Acidic phospholipids directly inhibit DNA binding of mammalian DNA topoisomerase I.

abstract：:The relationship among the real-time trafficking of lung metastatic B16BL6 cells, metastatic potential, and the injected number of the cells was examined, since the smaller the number of tumor cells injected, the more clearly the immune defense may be observed. When 1x10(6) or 1x10(5) B16BL6 cells were injected into m...

journal_title：FEBS letters

authors： Kikkawa H,Imafuku H,Tsukada H,Oku N

更新日期：2000-02-11 00:00:00

abstract：:Tom1 (Target of Myb1) is suggested to be involved in the transport of ubiquitinated proteins, through the interaction of its GAT (GGA and Tom1) domain with ubiquitin. Here, we demonstrate that the three-helix bundle of Tom1-GAT has two ubiquitin-binding sites recognizing the hydrophobic Ile44 surface of ubiquitin. The...

journal_title：FEBS letters

authors： Akutsu M,Kawasaki M,Katoh Y,Shiba T,Yamaguchi Y,Kato R,Kato K,Nakayama K,Wakatsuki S

更新日期：2005-10-10 00:00:00

abstract：:The biological activity and the fate of SV40 DNA (minichromosomes, DNA I, DNA II, DNA III) were tested in culture cells by immunofluorescence staining and blot analysis. Following microinjection of 2-4 circular SV40 molecules (minichromosomes, DNA I, DNA II) into the cytoplasm or the nuclei of monkey and rat cells, T-...

journal_title：FEBS letters

authors： Graessmann A,Bumke-Vogt C,Buschhausen G,Bauer M,Graessmann M

更新日期：1985-01-01 00:00:00

abstract：:The OxyR protein is a transcriptional activator for a subset of peroxide stress-inducible genes, most of which are involved in defense systems against oxidative stress. Recently, it was demonstrated that purified OxyR has one intramolecular disulfide bond, which led to the proposal that the reversible disulfide bond f...

journal_title：FEBS letters

authors： Tao K

更新日期：1999-08-20 00:00:00

abstract：:Tumor suppressor WW domain-containing oxidoreductase (WWOX) is depleted in various cancer types. Here we report that WWOX is modified by small ubiquitin-like modifier (SUMO) proteins and represses DU145 prostate cancer tumorigenesis in a SUMOylation-dependent manner. Ectopic WWOX was shown to associate with SUMO2/3 or...

journal_title：FEBS letters

authors： Choi HJ,Park JH,Park JH,Lee KB,Oh SM

更新日期：2015-12-21 00:00:00

abstract：:Two alpha-type CK2-activated PKAs (CK2-aPKAIalpha and CK2-aPKAIIalpha) were biochemically characterized in vitro using GST-HBV core fusion protein (GST-Hcore) and GST-Hcore157B as phosphate acceptors. It was found that (i), in the absence of cAMP, these two CK2-aPKAs phosphorylated both Ser-170 and Ser-178 on GST-Hcor...

journal_title：FEBS letters

authors： Enomoto M,Sawano Y,Kosuge S,Yamano Y,Kuroki K,Ohtsuki K

更新日期：2006-02-06 00:00:00

abstract：:Heparin was found to stimulate the phosphorylation of histone H1 but not protamine sulfate catalyzed by Ca2+/phospholipid-dependent protein kinase (protein kinase C or PKC). The effect of heparin on histone H1 phosphorylation appeared to be due to an increase in phosphatidylserine affinity for PKC activation in the pr...

journal_title：FEBS letters

authors： Goueli SA,Hanten JA,Ahmed K

更新日期：1991-05-06 00:00:00

abstract：:As a SNARE binding protein, tomosyn has been reported to negatively regulate synaptic exocytosis via arresting syntaxin-1 and SNAP-25 into a nonfusogenic product that precludes synaptobrevin-2 entry, raising the question how the assembly of the SNARE complex is achieved. Here, we have investigated new functions of tom...

journal_title：FEBS letters

authors： Li Y,Wang S,Li T,Zhu L,Ma C

更新日期：2018-04-01 00:00:00

abstract：:Brain-type creatine kinase B-CK (EC 2.7.3.2) was purified from several chicken tissues, e.g. cardiac muscle, brain, gizzard and retina. Two major monomeric chicken B-CK subunits, designated Bb (basic) and Ba (acidic), which differ in isoelectric point, were separated by chromatofocusing in the presence of 8 M urea on ...

journal_title：FEBS letters

authors： Quest AF,Eppenberger HM,Wallimann T

更新日期：1990-03-26 00:00:00

abstract：:The 5.8S and 28S rRNA sequences of the oomycete Phytophthora megasperma were determined in order to study the secondary structure of these molecules and to assess the phylogenetic position of the oomycetes among the eukaryotes. Preliminary results point to an affiliation between the oomycetes, dinoflagellates and cili...

journal_title：FEBS letters

authors： Van der Auwera G,Chapelle S,De Wachter R

更新日期：1994-01-31 00:00:00

abstract：:The role of Na+/H+ exchange in protein kinase C-mediated effects in platelets was investigated by studying the effect of removal of extracellular Na+ ([Na+]e) on the different responses induced by phorbol 12-myristate 13-acetate (PMA) and 1,2-dioctanoylglycerol (diC8). None of the responses studied, namely, protein ph...

journal_title：FEBS letters

authors： Krishnamurthi S,Patel Y,Morgan WA,Wheeler-Jones CP,Kakkar VV

更新日期：1989-07-31 00:00:00

abstract：:Tissue-transglutaminase (t-TGase) is a family of calcium-dependent enzymes. A Ca2+-independent soluble enzyme, in addition to t-TGase, capable of incorporating polyamines into proteins was demonstrated in rat intestinal mucosa. The Ca2+-independent enzyme was stimulated 2- to 5-fold by Fe2+ and Co2+ ions but inhibited...

journal_title：FEBS letters

authors： Tsai YH,Lai WF,Wu YW,Johnson LR

更新日期：1998-09-18 00:00:00

abstract：:Incubation of rat adipocytes with the phorbol ester TPA caused a dose-dependent, persistent increase in phosphatidate phosphohydrolase activity and decrease in fatty acyl-CoA synthetase activity. Half-maximal effects were obtained with 1-2 X 10(-10) M TPA. TPA did not alter basal or noradrenaline-stimulated lipolysis....

journal_title：FEBS letters

authors： Hall M,Taylor SJ,Saggerson ED

更新日期：1985-01-07 00:00:00

abstract：:The induction of apoptosis by cell cycle regulator molecules under conditions optimal for exponential growth was examined in rat pheochromocytoma PC12 cells by overexpression of cyclins and cyclin-dependent kinases (cdks). By flow cytometry and by immunofluorescence, only cells overexpressing cdk4 or cyclin D1 underwe...

journal_title：FEBS letters

authors： Katayama K,Dobashi Y,Kitagawa M,Kamekura S,Kawai M,Kadoya Y,Kameya T

更新日期：2001-12-14 00:00:00

abstract：:In order to identify physiological activators of proteinase-activated receptor-2 (PAR-2), a peptide chloromethane inhibitor (biotinyl-Ser-Lys-Gly-Arg-CH2Cl) based on the cleavage site for activation of PAR-2 was synthesised and tested with 12 trypsin-like serine proteinases. The second-order rate constant (ki/Ki) for ...

journal_title：FEBS letters

authors： Fox MT,Harriott P,Walker B,Stone SR

更新日期：1997-11-17 00:00:00

abstract：:Stable liposomes were rendered pH-sensitive by complexation to a polymer that undergoes marked temperature- and pH-dependent water solubility changes. The N-isopropylacrylamide-methacrylic acid copolymer was prepared with or without octadecyl acrylate. At pH below the phase transition of the polymer, egg phosphatidylc...

journal_title：FEBS letters

authors： Meyer O,Papahadjopoulos D,Leroux JC

更新日期：1998-01-02 00:00:00

abstract：:Using PCR-based techniques, we have isolated and sequenced the full-length cDNAs that encode the human neuronal nAChR subunits alpha3,4,5,7 and beta2,3,4 in the neuroblastoma cell lines SH-SY5Y and/or IMR-32. The predicted nAChR beta3- and beta4-subunit proteins contain 458 and 498 amino acids, respectively. Except fo...

journal_title：FEBS letters

authors： Groot Kormelink PJ,Luyten WH

更新日期：1997-01-06 00:00:00

abstract：:Protein kinase A (PKA) signaling is essential for growth and virulence of the fungal pathogen Aspergillus fumigatus. Little is known concerning the regulation of this pathway in filamentous fungi. Employing liquid chromatography-tandem mass spectroscopy, we identified novel phosphorylation sites on the regulatory subu...

journal_title：FEBS letters

authors： Shwab EK,Juvvadi PR,Waitt G,Soderblom EJ,Moseley MA,Nicely NI,Steinbach WJ

更新日期：2017-11-01 00:00:00

abstract：:The protozoan parasite and human pathogen Entamoeba histolytica is protected against killing by its own lytic effector proteins. Amoebae withstand doses of amoebapores, their pore-forming polypeptides, that readily kill human Jurkat T cells. Moreover, the polypeptides do not bind to the amoebic surface membrane as evi...

journal_title：FEBS letters

authors： Andrä J,Berninghausen O,Leippe M

更新日期：2004-04-23 00:00:00

abstract：:gamma-Seminoprotein (gamma-Sm) is a human prostate-specific antigen and a serine protease judging from the complete amino acid sequence which shows extensive homology with the kallikrein family. The enzymatic activity of gamma-Sm was defined as a chymotrypsin-like activity using reduced and S-3-(trimethylated amino)pr...

journal_title：FEBS letters

authors： Akiyama K,Nakamura T,Iwanaga S,Hara M

更新日期：1987-12-10 00:00:00

abstract：:Analysis of 13 high-resolution protein X-ray crystal structures shows that 1204 (24%) of all the 4974 hydrogen bonds are of the bifurcated three-center type with the donor X-H opposing two acceptors A1, A2. They occur systematically in alpha-helices where 90% of the hydrogen bonds are of this type; the major component...

journal_title：FEBS letters

authors： Preissner R,Egner U,Saenger W

更新日期：1991-08-19 00:00:00

abstract：:The amino acid residue Asn141 of the restriction endonuclease EcoRI was proposed to make three hydrogen bonds to both adenine residues within the recognition sequence -GAATTC-. We have mutated Asn141 to alanine, aspartate, serine, and tyrosine. Only the serine mutant is active under normal buffer conditions although 1...

journal_title：FEBS letters

authors： Fritz A,Küster W,Alves J

更新日期：1998-10-30 00:00:00

abstract：:Maurotoxin (MTX) is a 34-residue toxin that has been isolated from the venom of the chactidae scorpion Scorpio maurus palmatus, and characterized. Together with Pi1 and HsTx1, MTX belongs to a family of short-chain four-disulfide-bridged scorpion toxins acting on potassium channels. However, contrary to other members ...

journal_title：FEBS letters

authors： Carlier E,Fajloun Z,Mansuelle P,Fathallah M,Mosbah A,Oughideni R,Sandoz G,Di Luccio E,Geib S,Regaya I,Brocard J,Rochat H,Darbon H,Devaux C,Sabatier JM,de Waard M

更新日期：2001-02-02 00:00:00

abstract：:The 42 kDa precursor of surfactant protein B generates the 79 residue mature SP-B polypeptide, which belongs to the family of saposin-like proteins and has unique functional roles in pulmonary surfactant. From sequence comparisons it has been suggested that proSP-B, in addition to SP-B, contains two saposin-like domai...

journal_title：FEBS letters

authors： Zaltash S,Johansson J

更新日期：1998-02-13 00:00:00

abstract：:Low density lipoproteins are highly sensitive to oxidation by copper salts, and such peroxidation is accompanied by macrophage scavenger receptor recognition. This study shows that fresh human atherosclerotic material (aneurysms and endarterectomies) can contain detectable amounts of redox active iron and copper that ...

journal_title：FEBS letters

authors： Swain J,Gutteridge JM

更新日期：1995-07-24 00:00:00

abstract：:Annexin-I is a calcium dependent phospholipid binding and phospholipase A2 (PLA2) inhibitory protein. A 'substrate depletion' model has been proposed for the mechanism of PLA2 inhibition by annexin-I in studies with 14 to 18 kDa PLA2s. Herein, we have studied the inhibition mechanism using 100 kDa cytosolic PLA2 from ...

journal_title：FEBS letters

authors： Kim KM,Kim DK,Park YM,Kim CK,Na DS

更新日期：1994-05-02 00:00:00

abstract：:Over 400 human proteases documented in secondary databases can already be delineated in genomic sequence. A Genome Ontology annotation of 30585 sequences in the provisional human proteome set recognises 498 proteases, i.e. 1.6%. Homology searches against finished sequence and comparisons between mouse and zebrafish ar...

journal_title：FEBS letters

authors： Southan C

更新日期：2001-06-08 00:00:00

abstract：:Myocyte stress 1 (MS1) is a recently described striated muscle actin-binding protein that is up-regulated in the early stages of pressure overload left ventricular hypertrophy. The aim of this study was to determine whether MS1 induces cellular hypertrophy and protects against apoptosis. Over-expressed MS1 co-localize...

journal_title：FEBS letters

authors： Koekemoer AL,Chong NW,Goodall AH,Samani NJ

更新日期：2009-09-03 00:00:00

abstract：:Numerous biological proteins exhibit intrinsic disorder at their termini, which are associated with multifarious functional roles. Here, we show the surprising result that an increased percentage of terminal short transiently disordered regions with enhanced flexibility (TstDREF) is associated with accelerated folding...

journal_title：FEBS letters

authors： Mallik S,Ray T,Kundu S

更新日期：2017-07-01 00:00:00

abstract：:APE-independent base excision repair (BER) pathway plays an important role in the regulation of DNA repair mechanisms. In this study it has been found that recently discovered tyrosyl-DNA phosphodiesterase 1 (Tdp1) catalyzes the AP site cleavage reaction to generate breaks with the 3'- and 5'-phosphate termini. The re...

journal_title：FEBS letters

authors： Lebedeva NA,Rechkunova NI,Lavrik OI

更新日期：2011-02-18 00:00:00