Abstract:
:The receptor structure of Clostridium botulinum neurotoxin type A was analysed by TLC immunostaining. GQ1b was found to be the most potent receptor, and the neurotoxin also bound to GT1b and GD1a, but not to GM3, GM2, GM1, GD3, GD1b and GT1a. Optimum binding of neurotoxin to the ganglioside appeared in 0.01 M phosphate buffer (pH 7.2) containing 0.2% NaCl. Higher and lower NaCl concentrations diminished neurotoxin binding to the ganglioside. In addition, the neurotoxin was able to bind to free fatty acids. Maximum binding was observed on stearic acid and neurotoxin binding to free fatty acids was not affected by NaCl concentration.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Takamizawa K,Iwamori M,Kozaki S,Sakaguchi G,Tanaka R,Takayama H,Nagai Ydoi
10.1016/0014-5793(86)80614-7subject
Has Abstractpub_date
1986-06-09 00:00:00pages
229-32issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(86)80614-7journal_volume
201pub_type
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