Purification and functional reconstitution of a truncated human Na(+)/glucose cotransporter (SGLT1) expressed in E. coli.

abstract：:Over the past several years, the study of a hereditary tumor syndrome, tuberous sclerosis complex (TSC), has shed light on the regulation of cellular proliferation and growth. TSC is an autosomal dominant disorder that is due to inactivating mutations in TSC1 or TSC2 and characterized by benign tumors (hamartomas) inv...

journal_title：FEBS letters

authors： Han JM,Sahin M

更新日期：2011-04-06 00:00:00

abstract：:We have attempted to elucidate the mechanism of apoptotic cell death induced by hypoxia (very low oxygen conditions) in neuronal cells. Human neuroblastoma SK-N-MC cells under hypoxic conditions resulted in apoptosis in a time-dependent manner estimated by DNA fragmentation assay and nuclear morphology stained with fl...

journal_title：FEBS letters

authors： Araya R,Uehara T,Nomura Y

更新日期：1998-11-13 00:00:00

abstract：:We propose a unified framework for the identification of functional interaction structures of biomolecular networks in a way that leads to a new experimental design procedure. In developing our approach, we have built upon previous work. Thus we begin by pointing out some of the restrictions associated with existing s...

journal_title：FEBS letters

authors： Cho KH,Choo SM,Wellstead P,Wolkenhauer O

更新日期：2005-08-15 00:00:00

abstract：:Matrix metalloproteinase-13 (MMP-13) plays a critical role in parathyroid hormone (PTH)-induced bone resorption. PTH acts via protein kinase A (PKA) to phosphorylate and stimulate the transactivation of Runx2 for MMP-13 promoter activation. We show here that PTH stimulated Runx2 phosphorylation in rat osteoblastic cel...

journal_title：FEBS letters

authors： Selvamurugan N,Shimizu E,Lee M,Liu T,Li H,Partridge NC

更新日期：2009-04-02 00:00:00

abstract：:Eucaryotic DNA is punctuated by many A+T-rich segments that we named A+T-rich linkers. Two types of these A+T-rich linkers can be distinguished: (i) isolated A+T-rich linkers, and (ii) A+T-rich linkers crowded in clusters. We have analysed the distribution of A+T-rich linker across the alpha- and beta-globin gene doma...

journal_title：FEBS letters

authors： Moreau J,Scherrer K

更新日期：1987-08-31 00:00:00

abstract：:Incubation of rat adipocytes with the phorbol ester TPA caused a dose-dependent, persistent increase in phosphatidate phosphohydrolase activity and decrease in fatty acyl-CoA synthetase activity. Half-maximal effects were obtained with 1-2 X 10(-10) M TPA. TPA did not alter basal or noradrenaline-stimulated lipolysis....

journal_title：FEBS letters

authors： Hall M,Taylor SJ,Saggerson ED

更新日期：1985-01-07 00:00:00

abstract：:In the ameboid eukaryote Dictyostelium discoideum, chemotactic stimulation by cAMP induces an increase of intracellular cGMP and subsequently the phosphorylation of myosin heavy chain II. Resistance to high osmotic stress also requires transient increases of intracellular cGMP and phosphorylation of myosin heavy chain...

journal_title：FEBS letters

authors： Kuwayama H,Van Haastert PJ

更新日期：1998-03-13 00:00:00

abstract：:Treatment of U937 cells with dolichyl phosphate led to an increase in the activity of the ICE family protease CPP32, accompanied with cleavage of pre-CPP32 to generate p17. Peptide inhibitors YVAD-cmk and Z-Asp-CH2-DCB (specific to ICE) and DEVD-CHO (specific to CPP32) blocked the dolichyl phosphate-induced apoptosis....

journal_title：FEBS letters

authors： Yokoyama Y,Okubo T,Ozawa S,Nagai F,Ushiyama K,Kano I,Shioda M,Kubo H,Takemura M,Namiki H,Yasugi E,Oshima M,Seyama Y,Kano K

更新日期：1997-07-21 00:00:00

abstract：:Folding of nascent chains resembles the decoding of spoken language in that information is emitted as a unidirectional, one-dimensional string of elements, with higher structures and long-distance interactions emerging with time. Applying a "pseudolinguistic' analysis of structure to a set of all 36 possible six-stran...

journal_title：FEBS letters

authors： Gross M

更新日期：1996-07-29 00:00:00

abstract：:The intestinal epithelium is equipped with sensing receptor mechanisms that interact with luminal microorganisms and nutrients to regulate barrier function and gut immune responses, thereby maintaining intestinal homeostasis. Herein, we clarify the role of the extracellular calcium-sensing receptor (CaSR) using intest...

journal_title：FEBS letters

authors： Cheng SX,Lightfoot YL,Yang T,Zadeh M,Tang L,Sahay B,Wang GP,Owen JL,Mohamadzadeh M

更新日期：2014-11-17 00:00:00

abstract：:The structure determination of apotransketolase and the comparison of its three-dimensional structure with that of the holoenzyme has revealed that no large conformational changes are associated with cofactor binding. Two loops at the active site are flexible in the apoenzyme which enables ThDP to reach its binding si...

journal_title：FEBS letters

authors： Sundström M,Lindqvist Y,Schneider G

更新日期：1992-11-30 00:00:00

abstract：:Vascular endothelial cells and neutrophils synthesize and release potent vasodilatatory factors, i.e. endothelium-derived relaxing factors (EDRF) and neutrophil-derived relaxing factors (NDRF). One EDRF has been identified as nitric oxide (NO) derived from arginine. We studied the synthesis and release of NO from huma...

journal_title：FEBS letters

authors： Schmidt HH,Seifert R,Böhme E

更新日期：1989-02-27 00:00:00

abstract：:Amino acid sequences coded for by 13 different genes of mammalian mitochondrial DNA (mtDNA) including 8 unassigned open reading frames (URFs) were compared in pairs. It was found that significant homologies exist among the amino acid sequences of the three URFs (URF2, URF4 and URF5) with a probability of occurrence of...

journal_title：FEBS letters

authors： Kikuno R,Miyata T

更新日期：1985-09-09 00:00:00

abstract：:Autophagy is a bulk degradation process conserved among eukaryotes. In macro-autophagy, autophagosomes sequester cytoplasmic components and deliver their contents to lysosomes/vacuoles. Autophagosome formation requires the conjugation of Atg8, a ubiquitin-like protein, to phosphatidylethanolamine (PE). Here we report ...

journal_title：FEBS letters

authors： Hanada T,Satomi Y,Takao T,Ohsumi Y

更新日期：2009-04-02 00:00:00

abstract：:The malaria parasite has for long been thought to escape host complement attack as a survival strategy. However, it was only recently that complement evasion mechanisms of the parasite were described. Simultaneously, the role of complement in antibody-mediated naturally acquired and vaccine-induced protection against ...

journal_title：FEBS letters

authors： Kiyuka PK,Meri S,Khattab A

更新日期：2020-08-01 00:00:00

abstract：:Read-through or mutations of a stop codon resulting in translation of the 3'-UTR produce potentially toxic C-terminally extended proteins. However, quality control mechanisms for such proteins are poorly understood in mammalian cells. Here, a comprehensive analysis of the 3'-UTRs of genes associated with hereditary di...

journal_title：FEBS letters

authors： Hashimoto S,Nobuta R,Izawa T,Inada T

更新日期：2019-04-01 00:00:00

abstract：:In this study we used the fungal antibiotic brefeldin A (BFA) to analyze its effect on viral replication. Analysis by electron microscopy demonstrated that no viral particles were observed in cells treated before the onset of viral replication. In the presence of BFA expression of IE2, MCP, pUL104, pUL56 and pUL89 wer...

journal_title：FEBS letters

authors： Kregler O,Schilf R,Lander A,Bannert N,Bogner E

更新日期：2009-04-02 00:00:00

abstract：:Analyzing the primary structure we predicted that calponin may interact with phospholipids. In order to check this suggestion we investigated the interaction of calponin with phospholipids by ultracentrifugation, light scattering, vesicles leakage and differential scanning calorimetry. In agreement with our prediction...

journal_title：FEBS letters

authors： Bogatcheva NV,Gusev NB

更新日期：1995-09-04 00:00:00

abstract：:Protein kinase C (PKC) may be involved in growth regulation. In the present study the relationship between body weight, and thereby age, and the activity of PKC in muscle as well as in rapidly growing overloaded muscle were investigated. PKC activity in music was linearly inversely correlated to rat weight in both sol...

journal_title：FEBS letters

authors： Richter EA,Nielsen NB

更新日期：1991-09-02 00:00:00

abstract：:Ghrelin is a novel gastrointestinal peptide that stimulates growth hormone secretion, food intake, and body weight gain. Increased ghrelin secretion has been reported in such negative energy states as starvation and low body weight. We investigated the dynamics of ghrelin in rats with streptozotocin-induced diabetes, ...

journal_title：FEBS letters

authors： Masaoka T,Suzuki H,Hosoda H,Ota T,Minegishi Y,Nagata H,Kangawa K,Ishii H

更新日期：2003-04-24 00:00:00

abstract：:Incubation of human thrombocytes in the presence of [3H]palmitic acid leads to incorporation of this fatty acid into the alpha subunit of Gs as described [Linder et al., Proc. Natl. Acad. Sci. USA 90 (1993) 3675-3679; Degtyarev et al., Biochemistry 32 (1993) 8057-8061] but also into the catalyst of adenylyl cyclase wh...

journal_title：FEBS letters

authors： Mollner S,Beck K,Pfeuffer T

更新日期：1995-09-11 00:00:00

abstract：:Protein kinase B (PKB, also named as Akt or RAC-protein kinase), that is activated by cellular stress such as heat shock and hyperosmotic treatment, was revealed to be activated by oxidative stress and by chemical stressors of CdCl2 and NaAsO2 by measuring the activity of the enzyme immunoprecipitated from the transfe...

journal_title：FEBS letters

authors： Konishi H,Matsuzaki H,Tanaka M,Takemura Y,Kuroda S,Ono Y,Kikkawa U

更新日期：1997-06-30 00:00:00

abstract：:A novel rapid polymerase chain reaction (PCR)-based technique for N-terminal attachment of green fluorescent protein (GFP) to a yeast protein is described. Genomic integration of a PCR-generated loxPkanMX4loxP-yEGFP fusion cassette immediately upstream of the open reading frame (ORF) allows for selection of G418 resis...

journal_title：FEBS letters

authors： Prein B,Natter K,Kohlwein SD

更新日期：2000-11-17 00:00:00

abstract：:In kidney cortex tubules isolated from fed rabbits L-alanine is not utilized as glucose precursor, when added as a sole substrate. However, this amino acid decreases gluconeogenesis from low (up to 1 mM) 2-oxoglutarate concentrations and stimulates this process at higher (2.5-10 mM) ketoacid contents in the suspension...

journal_title：FEBS letters

authors： Zabłocki K,Bryła J

更新日期：1989-12-18 00:00:00

abstract：:Increased expression of low voltage-activated, T-type Ca(2+) channels has been correlated with a variety of cellular events including cell proliferation and cell cycle kinetics. The recent cloning of three genes encoding T-type alpha(1) subunits, alpha(1G), alpha(1H) and alpha(1I), now allows direct assessment of thei...

journal_title：FEBS letters

authors： Chemin J,Monteil A,Briquaire C,Richard S,Perez-Reyes E,Nargeot J,Lory P

更新日期：2000-07-28 00:00:00

abstract：:The two subunits of gamma-glutamyl transpeptidase (EC 2.3.2.2) are derived from a single-chain glycosylated precursor. A small fraction of the propeptide survives proteolytic processing in the rat kidney and has been purified by an immunoaffinity technique. The propeptide contains determinants for both the subunits an...

journal_title：FEBS letters

authors： Tate SS

更新日期：1986-01-01 00:00:00

abstract：:Heat shock (44 degrees C for 10 min) or ATP depletion by an uncoupler (CCCP for 20 min) is shown to result in stimulation of hsp68/70 synthesis in Ehrlich tumor cells. After 3 h of recovery, the cells become thermotolerant. Surprisingly, repeated ATP depletion caused by CCCP or rotenone (a respiratory inhibitor) treat...

journal_title：FEBS letters

authors： Gabai VL,Kabakov AE

更新日期：1993-08-02 00:00:00

abstract：:RNA-binding protein kinase from amphibian oocytes modifies serine and threonine residues in the molecules of substrates and utilizes both ATP and GTP. Low concentrations of heparin inhibit protein kinase. The foregoing suggests that this enzyme is casein kinase II. It is shown that RNA-binding proteins lack active for...

journal_title：FEBS letters

authors： Kandror KV,Stepanov AS

更新日期：1984-05-07 00:00:00

abstract：:Plasmid pGC20 containing the (dGC)9 insert in SmaI recognition site has been used to study the inhibition of cleavage by different restriction endonuclease due to Z-DNA formation in (dCG)10 sequence of the negatively supercoiled plasmid. Data obtained indicate the different sensitivity of restriction endonucleases to ...

journal_title：FEBS letters

authors： Lesnik EA,Bes'chetnikova ZhA,Maslova RN,Varshavsky JaM

更新日期：1991-03-11 00:00:00

abstract：:Acceptor stem variants of tRNA(fMet) (Escherichia coli) have been characterized by nuclear magnetic resonance. The wild type contains a C1-A72 mismatch pair which is crucial for its biological function. For comparison, the mismatch was replaced by regular pairs U1-A72 and C1-G72. Further variants contain an altered di...

journal_title：FEBS letters

authors： Zuleeg T,Vogtherr M,Schübel H,Limmer S

更新日期：2000-04-28 00:00:00