Abstract:
:The dynamics of chromatin structure are tightly regulated by multiple epigenetic mechanisms such as histone modifications and incorporation of histone variants. In the current work, differentiation of an embryonal carcinoma cell line, NT2, was induced by retinoic acid, and total histone proteins were compared throughout this process. The results showed a significant change in expression level of a variant of H1 histone named H1x. Chromatin immunoprecipitation coupled with real-time PCR analysis demonstrated a preferential incorporation of this protein in the regulatory region of Nanog, a marker gene of stemness that is significantly suppressed in differentiated cells. This finding reveals a dynamic role of H1x in differentiation, and implies a repressive role for this histone variant.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Shahhoseini M,Favaedi R,Baharvand H,Sharma V,Stunnenberg HGdoi
10.1016/j.febslet.2010.10.041subject
Has Abstractpub_date
2010-11-19 00:00:00pages
4661-4issue
22eissn
0014-5793issn
1873-3468pii
S0014-5793(10)00857-4journal_volume
584pub_type
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