Abstract:
:Histone acetyltransferase activity was measured in isolated nuclei during the synchronous cell cycle of the myxomycete Physarum polycephalum. Nuclei were incubated with [14C]acetyl-coenzyme A and an excess of exogenous calf thymus histones. The activity is periodic during the cell cycle; it rises during the S-phase to reach a maximum in the early G2-period with a decline in mid and late G2. Comparison of the pattern of enzyme activity with the in vivo acetylation of histones during the cell cycle reveals that the enzyme activity does not wholly determine the acetylation state, indicating that other factors, including possibly the structural state of chromatin, are responsible for the observed cell cycle pattern of in vivo histone acetylation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Golderer G,Loidl P,Gröbner Pdoi
10.1016/0014-5793(87)80394-0subject
Has Abstractpub_date
1987-10-05 00:00:00pages
322-6issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(87)80394-0journal_volume
222pub_type
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