid probes and protein markers. Our results assume relevance in the context of localization of the 5-HT1A receptor in membrane domains and its significance in receptor function and signaling.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kalipatnapu S,Chattopadhyay Adoi
10.1016/j.febslet.2004.09.055keywords:
subject
Has Abstractpub_date
2004-10-22 00:00:00pages
455-60issue
3eissn
0014-5793issn
1873-3468pii
S0014579304011846journal_volume
576pub_type
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