A unique mRNA species for a regulatory subunit of cAMP-dependent protein kinase is specifically induced in haploid germ cells.

abstract：:Rab proteins are small GTPases involved in the regulation of membrane traffic. Rab5a has been shown to regulate transport in the early endocytic pathway. Here we report the isolation of cDNA clones encoding two highly related isoforms, Rab5b and Rab5c. The two proteins share with Rab5a all the structural features requ...

journal_title：FEBS letters

authors： Bucci C,Lütcke A,Steele-Mortimer O,Olkkonen VM,Dupree P,Chiariello M,Bruni CB,Simons K,Zerial M

更新日期：1995-06-05 00:00:00

abstract：:Recombinant adipogenesis inhibitory factor (AGIF) was purified to homogeneity from the conditioned medium of COS-1 cells transfected with human AGIF cDNA. The amino-terminal sequence analysis of the mature AGIF revealed that AGIF was produced as a precursor consisting of 199 amino acids and processed into a mature for...

journal_title：FEBS letters

authors： Ohsumi J,Miyadai K,Kawashima I,Ishikawa-Ohsumi H,Sakakibara S,Mita-Honjo K,Takiguchi Y

更新日期：1991-08-19 00:00:00

abstract：:Extracellular laccase (benzenediol: oxygen oxidoreductase EC 1.10.3.2) from the lignin-degrading fungus, Phlebia radiata, was shown to contain a novel combination of electron carriers as its prosthetic groups. In addition to two copper atoms per enzyme molecule, one molecule of PQQ was included as a cofactor. The EPR ...

journal_title：FEBS letters

authors： Karhunen E,Niku-Paavola ML,Viikari L,Haltia T,van der Meer RA,Duine JA

更新日期：1990-07-02 00:00:00

abstract：:We have purified a form of protein phosphatase 1 (PP1) from HeLa cell nuclei, in which the phosphatase is complexed to a regulatory subunit termed p99. We report here the cloning and characterisation of the p99 component. p99 mRNA is widely expressed in human tissues and immunofluorescence analysis with anti-p99 antib...

journal_title：FEBS letters

authors： Kreivi JP,Trinkle-Mulcahy L,Lyon CE,Morrice NA,Cohen P,Lamond AI

更新日期：1997-12-22 00:00:00

abstract：:When a high density of alpha1-subunit glycine receptor (GlyR) is expressed in Xenopus oocytes, two populations of channels can be distinguished according to their apparent affinity for glycine which differs 5- to 6-fold. To compare the open pore diameter of these channels, the relative permeability of formate with res...

journal_title：FEBS letters

authors： Maammar M,Rodeau JL,Taleb O

更新日期：1997-09-01 00:00:00

abstract：:The farnesoid X receptor (FXR) is a member of the nuclear receptor superfamily that is primarily expressed in the enterohepatic system where it functions as intracellular sensor for bile acids. Ligand dependent FXR activation induces transcriptional responses to coordinately regulate bile acid, cholesterol, triglyceri...

journal_title：FEBS letters

authors： Modica S,Moschetta A

更新日期：2006-10-09 00:00:00

abstract：:Synaptotagmin I, the calcium sensor for neurotransmission, is palmitoylated. We have identified the palmitoylation sites as five cysteine residues located between the transmembrane and cytoplasmic regions. In contrast to wild-type synaptotagmin, the non-acylated mutant is not converted to the endoglycosidase-H-resista...

journal_title：FEBS letters

authors： Heindel U,Schmidt MF,Veit M

更新日期：2003-06-05 00:00:00

abstract：:A new 175-kDa membrane protein was isolated from chicken gizzard smooth muscle. Antibodies to 175-kDa protein were used for localization of this protein in smooth and cardiac muscles. In both types of muscle 175-kDa protein was localized near plasma membrane. 175-kDa protein was able to interact specifically with vinc...

journal_title：FEBS letters

authors： Koteliansky VE,Gneushev GN,Belkin AM

更新日期：1985-03-11 00:00:00

abstract：:Bacterial fructosyltransferase enzymes belonging to glycoside hydrolase family 68 (GH68) are not known to require a metal cofactor. Here, we show that Ca2+ ions play an important structural role in the Lactobacillus reuteri 121 levansucrase (Lev) and inulosucrase (Inu) enzymes. Analysis of the Bacillus subtilis Lev 3D...

journal_title：FEBS letters

authors： Ozimek LK,Euverink GJ,van der Maarel MJ,Dijkhuizen L

更新日期：2005-02-14 00:00:00

abstract：:Under destabilising conditions both heat and cold denaturation of yeast phosphoglycerate kinase (PGK) can be observed. According to previous interpretation of experimental data and theoretical calculations, the C-terminal domain should be more stable than the N-terminal domain at all temperatures. We report on thermal...

journal_title：FEBS letters

authors： Gast K,Damaschun G,Desmadril M,Minard P,Müller-Frohne M,Pfeil W,Zirwer D

更新日期：1995-01-30 00:00:00

abstract：:A number of human diseases can be linked to aberrations in protein folding which cause an imbalance in protein homeostasis. Molecular chaperones, including heat shock proteins, act to assist protein folding, stability and activity in the cell. Attention has begun to focus on modulating the expression and/or activity o...

journal_title：FEBS letters

authors： Powers MV,Workman P

更新日期：2007-07-31 00:00:00

abstract：:The cleavable prepiece of the precursor to yeast cytochrome c oxidase subunit IV (an imported mitochondrial protein) was attached to the amino-terminus of mouse dihydrofolate reductase (a cytosolic protein) by gene fusion. The resulting fusion protein was imported into the matrix of isolated, energized yeast mitochond...

journal_title：FEBS letters

authors： Hurt EC,Pesold-Hurt B,Schatz G

更新日期：1984-12-10 00:00:00

abstract：:In bacterial polypeptide synthesis aminoacyl-tRNA (aa-tRNA) bound to elongation factor Tu (EF-Tu) and GTP is part of a crucial intermediate ribonucleoprotein complex involved in the decoding of messenger RNA. The conformation and topology as well as the affinity of the macromolecules in this ternary aa-tRNA X EF-Tu X ...

journal_title：FEBS letters

authors： Faulhammer HG,Joshi RL

更新日期：1987-06-15 00:00:00

abstract：:A strain of yeast, Hansenula mrakii, secretes a toxin that kills sensitive yeasts, such as Saccharomyces cerevisiae Monoclonal antibodies raised against the toxin had both binding and neutralizing activities. The toxin in culture media was isolated by an affinity column of monoclonal antibody. The toxin is a basic pol...

journal_title：FEBS letters

authors： Yamamoto T,Imai M,Tachibana K,Mayumi M

更新日期：1986-01-20 00:00:00

abstract：:Preformed phosphatidylethanolamine-containing liposomes were thiolated with 2-iminothiolane (Traut's reagent) and subsequently activated by mixed disulfide formation with 5,5'-dithiobis(2-nitrobenzoic acid). Up to 65% of amino groups of the outer liposomal lamella, corresponding to 230 SH-groups per vesicle, were modi...

journal_title：FEBS letters

authors： Lasch J,Niedermann G,Bogdanov AA,Torchilin VP

更新日期：1987-04-06 00:00:00

abstract：:The Na+-induced release of accumulated Ca2+ from heart mitochondria is inhibited by amiloride, benzamil and several other amiloride analogues. These drugs do not affect uptake or release of Ca2+ mediated by the ruthenium red-sensitive uniporter and their effects, like those of diltiazem and other Ca2+-antagonists, app...

journal_title：FEBS letters

authors： Jurkowitz MS,Altschuld RA,Brierley GP,Cragoe EJ Jr

更新日期：1983-10-17 00:00:00

abstract：:Rho-associated kinase 2 (Rock2) is known to promote tumorigenesis in hepatocellular carcinoma (HCC). CCAAT/enhancer-binding protein delta (CEBPD) functions as a tumor suppressor. In this study, we found that the expression of Rock2 and CEBPD are inversely correlated. Knockdown of Rock2 increased CEBPD expression and i...

journal_title：FEBS letters

authors： Li M,Zhou W,Yuan R,Chen L,Liu T,Huang D,Hao L,Xie Y,Shao J

更新日期：2015-04-13 00:00:00

abstract：:The rate at which dinucleoside phosphates are cleaved by RNases is supposed to be determined by the mole fraction of enzyme-substrate complexes in which the phosphodiester moiety of a dinucleoside phosphate has a highly reactive conformation. The mole fraction of such complexes for a particular RNase depends on the na...

journal_title：FEBS letters

authors： Yakovlev GI,Bocharov AL,Moiseyev GP,Mikhaylov SN

更新日期：1985-01-07 00:00:00

abstract：:It was recently shown that there is a predominance of phase 1 introns near the cleavage site of signal peptides encoded by human genes. It was suggested that this biased distribution was due to intron insertion at AGmid R:G proto-splice sites. However, we found that there is no disproportional excess of AGmid R:G that...

journal_title：FEBS letters

authors： Vibranovski MD,Sakabe NJ,de Souza SJ

更新日期：2006-03-06 00:00:00

abstract：:A hybrid protein from Neisseria meningitidis, which contains both a peroxiredoxin and a glutaredoxin domain, has been isolated. The enzyme was active in the reduction of various peroxides and dehydroascorbate in the presence of reduced glutathione. These findings suggest that both the peroxiredoxin and glutaredoxin do...

journal_title：FEBS letters

authors： Rouhier N,Jacquot JP

更新日期：2003-11-06 00:00:00

abstract：:F-box/LRR-repeat protein 2 (FBXL2), a component of Skp-Cullin-F box (SCF) ubiquitin E3 ligase, has been shown to inhibit tumorigenesis by targeting and ubiquitinating several oncoproteins. However, its role in gastric cancer remains poorly understood. Here, by tandem mass spectrometry, we show that FBXL2 interacts wit...

journal_title：FEBS letters

authors： Li LQ,Pan D,Chen H,Zhang L,Xie WJ

更新日期：2016-02-01 00:00:00

abstract：:The degradation of type IX collagen, a minor collagen in cartilage, was examined by treatment with three different types of matrix metalloproteinases (MMPs) purified from the culture medium of rheumatoid synovial cells. Neither MMP-1 (collagenase) nor MMP-2 (so-called 'gelatinase') could digest type IX collagen, but M...

journal_title：FEBS letters

authors： Okada Y,Konomi H,Yada T,Kimata K,Nagase H

更新日期：1989-02-27 00:00:00

abstract：:We previously reported that tau protein kinase II (TPKII) from bovine brain was composed of 30 kDa and 23 kDa subunits. The 30 kDa subunit of TPKII can be regarded as a catalytic subunit because of its ATP-binding activity. Antibodies directed against TPKII-phosphorylated tau also reacted with tau phosphorylated by cd...

journal_title：FEBS letters

authors： Kobayashi S,Ishiguro K,Omori A,Takamatsu M,Arioka M,Imahori K,Uchida T

更新日期：1993-12-06 00:00:00

abstract：:Stimulation of platelets results in the liberation of arachidonic acid (AA) which is further metabolized via the cyclooxygenase or lipoxygenase (LPG) pathway. We have examined the effect of inhibition of LPG on (i) the ADP-induced increase of cytoplasmic Ca2+ concentration and (ii) platelet aggregation. Lipoxygenase i...

journal_title：FEBS letters

authors： Borin ML,Pinelis VG,Ivanova MA,Kudinov YuV,Azizova OA,Markov CM,Khodorov BI

更新日期：1989-11-06 00:00:00

abstract：:The unassigned open reading frame YDL142c was identified to code for cardiolipin synthase, Cls1p. A cls1 deletion strain is viable on glucose, galactose, ethanol, glycerol and lactate containing media, although the growth rate on non-fermentable carbon sources is decreased. Mitochondria of the cls1 mutant are devoid o...

journal_title：FEBS letters

authors： Tuller G,Hrastnik C,Achleitner G,Schiefthaler U,Klein F,Daum G

更新日期：1998-01-02 00:00:00

abstract：:PTPRO is often silenced by DNA hypermethylation in primary human tumors and cancer cell lines and functions as a tumor suppressor. Here we show that PTPRO is a target of E2F1. In addition, the microRNA cluster miR-17-92, another target of E2F1, participates in PTPRO regulation. PTPRO mRNA was up-regulated during S pha...

journal_title：FEBS letters

authors： Xu X,Hong Y,Kong C,Xu L,Tan J,Liang Q,Huang B,Lu J

更新日期：2008-08-20 00:00:00

abstract：:Transfer of naked plasmid with insulin precursor DNA into skeletal muscle of streptozotocin (STZ)-induced diabetic mice through electroporation and detection of gene expression is described. Four different human insulin precursor DNA fragments were inserted into pcDNA3.1(-), downstream of a CMV promoter. Three of them...

journal_title：FEBS letters

authors： Yin D,Tang JG

更新日期：2001-04-20 00:00:00

abstract：:The mechanisms that underlie axon formation are still poorly understood. GSK3 has been recently implicated in establishing the axon and in its elongation. We have used four different GSK3 inhibitors to determine the role of GSK3 activity in hippocampal neurons at different periods of time. Inhibition of GSK3 activity ...

journal_title：FEBS letters

authors： Garrido JJ,Simón D,Varea O,Wandosell F

更新日期：2007-04-17 00:00:00

abstract：:Recombinant porcine growth hormone (rPGH) was solubilised from inclusion bodies (IB's) using either 6 M guanidinium hydrochloride (GnHCl), 7.5 M urea or by a novel method using a cationic surfactant, cetyltrimethylammonium chloride (CTAC). Circular dichroism (CD) analysis of the secondary (2 degrees) structure of the ...

journal_title：FEBS letters

authors： Puri NK,Cardamone M

更新日期：1992-07-06 00:00:00

abstract：:The yeast PCK1 gene coding for phosphoenolpyruvate carboxykinase (PEPCK) was isolated by functional complementation of pck1 strains from S. cerevisiae. Only one copy of the gene was found per haploid yeast genome. An RNA of about 2 kb which hybridized with a DNA probe internal to the PCK1 gene was found only in cells ...

journal_title：FEBS letters

authors： Valdés-Hevia MD,de la Guerra R,Gancedo C

更新日期：1989-12-04 00:00:00