Abstract:
:Calf thymus (ct) Hsc70 has been shown previously to reactivate heat-inactivated prokaryotic and eukaryotic enzymes, while DnaK was able to reactivate solely prokaryotic enzymes. Here, we report on isolation from calf thymus of a DnaJ homolog, ctHsc40, and on testing of its cooperative function in three different assays: (i) reactivation of heat-inactivated DNA polymerases, (ii) stimulation of the ATPase activity of ctHsc70 chaperone, and (iii) replication of bacteriophage lambda DNA. Surprisingly, ctHsc70/ctHsc40 chaperones were found to reactivate the denatured prokaryotic and eukaryotic enzymes but not to promote bacteriophage lambda DNA replication, suggesting species specificity in DNA replication.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ziemienowicz A,Konieczny I,Hübscher Udoi
10.1016/s0014-5793(01)02923-4keywords:
subject
Has Abstractpub_date
2001-10-19 00:00:00pages
11-5issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(01)02923-4journal_volume
507pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::In blood-borne metastasis, intravasated metastatic tumor cells are thought to localize at the target site via a series of processes involving platelet aggregation, adhesion to endothelium, and invasion through the basal membrane. In the present study, we examined how platelet aggregation contributes to the trafficking...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00441-4
更新日期:1998-05-08 00:00:00
abstract::The chaotropic character of several ions determines their partition on membrane interfaces with aqueous media as predicted by the Hofmeister series. However, specific characteristics of each individual ion determine its ability to cross the membrane and to influence Ca2+, H+ and electrical gradients produced by the sa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80948-t
更新日期:1993-08-16 00:00:00
abstract::Interactions between Trypanosoma cruzi and the extracellular matrix play an important role in cellular invasion. Here we show that T. cruzi increases the levels of thrombospondin-1 (TSP-1) expression in host cells during early infection. Stable RNA interference of host cell TSP-1 knocks down the levels of TSP-1 transc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.03.054
更新日期:2006-04-17 00:00:00
abstract::In Drosophila, the tumor suppressor Scribble is localized at the septate junctions of epithelial cells. Its mammalian homologue, hScrib, is a basolateral protein likely associated to proteins of the cell-cell junctions. We report the direct interaction between hScrib and ZO-2, a junction-associated protein. This inter...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.05.062
更新日期:2005-07-04 00:00:00
abstract::MRI has the ability to generate high-contrast and high-resolution images, to obtain multiple diagnostic evaluations of organ function and morphology, and to provide multiple image planes with no risk of ionizing radiation. Recent efforts have focused on using MR technology to monitor gene delivery, to enhance gene tra...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2006.04.027
更新日期:2006-05-22 00:00:00
abstract::Gibberellins are ent-kaurene-derived diterpenoid phytohormones produced by plants, fungi, and bacteria. The distinct gibberellin biosynthetic pathways in plants and fungi are known, but not that in bacteria. Plants typically use two diterpene synthases to form ent-kaurene, while fungi use only a single bifunctional di...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.12.052
更新日期:2009-01-22 00:00:00
abstract::Hemoglobin-binding proteins are necessary for pathogens to obtain iron from Hb. Entamoeba histolytica can grow using Hb as source of iron, but the underlying mechanism has not previously been established. In this work, we identified a 45 kDa Hb-binding protein of E. histolytica, which we named Ehhmbp45. In silico anal...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.06.057
更新日期:2008-08-06 00:00:00
abstract::Gustatory papillae and associated taste buds receive and process chemical information from the environment. In mammals, their development takes place during the late phase of embryogenesis. However, the cellular factors that regulate the differentiation of taste papillae remain largely unknown. Here, we show by quanti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.08.078
更新日期:2006-10-02 00:00:00
abstract::Free radicals, including dopamine (DA)-oxidized metabolites, have long been implicated in pathogenesis of Parkinson's disease (PD). However, the relationships between such oxidative stresses and alpha-synuclein (alpha-S), a major constituent of Lewy bodies, remain unknown. In this study, we established neuronal cells ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.03.018
更新日期:2006-04-03 00:00:00
abstract::Vascular endothelial growth factor (VEGF) mRNA expression was analysed in rabbit vascular smooth muscle cells following exposure to hypoxia and platelet-derived growth factor-BB (PDGF-BB). Hypoxia potently upregulated VEGF mRNA steady-state levels in a time- and concentration-dependent manner reaching a maximum level ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01458-d
更新日期:1995-01-30 00:00:00
abstract::Gene duplication provides genetic material required for functional diversification. An interesting example is the amyloid precursor protein (APP) protein family. The APP gene family has experienced both expansion and contraction during evolution. The three mammalian members have been studied quite extensively in combi...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2013.05.026
更新日期:2013-06-27 00:00:00
abstract::The H-Ras-like suppressor (HRASLS) is a protein family consisting of five members in humans. Despite their discovery as tumor suppressors, we demonstrated that all these proteins are phospholipid-metabolizing enzymes, such as phospholipase (PL) A1 /A2 and acyltransferase. We thus proposed to rename HRASLS1-5 as PLA/ac...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12787
更新日期:2017-09-01 00:00:00
abstract::We have previously generated transgenic (Tg) mice expressing the human apolipoprotein (apo) A-I/C-III/A-IV gene cluster. This expression induced hyperlipidemia but reduced atherosclerotic lesions in genetically modified mice lacking apoE. Atherosclerosis is a multifactorial process and environmental factors such as di...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02621-7
更新日期:2001-07-27 00:00:00
abstract::Chimeric proteins composed of ricin toxin A chain (RTA) and staphylococcal protein A (PA) have been produced in E. coli. Constructs consisting of N-terminal RTA and C-terminal PA (RTA-PA) or N-terminal PA and C-terminal (PA-RTA) were capable of binding to immunoglobulin G (via PA) and of specifically depurinating 28 S...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81084-2
更新日期:1990-10-29 00:00:00
abstract::A cDNA clone encoding cytochrome P-450(11)beta of rat adrenal has been cloned and sequenced using a bovine P-450(11)beta cDNA insert (pcP-450(11 beta)-2; (1987) J. Biochem. 102, 559-568) as a probe. The nucleotide sequence contains an open reading frame sufficient to encode the entire amino acid sequence of a P-450(11...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81053-1
更新日期:1989-09-11 00:00:00
abstract::The photoreduction of the primary electron acceptor, QA, has been characterized by light-induced Fourier transform infrared difference spectroscopy for Rb. sphaeroides reaction centers and for Rsp. rubrum and Rp. viridis chromatophores. The samples were treated both with redox compounds, which rapidly reduce the photo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80129-q
更新日期:1991-01-28 00:00:00
abstract::In Gram-positive bacteria the tRNA-dependent T box riboswitch regulates the expression of many amino acid biosynthetic and aminoacyl-tRNA synthetase genes through a transcription attenuation mechanism. The Specifier domain of the T box riboswitch contains the Specifier sequence that is complementary to the tRNA antico...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.09.003
更新日期:2013-11-01 00:00:00
abstract::Mona is an SH3 and SH2 domain-containing adapter molecule that is induced during monocytic differentiation. Here we have first shown that M-CSFR is the major Mona partner in M-CSF signaling, the interaction being mediated through tyrosine 697 of the receptor. Next we asked whether Mona expression would alter the Ras/M...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01906-2
更新日期:2000-09-01 00:00:00
abstract::The complete amino acid sequence of human plasma apolipoprotein C-III (apoC-III) isolated from normal subjects is described. ApoC-III is a linear polypeptide chain of 79 amino acids. Tryptic digestion of intact apoC-III produced 5 major peptides, while tryptic digestion of the citraconylated protein yielded two peptid...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80300-3
更新日期:1986-03-03 00:00:00
abstract::Matrix metalloproteinase-13 (MMP-13) plays a critical role in parathyroid hormone (PTH)-induced bone resorption. PTH acts via protein kinase A (PKA) to phosphorylate and stimulate the transactivation of Runx2 for MMP-13 promoter activation. We show here that PTH stimulated Runx2 phosphorylation in rat osteoblastic cel...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.02.040
更新日期:2009-04-02 00:00:00
abstract::We examined the spatial expression patterns of two orphan receptor guanylyl cyclase genes OlGC4 and OlGC5 during embryogenesis of medaka and characterized the 5' flanking region required for tissue-specific expression of OlGC4 by introducing promoter-GFP fusion constructs into medaka embryos. Expression of OlGC5 is co...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02109-8
更新日期:2000-10-20 00:00:00
abstract::Bovine globin has been incubated with mice peritoneal macrophages in order to study its hydrolysis by lysosomal enzymes, among which chiefly cathepsin D. Analysis of resulting peptides, by reversed-phase high-performance liquid chromatography (RP-HPLC), showed the release of a bioactive peptide, VV-hemorphin-7. When a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00144-5
更新日期:1996-03-11 00:00:00
abstract::Replacements of Asp-295 by Asn (D295N) and Glu (D295E) decreased the catalytic center activity of Leuconostoc mesenteroides sucrose phosphorylase to about 0.01% of the wild-type level (k(cat)=200s(-1)). Glucosylation and deglucosylation steps of D295N were affected uniformly, approximately 10(4.3)-fold, and independen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.02.060
更新日期:2007-04-03 00:00:00
abstract::In endocrine cells the regulated secretion of hormones, peptides, enzymes and neurotransmitters into the external medium occurs when mature secretory granules fuse with the plasma membrane. Secretory granules form at the trans-Golgi network (TGN) by envelopment of the dense-core aggregate of regulated secretory protei...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(91)80805-d
更新日期:1991-07-22 00:00:00
abstract::The bcl-2 gene is an important antagonist of apoptosis, the programmed cell death. Bcl-2 is highly expressed in a variety of lymphomas. Lymphocytes of patients with chronic lymphocytic leukemia (CLL) express high amounts of bcl-2 even in the absence of the t(14;18) translocation, resulting in a strong resistance towar...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00229-9
更新日期:1997-04-07 00:00:00
abstract::Calcium channels derived from sarcoplasmic reticulum of frog skeletal muscle were fused with planar lipid bilayers. Fractional open times displayed two types of calcium dependence: (i) blockable channels showed a bell-shaped calcium dependence with an activation constant of 4.5 microM, a Hill coefficient for activatio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80341-q
更新日期:1993-10-04 00:00:00
abstract::Temperature-induced order/disorder transition profiles were obtained from the membranes of intact Gram-negative bacterial cells by FT-IR analysis of the frequency shifts of the acyl chain methylene symmetric stretching band as a monitor. Cells grown at different temperatures yielded distinct transition profiles. At th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81339-a
更新日期:1991-12-02 00:00:00
abstract::We have used DNase I footprinting to examine the effect of a novel naphthylquinoline dimer, designed as a triplex-specific bis-intercalator, on the stability of intermolecular DNA triplexes. We find that this compound efficiently promotes triplex formation between the 9-mer oligonucleotide 5'-TTTTTTCTT and its oligopu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00284-7
更新日期:1999-03-26 00:00:00
abstract::There are numerous examples of how fundamental research has been required to understand and treat human disease. This article focuses on three human diseases of lipid metabolism in which advancements in understanding and treatment would not have been possible without basic research. Fabry disease is an inherited metab...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2006.06.025
更新日期:2006-10-09 00:00:00
abstract::Cyclic AMP (cAMP) and its action by way of cAMP-dependent protein kinase is important for sperm motility. Previous studies on germ cells have demonstrated a selective decrease in the amount of type I cAMP-dependent protein kinase during spermatid development, and that type II was the major form present in elongating s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81163-3
更新日期:1988-03-14 00:00:00