RNA/DNA hybrid duplexes with identical nearest-neighbor base-pairs have identical stability.

Abstract:

:Energetic behaviors of eight pairs of RNA/DNA hybrid duplexes with identical nearest neighbors have been investigated by UV melting analysis. In the pairs with identical nearest-neighbor pairs, the melting curve traces at the same strand concentration were very similar. The average difference in stabilization energy of these pairs was 4%, which was about expected within experimental error. These results indicate that the nearest-neighbor model is valid for predicting the stability of RNA/DNA hybrid duplexes as well as RNA/RNA and DNA/DNA duplexes.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Sugimoto N,Katoh M,Nakano S,Ohmichi T,Sasaki M

doi

10.1016/0014-5793(94)01098-6

subject

Has Abstract

pub_date

1994-10-31 00:00:00

pages

74-8

issue

1

eissn

0014-5793

issn

1873-3468

journal_volume

354

pub_type

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