Abstract:
:Programmed death-ligand 1 (PD-L1) is a co-inhibitory molecule expressed on tumor cells. Immune checkpoint inhibitors focusing on the PD-L1 mechanism are now being studied for the treatment of various cancer types. However, the regulatory mechanism of PD-L1 is yet to be fully clarified, and a high-throughput system for comparing the abilities of small compounds in regulating PD-L1 has not yet been established. Therefore, we created a HiBiT-tagged lung adenocarcinoma cell line, PC9-KI, for easier and faster detection of changes in PD-L1 protein expression. Using PC9-KI cells, we screened 1280 chemical compounds from the Library of Pharmacologically Active Compounds and identified microtubule polymerization inhibitors and thapsigargin as PD-L1 upregulators and a p97 inhibitor as a PD-L1 downregulator.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Uchida Y,Matsushima T,Kurimoto R,Chiba T,Inutani Y,Asahara Hdoi
10.1002/1873-3468.14032subject
Has Abstractpub_date
2021-01-09 00:00:00eissn
0014-5793issn
1873-3468pub_type
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