Abstract:
:RCR cells are NRK clones in which Raf-1 production is blocked by the expression of an antisense RNA, and consequently they are refractory to transformation by various oncogenes. In RCR cells, MAP kinases (ERK1 and ERK2) were activated to an extent and in a time course similar to those of the original NRK cells, irrespective of whether the stimulus was oncogenic or non-oncogenic. Moreover, there was no significant elevation of ERK activities in oncogene-transformed NRK cells. These results indicate that Raf-1 kinase is not the major upstream activator of ERK's in NRK cells and that neither ERK1 nor ERK2 are likely to mediate oncogenic signals from Raf-1 kinase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kizaka-Kondoh S,Okayama Hdoi
10.1016/0014-5793(93)80814-bsubject
Has Abstractpub_date
1993-12-27 00:00:00pages
255-8issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(93)80814-Bjournal_volume
336pub_type
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