Hirudin or hirudin-like factor - that is the question: insights from the analyses of natural and synthetic HLF variants.

Abstract:

:The hirudin-like factor 1 (HLF1) of Hirudo medicinalis belongs to a new class of leech-derived factors. In previous investigations, HLF1 did not exhibit anticoagulatory activities. Here, we describe the analysis of natural and synthetic variants of HLF1 and HLF-Hyb, a yet uncharacterized member of the HLF family. Modifications within the N terminus of HLF1 have a strong impact on its activity. Some variants of HLF1 exhibit thrombin-inhibiting activity comparable to hirudins, whereas others have reduced or no activity. The analyses of HLF-Hyb variants revealed a strong impact of the central globular domain on activity. Our results indicate a comparable mode of action of hirudins and thrombin-inhibiting HLF variants. Finally, we propose and discuss criteria for classifying hirudins and HLFs.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Müller C,Lukas P,Böhmert M,Hildebrandt JP

doi

10.1002/1873-3468.13683

subject

Has Abstract

pub_date

2020-03-01 00:00:00

pages

841-850

issue

5

eissn

0014-5793

issn

1873-3468

journal_volume

594

pub_type

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