Abstract:
:A simple technique for rapid isolation of mitochondrial DNA (mtDNA) from animal cells is described. The method is based on the selective alkaline denaturation procedure of Birnboim and Doly [(1979) Nucleic Acids Res. 7, 1513-1523] and avoids the use of CsCl gradient centrifugation. The yield of mtDNA is comparable to that obtained by standard techniques. This DNA is sufficiently pure for restriction analysis and cloning of mtDNA fragments.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Palva TK,Palva ETdoi
10.1016/0014-5793(85)80122-8subject
Has Abstractpub_date
1985-11-18 00:00:00pages
267-70issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(85)80122-8journal_volume
192pub_type
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