Abstract:
:Preformed phosphatidylethanolamine-containing liposomes were thiolated with 2-iminothiolane (Traut's reagent) and subsequently activated by mixed disulfide formation with 5,5'-dithiobis(2-nitrobenzoic acid). Up to 65% of amino groups of the outer liposomal lamella, corresponding to 230 SH-groups per vesicle, were modified. Covalent attachment of thiolated alpha-chymotrypsin to these thiol-liposomes via S-S bridges yielded a protein/lipid ratio of 3.6 X 10(-4) mol enzyme/mol lipid.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lasch J,Niedermann G,Bogdanov AA,Torchilin VPdoi
10.1016/0014-5793(87)80004-2subject
Has Abstractpub_date
1987-04-06 00:00:00pages
13-6issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(87)80004-2journal_volume
214pub_type
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