An interface point-mutation variant of triosephosphate isomerase is compactly folded and monomeric at low protein concentrations.

Abstract:

:Wild-type trypanosomal triosephosphate isomerase (wtTIM) is a very tight dimer. The interface residue His-47 of wtTIM has been mutated into an asparagine. Ultracentrifugation data show that this variant (H47N) only dimerises at protein concentrations above 3 mg/ml. H47N has been characterised at a protein concentration where it is predominantly a monomer. Circular dichroism measurements in the near-UV and far-UV show that this monomer is a compactly folded protein with secondary structure similar as in wtTIM. The thermal stability of the monomeric H47N is decreased compared to wtTIM: temperature gradient gel electrophoresis (TGGE) measurements give Tm-values of 41 degrees C for wtTIM, whereas the Tm-value for the monomeric form of H47N is approximately 7 degrees C lower.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Borchert TV,Zeelen JP,Schliebs W,Callens M,Minke W,Jaenicke R,Wierenga RK

doi

10.1016/0014-5793(95)00586-x

subject

Has Abstract

pub_date

1995-07-03 00:00:00

pages

315-8

issue

3

eissn

0014-5793

issn

1873-3468

pii

0014-5793(95)00586-X

journal_volume

367

pub_type

杂志文章
  • Efficient delivery of siRNA into cytokine-stimulated insulinoma cells silences Fas expression and inhibits Fas-mediated apoptosis.

    abstract::Fas/FasL interactions have been proposed as a potentially important mechanism mediating beta-cell death in type 1 diabetes. Recent investigations suggest RNA interference, afforded by small interfering RNAs (siRNA), can provide specific and robust gene silencing in mammalian cells. The current study attempted to inves...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2005.12.068

    authors: Burkhardt BR,Lyle R,Qian K,Arnold AS,Cheng H,Atkinson MA,Zhang YC

    更新日期:2006-01-23 00:00:00

  • Effects of substitutions of glycine and asparagine for serine132 on activity and binding of human lipoprotein lipase to very low density lipoproteins.

    abstract::For studying the role of Ser132 in the putative catalytic site of human lipoprotein lipase (LPL), mutant LPL cDNAs expressing LPLs with amino acid substitutions of Gly or Asn for Ser132 were obtained by site-directed mutagenesis, and were expressed in COS-1 cells. Considerable amounts of LPL enzyme protein mass were d...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(92)80017-b

    authors: Tashiro J,Kobayashi J,Shirai K,Saito Y,Fukamachi I,Hashimoto H,Nishida T,Shibui T,Morimoto Y,Yoshida S

    更新日期:1992-02-17 00:00:00

  • Islet amyloid: from fundamental biophysics to mechanisms of cytotoxicity.

    abstract::Pancreatic islet amyloid is a characteristic feature of type 2 diabetes. The major protein component of islet amyloid is the polypeptide hormone known as islet amyloid polypeptide (IAPP, or amylin). IAPP is stored with insulin in the β-cell secretory granules and is released in response to the stimuli that lead to ins...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.febslet.2013.01.046

    authors: Cao P,Marek P,Noor H,Patsalo V,Tu LH,Wang H,Abedini A,Raleigh DP

    更新日期:2013-04-17 00:00:00

  • Low-temperature microRNA expression in the painted turtle, Chrysemys picta during freezing stress.

    abstract::Natural freeze tolerance depends on cellular adaptations that address the multiple stresses imposed on cells during freezing. These adaptations preserve viability by suppressing energy-expensive cell processes in the frozen state. In this study, we explore the freeze-responsive expression of microRNA in hatchling pain...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2015.10.026

    authors: Biggar KK,Storey KB

    更新日期:2015-11-30 00:00:00

  • Specific inhibition of human beta-D-glucuronidase and alpha-L-iduronidase by a trihydroxy pipecolic acid of plant origin.

    abstract::The glucuronic acid analogue of 1-deoxynojirimycin, 2(S)-carboxy-3(R), 4(R), 5(S)-trihydroxypiperidine, recently isolated from seeds of Baphia racemosa, is a novel specific inhibitor of human liver beta-D-glucuronidase and alpha-L-iduronidase. No other glycosidases are inhibited. The inhibition of beta-D-glucuronidase...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(84)80911-4

    authors: Cenci di Bello I,Dorling P,Fellows L,Winchester B

    更新日期:1984-10-15 00:00:00

  • Evidence for co-regulation of Cu,Zn superoxide dismutase and metallothionein gene expression in yeast through transcriptional control by copper via the ACE 1 factor.

    abstract::Saccharomyces cerevisiae mutant strain DTY26, lacking ACE1, the protein mediator for the induction of metallothionein gene expression, is unable to increase Cu,Zn superoxide dismutase mRNA in response to copper. In the wild-type strain DTY22 transcription of both Cu,Zn superoxide dismutase and metallothionein genes is...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80131-l

    authors: Carri MT,Galiazzo F,Ciriolo MR,Rotilio G

    更新日期:1991-01-28 00:00:00

  • Modulation of cardiac Ca2+ channels in Xenopus oocytes by protein kinase C.

    abstract::L-Type calcium channel was expressed in Xenopus laevis oocytes injected with RNAs coding for different cardiac Ca2+ channel subunits, or with total heart RNA. The effects of activation of protein kinase C (PKC) by the phorbol ester PMA (4 beta-phorbol 12-myristate 13-acetate) were studied. Currents through channels co...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(92)80980-u

    authors: Singer-Lahat D,Gershon E,Lotan I,Hullin R,Biel M,Flockerzi V,Hofmann F,Dascal N

    更新日期:1992-07-20 00:00:00

  • Delta(9)-tetrahydrocannabinol regulates the p53 post-translational modifiers Murine double minute 2 and the Small Ubiquitin MOdifier protein in the rat brain.

    abstract::The phytocannabinoid Delta(9)-Tetrahydrocannabinol (Delta(9)-THC), the main psychoactive cannabinoid in cannabis, activates a number of signalling cascades including p53. This study examines the role of Delta(9)-THC in regulating the p53 post-translational modifier proteins, Murine double minute (Mdm2) and Small Ubqui...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2009.09.056

    authors: Gowran A,Murphy CE,Campbell VA

    更新日期:2009-11-03 00:00:00

  • Secretion of 5-hydroxytryptamine from electropermeabilised human platelets. Effects of GTP and cyclic 3',5'-AMP.

    abstract::Enhancement by thrombin of Ca2+-dependent 5HT secretion in the absence of added GTP decreases as the time between electropermeabilisation and addition of thrombin is increased. No decrease occurs if thrombin is added with GTP. Observation of apparent GTP-independent receptor/phospholipase C coupling may result from th...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(87)80507-0

    authors: Knight DE,Scrutton MC

    更新日期:1987-10-19 00:00:00

  • ADP-ribosylation of nuclear proteins is increased by phenobarbital. Identification of the ADP-ribosylated histone fractions in rat liver nuclei.

    abstract::Changes in the ADP-ribosylation of total proteins and purified histones of rat liver nuclei after phenobarbital treatment (80 mg/kg, 24 h) have been studied. The [32P]NAD incorporation into total trichloroacetic acid precipitated proteins, in histone Hl and in core histones was evaluated, the specific radioactivities ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(86)80472-0

    authors: Bràz J,Lechner MC

    更新日期:1986-04-21 00:00:00

  • Bile salt activation of human cholesterol esterase does not require protein dimerisation.

    abstract::Human milk cholesterol esterase (bile salt-activated lipase) plays a role in the dietary uptake of triacylglyceride and cholesteryl ester. The activities toward these substrates are mediated through a unique bile salt-activated mechanism. Previously, it has been proposed that a necessary step in this process is prior ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(97)00215-9

    authors: Loomes KM,Senior HE

    更新日期:1997-04-01 00:00:00

  • The polytopic mitochondrial inner membrane proteins MIM17 and MIM23 operate at the same preprotein import site.

    abstract::Three proteins of the mitochondrial inner membrane are known that are essential for the viability of yeast and seem to be involved in import of preproteins; the integral membrane proteins MIM17 and MIM23 and the peripheral membrane protein MIM44, MIM17 and MIM23 are homologous to each other in their hydrophobic domain...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(94)00670-9

    authors: Kübrich M,Keil P,Rassow J,Dekker PJ,Blom J,Meijer M,Pfanner N

    更新日期:1994-08-01 00:00:00

  • Insight into conformational modification of alpha-synuclein in the presence of neuronal whole cells and of their isolated membranes.

    abstract::A change in the conformational plasticity of α-Synuclein (α-Syn) is hypothesised to be a key step in the pathogenic mechanism of Parkinson's disease (PD). Here, we report the study of extracellular α-Syn interaction with whole cells and membranes isolated from the neuronal SH-SY5Y cells, exploiting NMR and CD spectros...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2015.02.012

    authors: Smaldone G,Diana D,Pollegioni L,Di Gaetano S,Fattorusso R,Pedone E

    更新日期:2015-03-24 00:00:00

  • CITRX thioredoxin is a putative adaptor protein connecting Cf-9 and the ACIK1 protein kinase during the Cf-9/Avr9- induced defence response.

    abstract::Tomato Cf-9, a receptor-like protein (RLP), confers resistance to races of the fungal pathogen Cladosporium fulvum that express the Avr9 avirulence gene. CITRX (Cf-9-interacting thioredoxin) was previously identified in a yeast two-hybrid screen as a protein interacting with the cytoplasmic domain of Cf-9 and shown to...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2006.06.077

    authors: Nekrasov V,Ludwig AA,Jones JD

    更新日期:2006-07-24 00:00:00

  • Impact of 9-(2-phosphonylmethoxyethyl)adenine on (deoxy)ribonucleotide metabolism and nucleic acid synthesis in tumor cells.

    abstract::Following exposure to 9-(2-phosphonylmethoxyethyl)adenine (an inhibitor of the cellular DNA polymerases alpha, delta and epsilon), human erythroleukemia K562, human T-lymphoid CEM and murine leukemia L1210 cells markedly accumulated in the S phase of the cell cycle. In contrast to DNA replication, RNA synthesis (trans...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(99)00104-0

    authors: Hatse S,De Clercq E,Balzarini J

    更新日期:1999-02-19 00:00:00

  • The origin of the sodium-dependent NADH oxidation by the respiratory chain of Klebsiella pneumoniae.

    abstract::Properties of Klebsiella pneumoniae respiratory chain enzymes catalyzing NADH oxidation have been studied. Using constructed K. pneumoniae mutant strains, it was shown that three enzymes belonging to different families of NADH:quinone oxidoreductases operate in this bacterium. The NDH-2-type enzyme is not coupled with...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/S0014-5793(04)00312-6

    authors: Bertsova YV,Bogachev AV

    更新日期:2004-04-09 00:00:00

  • Reversible unfolding of dimeric phosphofructokinase-2 from Escherichia coli reveals a dominant role of inter-subunit contacts for stability.

    abstract::Escherichia coli phosphofructokinase-2 (Pfk-2) is a homodimer whose subunits consist of a large domain and an additional beta-sheet that provides the interfacial contacts between the subunits, creating a beta-barrel flattened-like structure with the adjacent subunit's beta-sheet. To determine how the structural organi...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2009.05.034

    authors: Baez M,Babul J

    更新日期:2009-06-18 00:00:00

  • ATP synthesis catalyzed by the mitochondrial F1-F0 ATP synthase is not a reversal of its ATPase activity.

    abstract::The ADP(Mg2+)-deactivated oligomycin-sensitive F1-F0 ATPase of coupled submitochondrial particles treated with the substoichiometric amount of oligomycin was studied to test whether ATP synthesis and hydrolysis proceed in either direction through the same intermediates. The initial rates of ATP hydrolysis, oxidative p...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(95)00487-t

    authors: Syroeshkin AV,Vasilyeva EA,Vinogradov AD

    更新日期:1995-06-05 00:00:00

  • Effects of 'hydrophobic mismatch' on the location of transmembrane helices in the ER membrane.

    abstract::We have studied the effects of 'hydrophobic mismatch' between a poly-Leu transmembrane helix (TMH) and the ER membrane using a glycosylation mapping approach. The simplest interpretation of our results is that the lumenal end of the TMH is located deeper in the membrane for both short (negative mismatch) and long (pos...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(01)02415-2

    authors: Monné M,von Heijne G

    更新日期:2001-05-11 00:00:00

  • Dictyostelium discoideum requires an Alix/AIP1 homolog, DdAlix, for morphogenesis in alkaline environments.

    abstract::Alix and its homologs are involved in various phenomena such as endosomal protein-sorting and adaptation to stress conditions. In this study, we found that development of Dictyostelium discoideum Alix (DdAlix) deletion mutant (alx-) cells was impaired in alkaline pH environments. The fruiting body formation efficiency...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2005.02.036

    authors: Ohkouchi S,Saito H,Aruga F,Maeda T,Shibata H,Maki M

    更新日期:2005-03-14 00:00:00

  • Hydrolyse des aminoacyl-tARN par la phosphodiesterase du venin.

    abstract::The influence of the esterification of the 3'(2') hydroxyl group of tRNA on the hydrolysis by venom phosphodiesterase (Crotalus adamanteus) was studied. It was shown that this esterification does not change the rate of hydrolysis. At pH 7-8, no significant differences were observed either when the alpha amino group of...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(68)80046-8

    authors: Yot P,Gueguen P,Chapeville F

    更新日期:1968-08-01 00:00:00

  • Non-radioactive reverse transcriptase/polymerase chain reaction for quantification of myosin heavy chain mRNA isoforms in various rabbit muscles.

    abstract::A method was established for measuring molecule numbers of three different myosin heavy chain (MHC) mRNA isoforms in total RNA preparations. The quantification was based on a combination of primer-directed reverse transcriptase and polymerase chain reactions with 5'-digoxigenin-labeled oligonucleotides, using external...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)80523-w

    authors: Peuker H,Pette D

    更新日期:1993-03-08 00:00:00

  • Adrenal chromaffin granules and secretory granules from thyroid parafollicular cells have several common antigens.

    abstract::The presence of various antigens in two types of isolated endocrine vesicles (chromaffin granules and secretory vesicles of thyroid parafollicular cells) was investigated by immunoblotting. The two types of vesicles have three common secretory proteins: chromogranin A, chromogranin B and secretogranin II. Furthermore,...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(89)81595-9

    authors: Weiler R,Cidon S,Gershon MD,Tamir H,Hogue-Angeletti R,Winkler H

    更新日期:1989-11-06 00:00:00

  • Conserved core tryptophans of FnII domains are crucial for the membranolytic and chaperone-like activities of bovine seminal plasma protein PDC-109.

    abstract::The fibronectin type II (FnII) domain, present in diverse vertebrate proteins, plays crucial roles in several fundamental biological processes. PDC-109, the major bovine seminal plasma protein, contains two FnII domains that bind to choline phospholipids on sperm plasma membrane and induce lipid efflux crucial for suc...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1002/1873-3468.13617

    authors: Singh BP,Asthana A,Basu A,Tangirala R,Mohan Rao C,Swamy MJ

    更新日期:2020-02-01 00:00:00

  • Recognition of an antiparallel beta-sheet structure of human epidermal growth factor by its receptor. Site-directed mutagenesis studies of Ala-30 and Asn-32.

    abstract::The Ala-30 and Asn-32 residues involved in the major antiparallel beta-sheet structure of human epidermal growth factor (hEGF) were substituted with various amino acid residues, and the receptor-binding affinities of the nine variant hEGFs were determined by the use of human KB cells. The Ala-30----Arg, Ala-30----His ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(92)80279-p

    authors: Koide H,Muto Y,Kasai H,Hoshi K,Takusari H,Kohri K,Takahashi S,Sasaki T,Tsukumo K,Miyake T

    更新日期:1992-05-04 00:00:00

  • Presence of the gypsy (MDG4) retrotransposon in extracellular virus-like particles.

    abstract::As a first step to investigate the functional activity of gypsy virus-like particles (VLPs) we explored the possibility of preservation of its VLP in extracellular form. The preparations containing extracellular gypsy VLP from Drosophila melanogaster and D. virilis were obtained. Full-length polyA+ RNA and polyA+ RNA-...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)81358-7

    authors: Syomin BV,Kandror KV,Semakin AB,Tsuprun VL,Stepanov AS

    更新日期:1993-06-01 00:00:00

  • A 500 MHz study of peptide T in a DMSO solution.

    abstract::Peptide T, an octapeptide of sequence ASTTTNYT that binds to human T cells, was studied as a zwitterion in DMSOd6 solution by means of proton NMR spectroscopy at 500 MHz. The unusual dispersion of the resonances of residues of the same type (T) makes it possible to assign all resonances to specific residues by means o...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(88)80723-3

    authors: Picone D,Temussi PA,Marastoni M,Tomatis R,Motta A

    更新日期:1988-04-11 00:00:00

  • The role of oxidative stress in Friedreich's ataxia.

    abstract::Oxidative stress and an increase in the levels of free radicals are important markers associated with several pathologies, including Alzheimer's disease, cancer and diabetes. Friedreich's ataxia (FRDA) is an excellent paradigmatic example of a disease in which oxidative stress plays an important, albeit incompletely u...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1002/1873-3468.12928

    authors: Lupoli F,Vannocci T,Longo G,Niccolai N,Pastore A

    更新日期:2018-03-01 00:00:00

  • Effects of analogues of adenosine and methyl xanthines on insulin sensitivity in soleus muscle of the rat.

    abstract::The concentration of insulin that produces half-maximal stimulation of glycolysis by stripped soleus muscle preparations is markedly increased by the adenosine analogues, 2-chloroadenosine and N6-phenylisopropyladenosine, but is markedly decreased by the methyl xanthine analogue, 8-phenyltheophylline. 2-Chloroadenosin...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(84)80820-0

    authors: Budohoski L,Challiss RA,McManus B,Newsholme EA

    更新日期:1984-02-13 00:00:00

  • Ankrd17, an ubiquitously expressed ankyrin factor, is essential for the vascular integrity during embryogenesis.

    abstract::Ankyrin repeat domain 17 (Ankrd17) encodes an ubiquitously expressed protein with two clusters of ankyrin repeats. We have used gene targeting strategy to ablate the Ankrd17 gene in mouse. The Ankrd17-deficient mice died between embryonic day (E) 10.5 and E11.5 due to cardiovascular defects. Serious hemorrhages were d...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2009.07.025

    authors: Hou SC,Chan LW,Chou YC,Su CY,Chen X,Shih YL,Tsai PC,Shen CK,Yan YT

    更新日期:2009-09-03 00:00:00