Abstract:
:N-Oligosaccharyltransferase catalyzes the N-glycosylation of asparagine residues of nascent polypeptide chains in the endoplasmic reticulum, a pathway highly conserved in all eukaryotes. An enzymatically active complex was isolated from microsomal membranes from Saccharomyces cerevisiae, which is composed of four proteins: Wbp1p and Swp1p (previously found to be encoded by two essential genes necessary for N-glycosylation in vivo and in vitro) and two additional proteins with a molecular mass of 60/62 kDa and 34 kDa. The 60/62 component represents differentially glycosylated forms of a protein that has sequence homology to ribophorin I. Wbp1p and Swp1p reveal homology to mammalian OST 48 and ribophorin II, respectively. Ribophorin I and II and OST 48 were recently shown to be constituents of the mammalian transferase from dog pancreas. The data reveal a high conservation of the organization of this enzyme activity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Knauer R,Lehle Ldoi
10.1016/0014-5793(94)00356-4subject
Has Abstractpub_date
1994-05-09 00:00:00pages
83-6issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(94)00356-4journal_volume
344pub_type
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