Crystal structure and molecular mechanism of an aspartate/glutamate racemase from Escherichia coli O157.

abstract：:Analyzing the primary structure we predicted that calponin may interact with phospholipids. In order to check this suggestion we investigated the interaction of calponin with phospholipids by ultracentrifugation, light scattering, vesicles leakage and differential scanning calorimetry. In agreement with our prediction...

journal_title：FEBS letters

authors： Bogatcheva NV,Gusev NB

更新日期：1995-09-04 00:00:00

abstract：:The sialic acid of complex N-glycans can be biochemically engineered by substituting the physiological precursor N-acetylmannosamine with non-natural N-acylmannosamines. The Kv3.1 glycoprotein, a neuronal voltage-gated potassium channel, contains sialic acid. Western blots of the Kv3.1 glycoprotein isolated from trans...

journal_title：FEBS letters

authors： Hall MK,Reutter W,Lindhorst T,Schwalbe RA

更新日期：2011-10-20 00:00:00

abstract：:The labile iron pool of cells (LIP) constitutes the primary source of metabolic and catalytically reactive iron in the cytosol. We studied LIP homeostasis in K562 cells using the fluorescent metal-sensitive probe calcein. Following brief exposure to iron(II) salts or to oxidative or reductive stress, LIP rose by up to...

journal_title：FEBS letters

authors： Breuer W,Epsztejn S,Cabantchik ZI

更新日期：1996-03-18 00:00:00

abstract：:A synthetic form of human pancreatic growth hormone releasing factor (GRF-44-NH2) was shown to be a potent stimulator of growth hormone (GH) secretion and cellular cyclic AMP levels in cultured sheep pituitary cells. A small dose-dependent stimulation of prolactin secretion was also observed. Somatostatin (0.5 microM)...

journal_title：FEBS letters

authors： Law GJ,Ray KP,Wallis M

更新日期：1984-01-23 00:00:00

abstract：:Gene array profiling of RNA from cells engineered to express a dominant-negative version of the ETS family member transcription factor Pea3 (polyomavirus enhancer activator 3) identified WT1 as a candidate downstream gene. Given the co-expression of WT1 and Pea3 in developing kidney and breast tissue undergoing mesenc...

journal_title：FEBS letters

authors： Discenza MT,Vaz D,Hassell JA,Pelletier J

更新日期：2004-02-27 00:00:00

abstract：:In order to study the function of the bovine MAP4 microtubule-binding domain (the assembly-promoting (AP) sequence region), a fragment corresponding to the AP sequence region was prepared using an Escherichia coli expression system. When the fragment was mixed with purified tubulin at 37 degrees C, the fragment caused...

journal_title：FEBS letters

authors： Katsuki M,Tokuraku K,Nakagawa H,Murofushi H,Kotani S

更新日期：1997-11-24 00:00:00

abstract：:The 27 kDa platelet membrane protein (Gn27) that binds [alpha-32P]GTP on nitrocellulose blots of SDS-polyacrylamide gels [(1987) Biochem. J. 245, 617-620] was compared with other low molecular mass GTP-binding proteins. Platelet membranes also contained 21 kDa proteins that bound anti-ras p21 antibody and 22-23 kDa pr...

journal_title：FEBS letters

authors： Bhullar RP,Haslam RJ

更新日期：1988-09-12 00:00:00

abstract：:Farnesoid X receptor (FXR) is highly expressed in liver and intestine where it controls bile acid (BA), lipid and glucose homeostasis. Here we show that FXR is expressed and functional, as assessed by target gene expression analysis, in human islets and beta-cell lines. FXR is predominantly cytosolic-localized in the ...

journal_title：FEBS letters

authors： Popescu IR,Helleboid-Chapman A,Lucas A,Vandewalle B,Dumont J,Bouchaert E,Derudas B,Kerr-Conte J,Caron S,Pattou F,Staels B

更新日期：2010-07-02 00:00:00

abstract：:The early response to bacterial inoculation has been investigated and two Arabidopsis genes, ap3.3a and ap4.3a have been characterized. The AP3.3A protein showed high identity to centrin, a ubiquitous cytoskeletal protein first identified in unicellular green alga. Amino-acid sequence analyses of the AP4.3A protein in...

journal_title：FEBS letters

authors： Cordeiro MC,Piqueras R,de Oliveira DE,Castresana C

更新日期：1998-09-04 00:00:00

abstract：:The major anthocyanin in A. thaliana is a cyanidin derivative modified by glycosylation as well as by the addition of three acyl moieties: malonyl, p-coumaroyl, and sinapoyl. We have isolated a member of the BAHD acyltransferase family which catalyzes this malonylation reaction by combining a reverse genetics approach...

journal_title：FEBS letters

authors： D'Auria JC,Reichelt M,Luck K,Svatos A,Gershenzon J

更新日期：2007-03-06 00:00:00

abstract：:Cellular homeostasis is linked tightly to mitochondrial functions. Some damage to mitochondrial proteins and nucleic acids can lead to the depolarization of the inner mitochondrial membrane, thereby sensitizing impaired mitochondria for selective elimination by autophagy. Mitochondrial dysfunction is one of the key as...

journal_title：FEBS letters

authors： Tanaka A

更新日期：2010-04-02 00:00:00

abstract：:Contactin and TAG-1 are glycan phosphatidyl inositol (GPI)-anchored cell adhesion molecules that play a crucial role in the organization of axonal subdomains at the node of Ranvier of myelinating fibers. Contactin and TAG-1 mediate axo-glial selective interactions in association with Caspr-family molecules at paranode...

journal_title：FEBS letters

authors： Labasque M,Faivre-Sarrailh C

更新日期：2010-05-03 00:00:00

abstract：:Free and membrane-bound polyribosomes were isolated from the livers of untreated and clofibrate-treated male C57B1/6 mice. The in vitro translation products were investigated in a rabbit reticulocyte cell-free system by immunoprecipitation of cytosolic epoxide hydrolase, catalase and albumin. The soluble forms of epox...

journal_title：FEBS letters

authors： Joste V,Meijer J

更新日期：1989-05-22 00:00:00

abstract：:Unstimulated human fibrosarcoma cells (HT1080) constitutively secrete matrix metalloproteinase 2 (MMP 2) as a proenzyme requiring proteolytic cleavage by membrane type-1 MMP (MT1 MMP) for activation. Physiological and pharmacological stimuli induce clustering of MT1 MMP/tissue inhibitor of MMP 2 "receptors", promoting...

journal_title：FEBS letters

authors： Atkinson SJ,English JL,Holway N,Murphy G

更新日期：2004-05-21 00:00:00

abstract：:Oogenesis of amphibians is an atypical situation in which histone mRNA is polyadenylated. The poly(A) tract on H4 mRNA has been examined by S1 nuclease analysis. Throughout oogenesis the poly(A) tract is very short, and nonexistent on some mRNA molecules. The poly(A) tract is completely removed during maturation of th...

journal_title：FEBS letters

authors： Ballantine JE,Woodland HR

更新日期：1985-01-28 00:00:00

abstract：:In this review, we summarize the currently available information on the membrane topology of some key members of the human ABC protein subfamilies, and present the predicted domain arrangements. In the lack of high-resolution structures for eukaryotic ABC transporters this topology is based only on prediction algorith...

journal_title：FEBS letters

authors： Tusnády GE,Sarkadi B,Simon I,Váradi A

更新日期：2006-02-13 00:00:00

abstract：:Auxin increases phospholipase A(2) activity within 2min (Paul, R., Holk, A. and Scherer, G.F.E. (1998) Fatty acids and lysophospholipids as potential second messengers in auxin action. Rapid activation of phospholipase A(2) activity by auxin in suspension-cultured parsley and soybean cells. Plant J. 16, 601-611) and t...

journal_title：FEBS letters

authors： Scherer GF,Zahn M,Callis J,Jones AM

更新日期：2007-09-04 00:00:00

abstract：:Small RNAs (miR159-like RNAs) identical to some plant miR159s were found in Xenopus tropicalis miRNA cDNA libraries (30 miRNA families consisting of 75 unique sequences). Preferential expression of this RNA species was found in neural tissues during development. A sequence matching to this RNA species was not found in...

journal_title：FEBS letters

authors： Hikosaka A,Takaya K,Jinno M,Kawahara A

更新日期：2007-06-26 00:00:00

abstract：:Sialic acids comprise a family of terminal sugars essential for a variety of biological recognition systems. UDP-N-acetylglucosamine 2-epimerase catalyzes the first step of their biosynthesis. Periodate-oxidized UDP-N-acetylglucosamine, namely 2',3'-dialdehydo-UDP-alpha-D-N-acetylglucosamine, was found to be an effect...

journal_title：FEBS letters

authors： Blume A,Chen H,Reutter W,Schmidt RR,Hinderlich S

更新日期：2002-06-19 00:00:00

abstract：:The roles of mild uncoupling caused by free fatty acids (mediated by plant uncoupling mitochondrial protein (PUMP) and ATP/ADP carrier (AAC)) and non-coupled respiration (alternative oxidase (AO)) on H(2)O(2) formation by plant mitochondria were examined. Both laurate and oleate prevent H(2)O(2) formation dependent on...

journal_title：FEBS letters

authors： Casolo V,Braidot E,Chiandussi E,Macrì F,Vianello A

更新日期：2000-05-26 00:00:00

abstract：:The C-terminal regions of Kv1 K+ channels show little conservation between isoforms except for the last four C-terminal residues, (E/L)TDV, which are well conserved from Drosophila to man. Deletions of the 4, 16, and 57 C-terminal residues of the human Kv1.5 channel did not affect whole cell current amplitude, midpoin...

journal_title：FEBS letters

authors： Uebele VN,Yeola SW,Snyders DJ,Tamkun MM

更新日期：1994-02-28 00:00:00

abstract：:A protein factor which inhibited adenylate cyclase was purified to apparent homogeneity from rat brain and identified as the beta gamma-subunits of the GTP-binding regulatory proteins of adenylate cyclase. (i) The beta gamma-subunits (protein factor) inhibited the partially purified catalytic unit of adenylate cyclase...

journal_title：FEBS letters

authors： Enomoto K,Asakawa T

更新日期：1986-06-23 00:00:00

abstract：:Enzymes from extremely halophilic archaea are readily denatured in the absence of a high salt concentration. However, we have observed here that a nucleoside diphosphate kinase prepared from Halobacterium salinarum was active and stable in the absence of salt, though it has the amino acid composition characteristic of...

journal_title：FEBS letters

authors： Ishibashi M,Tokunaga H,Hiratsuka K,Yonezawa Y,Tsurumaru H,Arakawa T,Tokunaga M

更新日期：2001-03-30 00:00:00

abstract：:WT1 was isolated as a tumor suppressor gene of Wilms tumor. However, high expression of WT1 correlates with poor prognosis in acute leukemia. In addition suppression of WT1 expression by WT1 anti-sense oligonucleotide inhibits proliferation of leukemia cells, suggesting that WT1 is important for their proliferation. T...

journal_title：FEBS letters

authors： Murata Y,Kudoh T,Sugiyama H,Toyoshima K,Akiyama T

更新日期：1997-06-02 00:00:00

abstract：:A novel GTP binding protein (G protein) alpha subunit cDNA was isolated from a T cell leukemia cell line, Jurkat, utilizing polymerase chain reaction (PCR). The predicted amino acid sequence of this G protein alpha subunit showed the highest identity (96.6%) to bovine cone cell-specific transducin (Tc alpha). The orga...

journal_title：FEBS letters

authors： Kubo M,Hirano T,Kakinuma M

更新日期：1991-10-21 00:00:00

abstract：:Using antibody against the Rho binding domain of ROKalpha, two neuronal phosphoproteins of 62 and 80 kDa were co-immunoprecipitated from brain extracts. Peptide analysis revealed their identity as collapsin response mediator proteins (CRMPs); p62 was CRMP-2 whereas p80 was a novel splice form of CRMP-1 with an extende...

journal_title：FEBS letters

authors： Leung T,Ng Y,Cheong A,Ng CH,Tan I,Hall C,Lim L

更新日期：2002-12-18 00:00:00

abstract：:Glycosaminoglycans are known to be associated with extracellular amyloid deposits of various amyloidogenic proteins. In this study, we found that the glycosaminoglycan heparin greatly accelerates the elongation step in fibril formation by the N-terminal 1-83 fragment of human apolipoprotein A-I (apoA-I), especially in...

journal_title：FEBS letters

authors： Mikawa S,Mizuguchi C,Nishitsuji K,Baba T,Shigenaga A,Shimanouchi T,Sakashita N,Otaka A,Akaji K,Saito H

更新日期：2016-10-01 00:00:00

abstract：:RT-PCR was undertaken on total RNA extracts from bone and scales of the rainbow trout, Oncorhynchus mykiss. The rainbow trout estrogen receptor (ER)-specific primers used amplified a single product of expected size from each tissue which, using Southern blotting, strongly hybridized with a 32P-labelled rtER probe unde...

journal_title：FEBS letters

authors： Armour KJ,Lehane DB,Pakdel F,Valotaire Y,Graham R,Russell RG,Henderson IW

更新日期：1997-07-07 00:00:00

abstract：:The pH dependence of spin-spin coupling constants J (P-O-P) of ADP and ATP has been determined from the phosphorus NMR spectra at 0 degrees. The variation curves have been interpreted as titration curves. These give different pK values. Comparison with the tripolyphosphate shows the existence of triphosphate chain str...

journal_title：FEBS letters

authors： Ellenberger M,Brehamet L,Villemin M,Toma F

更新日期：1970-06-01 00:00:00

abstract：:Epigallocatechin-3-gallate (EGCG) is a major component of green tea polyphenols which displays potential properties of anticancer and neuroprotection. Here we show that protection of motor neuron by EGCG is associated with regulating glutamate level in organotypic culture of rat spinal cord. In this model, EGCG blocke...

journal_title：FEBS letters

authors： Yu J,Jia Y,Guo Y,Chang G,Duan W,Sun M,Li B,Li C

更新日期：2010-07-02 00:00:00