Involvement of AMP-activated protein kinase in fat depot-specific metabolic changes during starvation.

abstract：:Insolubility in non-ionic detergents such as Triton X-100 is a widely used biochemical criterion for characterization of membrane domains. We report here a novel green fluorescent protein fluorescence-based approach to directly determine detergent insolubility of specific membrane proteins. We have applied this method...

journal_title：FEBS letters

authors： Kalipatnapu S,Chattopadhyay A

更新日期：2004-10-22 00:00:00

abstract：:The nuclear gene OXA1 was first isolated in Saccharomyces cerevisiae and found to be required at a post-translational step in cytochrome c oxidase biogenesis, probably at the level of assembly. Mutations in OXA1 lead to a complete respiratory deficiency. The protein Oxa1p is conserved through evolution and a human hom...

journal_title：FEBS letters

authors： Altamura N,Capitanio N,Bonnefoy N,Papa S,Dujardin G

更新日期：1996-03-11 00:00:00

abstract：:We report a nonenzymatic reaction which results in the radiolabeling of proteins by 32P-containing nucleoside triphosphates. The labeling reaction does not require any cofactors, but is greatly enhanced by the presence of alcohols. Even under optimal conditions, less than 1% of the protein molecules undergo modificati...

journal_title：FEBS letters

authors： Schmidt MC,Hanna MM

更新日期：1986-01-06 00:00:00

abstract：:TLR3 and TLR4 utilize adaptor TRIF to activate interferon regulatory factor 3 (IRF3), resulting in interferon β (IFN-β) production to mediate anti-viral infection. In this report, we analyzed the effect of two known phosphatidylinositol 3-kinase (PI3K) inhibitors LY294002 and wortmannin on LPS- and poly(I:C)-induced I...

journal_title：FEBS letters

authors： Zhao W,Qi J,Wang L,Zhang M,Wang P,Gao C

更新日期：2012-03-23 00:00:00

abstract：:Mice are widely used for exploring obesity physiology and treatment. However, thermal biology is different between small and large mammals. In this Review, we discuss how the effect of environmental temperature must be understood to ensure applicability of mouse experiments to human obesity. At ambient environmental t...

journal_title：FEBS letters

authors： Reitman ML

更新日期：2018-06-01 00:00:00

abstract：:Treatment of neonatal rat islets of Langerhans with combined cytokines (interleukin-1beta 10(-10) M, tumour necrosis factor-alpha 10(-10) M, interferon-gamma 5 U/ml) led to extensive cell death, which was potentiated by Fas activation with the anti-Fas cytolytic antibody JO2. Pre-treatment with insulin (25 ng/ml) or i...

journal_title：FEBS letters

authors： Harrison M,Dunger AM,Berg S,Mabley J,John N,Green MH,Green IC

更新日期：1998-09-18 00:00:00

abstract：:We report here the synthesis of novel 5'-linked oligodeoxynucleotides, both normal phosphodiester and phosphorothioate analogs, in which a covalently attached group at the 5'-terminus is an anthraquinone. These compounds represent a new class of antisense compounds in which the base sequence of the oligodeoxynucleotid...

journal_title：FEBS letters

authors： Mori K,Subasinghe C,Cohen JS

更新日期：1989-06-05 00:00:00

abstract：:Energetic behaviors of eight pairs of RNA/DNA hybrid duplexes with identical nearest neighbors have been investigated by UV melting analysis. In the pairs with identical nearest-neighbor pairs, the melting curve traces at the same strand concentration were very similar. The average difference in stabilization energy o...

journal_title：FEBS letters

authors： Sugimoto N,Katoh M,Nakano S,Ohmichi T,Sasaki M

更新日期：1994-10-31 00:00:00

abstract：:A possible interaction between tumor necrosis factor-alpha (TNF) and other cytokines/growth factors in stimulating the production of nerve growth factor (NGF) in Swiss 3T3 cells was studied. TNF's stimulatory activity on fibroblast NGF production was synergized by interleukin-1 alpha (IL-1 alpha), IL-1 beta and interf...

journal_title：FEBS letters

authors： Hattori A,Iwasaki S,Murase K,Tsujimoto M,Sato M,Hayashi K,Kohno M

更新日期：1994-03-07 00:00:00

abstract：:The odorant-induced accumulation of cAMP can be inhibited by antibodies directed against G alpha s/olf. In contrast, antibodies raised against G alpha i-subunits caused a strong enhancement of the odorant-induced cAMP accumulation. Western blotting and immunoelectron microscopy revealed the presence of both G alpha s/...

journal_title：FEBS letters

authors： Sinnarajah S,Ezeh PI,Pathirana S,Moss AG,Morrison EE,Vodyanoy V

更新日期：1998-04-24 00:00:00

abstract：:The relationship among the real-time trafficking of lung metastatic B16BL6 cells, metastatic potential, and the injected number of the cells was examined, since the smaller the number of tumor cells injected, the more clearly the immune defense may be observed. When 1x10(6) or 1x10(5) B16BL6 cells were injected into m...

journal_title：FEBS letters

authors： Kikkawa H,Imafuku H,Tsukada H,Oku N

更新日期：2000-02-11 00:00:00

abstract：:The farnesoid X receptor (FXR) is a nuclear receptor activated by bile acids (BAs). In response to ligand-binding, FXR regulates many genes involved in BA, lipid, and lipoprotein metabolism. To identify new FXR target genes, microarray technology was used to profile total RNA extracted from HepG2 cells treated with th...

journal_title：FEBS letters

authors： Sirvent A,Claudel T,Martin G,Brozek J,Kosykh V,Darteil R,Hum DW,Fruchart JC,Staels B

更新日期：2004-05-21 00:00:00

abstract：:Plant G proteins of the ROP/RAC family regulate cellular processes including cytoskeletal rearrangement in polar growth. Activation of the ROP molecular switch is triggered by guanine nucleotide exchange factors. Plant-specific RopGEFs are exclusively active on ROPs despite their high homology to animal Rho proteins. ...

journal_title：FEBS letters

authors： Fricke I,Berken A

更新日期：2009-01-05 00:00:00

abstract：:Proteases not only play a fundamental role in numerous physiological processes, but are also involved in several human diseases including Alzheimer's disease (AD). A key protease implicated in AD is the so far unidentified gamma-secretase, which cleaves the membrane-bound beta-amyloid precursor protein (betaAPP) at th...

journal_title：FEBS letters

authors： Steiner H,Pesold B,Haass C

更新日期：1999-12-17 00:00:00

abstract：:Laminin 5, an anchoring filament protein previously known as nicein/kalinin/epiligrin, consists of three polypeptide chains, alpha 3, beta 3, and gamma 2, encoded by the genes LAMA3, LAMB3, and LAMC2, respectively. The expression of laminin 5 was detected by Northern hybridization with specific cDNA probes in various ...

journal_title：FEBS letters

authors： Korang K,Christiano AM,Uitto J,Mauviel A

更新日期：1995-07-24 00:00:00

abstract：:Lipid analyses were carried out on transplantable murine Friend leukemia cell tumors, 6 h after intratumoral administration of tumor necrosis factor-alpha (TNF). The levels of the major phospholipid classes were uniformly decreased to about 70% of control values; free fatty acids were increased to about 170%; diacylgl...

journal_title：FEBS letters

authors： Bravo E,Carpinelli G,Proietti E,Belardelli F,Cantafora A,Podo F

更新日期：1990-01-29 00:00:00

abstract：:The regulatory system of smooth muscle thin filaments is thought to involve a major calcium-calmodulin and actin binding protein: caldesmon. A dissective approach was used to isolate a 35 kDa C-terminal fragment of the molecule and to produce antibodies reacting against both the intact and the 15 kDa N-terminal end of...

journal_title：FEBS letters

authors： Harricane MC,Cavadore C,Audemard E,Mornet D

更新日期：1990-08-20 00:00:00

abstract：:The human DNA-dependent activator of IFN-regulatory factor (DAI) protein, which activates the innate immune response in response to DNA, contains two tandem Z-DNA binding domains (Zα and Zβ) at the NH(2) terminus. The hZβ(DAI) structure is similar to other Z-DNA binding proteins, although it demonstrates an unusual Z-...

journal_title：FEBS letters

authors： Kim HE,Ahn HC,Lee YM,Lee EH,Seo YJ,Kim YG,Kim KK,Choi BS,Lee JH

更新日期：2011-03-09 00:00:00

abstract：:This study describes the comparative analysis of two insect recombinant aminotransferases, Aedes aegypti 3-hydroxykynurenine (3-HK) transaminase/alanine glyoxylate aminotransferase (Ae-HKT/AGT) and Drosophila melanogaster serine pyruvate aminotransferase (Dm-Spat), which share 52% identity in their amino acid sequence...

journal_title：FEBS letters

authors： Han Q,Li J

更新日期：2002-09-11 00:00:00

abstract：:A major house dust mite allergen Der f 1 belongs to the papain-like cysteine protease family. This study investigated whether Der f 1 can cleave the latency-associated peptide (LAP) of transforming growth factor (TGF)-beta via its proteolytic activity and activate latent TGF-beta. We found that Der f 1 can cleave LAP ...

journal_title：FEBS letters

authors： Nakamura Y,Miyata M,Shimokawa N,Ohnuma Y,Katoh R,Ogawa H,Okumura K,Nakao A

更新日期：2009-06-18 00:00:00

abstract：:The complexity of the proteasome gene family in higher plants was investigated by identification and sequencing cDNA clones from the Arabidopsis thaliana database showing homologies to 20S proteasome subunits. We identified plant counterparts for each of the 14 proteasomal subunit subfamilies. Moreover, several of the...

journal_title：FEBS letters

authors： Parmentier Y,Bouchez D,Fleck J,Genschik P

更新日期：1997-10-27 00:00:00

abstract：:Exposure of protein kinase C to low concentrations of either N-chlorosuccinimide or H2O2 resulted in rapid and parallel loss of phosphotransferase activity and phorbol ester binding. This oxidative inactivation of protein kinase C also occurred in intact cells exposed to a low concentration of H2O2. With H2O2 treatmen...

journal_title：FEBS letters

authors： Gopalakrishna R,Anderson WB

更新日期：1987-12-10 00:00:00

abstract：:Di(1,N6-ethenoadenosine)5',5'''-P1,P4-tetraphosphate, epsilon-(Ap4A), a fluorescent analog of Ap4A has been synthesized by reaction of 2-chloroacetaldehyde with Ap4A. At neutral pH this Ap4A analog presents characteristics maxima at 265 and 274 nm, shoulders at ca 260 and 310 nm and moderate fluorescence (lambda exc 3...

journal_title：FEBS letters

authors： Rotilán P,Ramos A,Pintor J,Torres M,Miras-Portugal MT

更新日期：1991-03-25 00:00:00

abstract：:By sequence analysis we show that the U104 domain found in the UNC104 subfamily of kinesins is a forkhead homology-associated domain (FHA). A combination of limited proteolysis, mass spectroscopy, and physicochemical analysis define this domain as a genuine autonomously folding domain. Our data show that the FHA domai...

journal_title：FEBS letters

authors： Westerholm-Parvinen A,Vernos I,Serrano L

更新日期：2000-12-15 00:00:00

abstract：:The functional molecular masses of the vacuolar membrane H+-ATPase in Saccharomyces cerevisiae under two kinetic conditions for ATP hydrolysis were measured by radiation inactivation. When vacuolar membrane vesicles were exposed to gamma-rays from 60Co, the activities catalyzing a single-cycle and multi-cycles of ATP ...

journal_title：FEBS letters

authors： Hirata R,Ohsumi Y,Anraku Y

更新日期：1989-02-27 00:00:00

abstract：:N-Oligosaccharyltransferase catalyzes the N-glycosylation of asparagine residues of nascent polypeptide chains in the endoplasmic reticulum, a pathway highly conserved in all eukaryotes. An enzymatically active complex was isolated from microsomal membranes from Saccharomyces cerevisiae, which is composed of four prot...

journal_title：FEBS letters

authors： Knauer R,Lehle L

更新日期：1994-05-09 00:00:00

abstract：:Programmed death-ligand 1 (PD-L1) is a co-inhibitory molecule expressed on tumor cells. Immune checkpoint inhibitors focusing on the PD-L1 mechanism are now being studied for the treatment of various cancer types. However, the regulatory mechanism of PD-L1 is yet to be fully clarified, and a high-throughput system for...

journal_title：FEBS letters

authors： Uchida Y,Matsushima T,Kurimoto R,Chiba T,Inutani Y,Asahara H

更新日期：2021-01-09 00:00:00

abstract：:Intrinsically unstructured proteins (IUPs) are common in various proteomes and occupy a unique structural and functional niche in which function is directly linked to structural disorder. The evidence that these proteins exist without a well-defined folded structure in vitro is compelling, and justifies considering th...

journal_title：FEBS letters

authors： Tompa P

更新日期：2005-06-13 00:00:00

abstract：:The isolation and primary structure of intestinal neuropeptide Y (NPY) is described. The peptide was purified from porcine intestinal extracts using a chemical assay and radioimmunoassay for NPY. The amino acid sequence of this peptide is: Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Ala-Glu-Asp-Leu-Ala- Arg-Ty...

journal_title：FEBS letters

authors： Tatemoto K,Siimesmaa S,Jörnvall H,Allen JM,Polak JM,Bloom SR,Mutt V

更新日期：1985-01-01 00:00:00

abstract：:The tissue inhibitors of matrix metalloproteinases (MMPs), TIMP-1 and TIMP-2, are also angiogenesis inhibitors. Cathepsin B and MMPs are found at sites of neovascularization in pathologies such as cancer and osteoarthritis. Treatment of TIMP-1, TIMP-2, and of a mixture of both inhibitors from human articular chondrocy...

journal_title：FEBS letters

authors： Kostoulas G,Lang A,Nagase H,Baici A

更新日期：1999-07-23 00:00:00