Abstract:
:The integrin alpha IIb beta 3-mediated redistribution of the tyrosine kinases pp125FAK and pp60Src and the small GTP-binding proteins CDC42Hs and Rap1B from the membrane skeleton to the cytoskeleton was found to be reversible: upon prolonged platelet aggregation (up to 15 min) induced by the thrombin-receptor activating peptide (TRAP) these signalling proteins dissociated from the cytoskeleton and reappeared in the membrane skeleton. Addition of the extracellular Ca2+ chelator EGTA and the intracellular Ca2+ chelator BAPTA/AM 30 s after TRAP allowed platelet aggregation and the association of pp125FAK, pp60Src, CDC42Hs and Rap1B with the cytoskeleton, but prevented their dissociation from the cytoskeleton. The results indicate that the prolonged elevation of cytosolic Ca2+ in stimulated platelets leads to the dissociation of signalling proteins from the cytoskeleton.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Dash D,Aepfelbacher M,Siess Wdoi
10.1016/0014-5793(95)00320-9subject
Has Abstractpub_date
1995-04-24 00:00:00pages
231-4issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(95)00320-9journal_volume
363pub_type
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