Abstract:
:gamma-Seminoprotein (gamma-Sm) is a human prostate-specific antigen and a serine protease judging from the complete amino acid sequence which shows extensive homology with the kallikrein family. The enzymatic activity of gamma-Sm was defined as a chymotrypsin-like activity using reduced and S-3-(trimethylated amino)propylated lysozyme and insulin-oxidized A and B chains as substrates. The -Leu/Ser- peptide bond of lysozyme was rapidly hydrolyzed by gamma-Sm. gamma-Sm also hydrolyzed the -Phe/Glu- of lysozyme and the -Leu/Cys(SO3H)- of insulin B chain. Insulin A chain and arginyl- or lysyl-linkage of these proteins were not hydrolyzed by gamma-Sm at all.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Akiyama K,Nakamura T,Iwanaga S,Hara Mdoi
10.1016/0014-5793(87)81151-1subject
Has Abstractpub_date
1987-12-10 00:00:00pages
168-72issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(87)81151-1journal_volume
225pub_type
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