Abstract:
:Energetic behaviors of eight pairs of RNA/DNA hybrid duplexes with identical nearest neighbors have been investigated by UV melting analysis. In the pairs with identical nearest-neighbor pairs, the melting curve traces at the same strand concentration were very similar. The average difference in stabilization energy of these pairs was 4%, which was about expected within experimental error. These results indicate that the nearest-neighbor model is valid for predicting the stability of RNA/DNA hybrid duplexes as well as RNA/RNA and DNA/DNA duplexes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sugimoto N,Katoh M,Nakano S,Ohmichi T,Sasaki Mdoi
10.1016/0014-5793(94)01098-6subject
Has Abstractpub_date
1994-10-31 00:00:00pages
74-8issue
1eissn
0014-5793issn
1873-3468journal_volume
354pub_type
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