Abstract:
:The spatial relationship of polypeptides comprising the light-harvesting B800-850 complex of Chromatium minutissimum has been studied by means of chemical crosslinking of the isolated complex with cleavable, 1.2 nm-long dithiobis(succinimidyl propionate). The samples were analyzed by different types of electrophoresis and spectrophotometrically. No difference was shown between crosslinking of the B800-850 complex either solubilized or incorporated into proteoliposomes. It was found that two main polypeptides form only one type of heterodimer. The crosslinked complex was more thermostable. This crosslinkage restricted the conformational transitions causing the shift of the long wavelength band in the near infrared region. A structure of the complex is discussed.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Moskalenko AA,Toropygina OAdoi
10.1016/0014-5793(93)81650-osubject
Has Abstractpub_date
1993-03-29 00:00:00pages
28-30issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(93)81650-Ojournal_volume
320pub_type
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