Enhancement and inhibition of snake venom phosphodiesterase activity by lysophospholipids.

abstract：:The accumulation of protoporphyrin IX (Proto IX) in light-sensitive mutants of Escherichia coli was detected by spectrofluorimetry. Fluorescence emission and excitation spectra were recorded from extracts of bacterial cells. Proto IX clearly accumulated in cells with mutations in the visA (hemH) gene but not in the wi...

journal_title：FEBS letters

authors： Miyamoto K,Nishimura K,Masuda T,Tsuji H,Inokuchi H

更新日期：1992-10-05 00:00:00

abstract：:Plasma citrate levels were found to be elevated in non-alcoholic fatty liver disease (NAFLD) patients. Cellular experiments indicated that increased citrate levels might originate from an excess of fatty acids. The impact of elevated citrate levels on oxidative stress was examined. It was found that citrate stimulated...

journal_title：FEBS letters

authors： van de Wier B,Balk JM,Haenen GR,Giamouridis D,Bakker JA,Bast BC,den Hartog GJ,Koek GH,Bast A

更新日期：2013-08-02 00:00:00

abstract：:Oscillations of free intracellular calcium [Ca2+]i were seen in individual Jurkat cells as response to a 50 Hz, 0.15 mT magnetic field (MF). In contrast, a CD45-deficient Jurkat cell line was unable to respond to MF stimulation. The phosphatase activity of CD45 has been implicated to regulate p56lck tyrosine kinase ac...

journal_title：FEBS letters

authors： Lindström E,Berglund A,Mild KH,Lindström P,Lundgren E

更新日期：1995-08-14 00:00:00

abstract：:Novel catechin derivatives obtained from grape procyanidins and l-cysteine scavenge free radicals by hydrogen atom donation, rather than electron transfer, and reduce cell viability in A375 and M21 melanoma cells. In particular, 4beta-(S-cysteinyl)epicatechin 3-O-gallate has a free radical scavenging capacity as stron...

journal_title：FEBS letters

authors： Lozano C,Torres JL,Julià L,Jimenez A,Centelles JJ,Cascante M

更新日期：2005-08-15 00:00:00

abstract：:We show that anthocyanins can function as potent inhibitors of the formation of nitrated tyrosine in vitro, and clarified how pelargonidin (Pel), which has a mono-hydroxyl group on the B-ring, can scavenge peroxynitrite (ONOO(-)) by detection of the reaction products. Pel was reacted with ONOO(-), then the reaction mi...

journal_title：FEBS letters

authors： Tsuda T,Kato Y,Osawa T

更新日期：2000-11-10 00:00:00

abstract：:Apoptosis specific proteins (ASP) are expressed in the cytoplasm of cultured mammalian cells of various lineages following induction of apoptosis. The cDNA encoding ASP has been cloned from a human expression library and has significant homology to the Saccharomyces cerevisiae APG5 gene which is essential for yeast au...

journal_title：FEBS letters

authors： Hammond EM,Brunet CL,Johnson GD,Parkhill J,Milner AE,Brady G,Gregory CD,Grand RJ

更新日期：1998-04-03 00:00:00

abstract：:Selective protein degradation is an energy-dependent process performed by high-molecular-weight proteases. The activity of proteolytic components of these enzymes is coupled to the ATPase activity of their regulatory subunits or domains. Here, we obtained the proteolytic domain of Escherichia coli protease Lon by clon...

journal_title：FEBS letters

authors： Rasulova FS,Dergousova NI,Starkova NN,Melnikov EE,Rumsh LD,Ginodman LM,Rotanova TV

更新日期：1998-08-07 00:00:00

abstract：:The nucleotide sequence of the dnaK operon cloned from Porphyromonas gingivalis revealed that the operon does not contain homologues of either dnaJ or grpE. However, there were two genes which encode small heat shock proteins immediately downstream from the dnaK and they were transcribed together with dnaK as one unit...

journal_title：FEBS letters

authors： Yoshida A,Nakano Y,Yamashita Y,Oho T,Shibata Y,Ohishi M,Koga T

更新日期：1999-03-12 00:00:00

abstract：:The chemokine RANTES is a potent chemoattractant for eosinophils. RANTES is produced by lung epithelial cells during eosinophil-rich inflammatory diseases such as asthma. In this study, we examined the effects of thiazolidinediones (TZD) on RANTES expression in a human lung epithelial cell line, A549. In A549 cells, i...

journal_title：FEBS letters

authors： Momoi A,Murao K,Imachi H,Sayo Y,Nakamura H,Hosokawa H,Sato M,Fujita J,Okada H,Ishida T,Takahara J

更新日期：1999-06-11 00:00:00

abstract：:The Escherichia coli signal transduction protein PII, product of the glnB gene, was overproduced and purified. The predicted molecular weight of the protein based on the correct nucleotide sequence is 12,427 and is very close to the value 12,435 obtained by matrix-assisted laser desorption mass spectrometry. Hexagonal...

journal_title：FEBS letters

authors： Vasudevan SG,Gedye C,Dixon NE,Cheah E,Carr PD,Suffolk PM,Jeffrey PD,Ollis DL

更新日期：1994-01-17 00:00:00

abstract：:Lectin-like oxidized low-density lipoprotein receptor (LOX-1) is a scavenger receptor that binds oxidized low-density lipoprotein (OxLDL) and has a role in atherosclerosis development. The N-terminus intracellular region (cytoplasmic domain) of LOX-1 mediates receptor internalization and trafficking, potentially throu...

journal_title：FEBS letters

authors： Bakthavatsalam D,Soung RH,Tweardy DJ,Chiu W,Dixon RA,Woodside DG

更新日期：2014-06-13 00:00:00

abstract：:Leishmania major promastigotes are parasites endowed with a plasma membrane electrogenic H+ pump and anionic channels. These systems have been thought to contribute to pH homeostasis of parasites and environmental adaptation by mediating extrusion of protons which are either generated metabolically or result from exog...

journal_title：FEBS letters

authors： Vieira L,Cabantchik ZI

更新日期：1995-03-13 00:00:00

abstract：:In the first committed reaction of chlorophyll biosynthesis, magnesium chelatase couples ATP hydrolysis to the thermodynamically unfavorable Mg(2+) insertion into protoporphyrin IX (ΔG°' of circa 25-33 kJ·mol(-1) ). We explored the thermodynamic constraints on magnesium chelatase and demonstrate the effect of nucleoti...

journal_title：FEBS letters

authors： Adams NB,Brindley AA,Hunter CN,Reid JD

更新日期：2016-06-01 00:00:00

abstract：:We have investigated the functional importance of nucleotide sequence differences between proximal (P-) and distal (D-) p53-binding elements in the MCK promoter. P- and D-elements normally co-operate to permit synergistic promoter activation by p53. Interestingly, we find that P-elements cannot co-operate with each ot...

journal_title：FEBS letters

authors： Jackson P,Yardley G

更新日期：1997-04-14 00:00:00

abstract：:Previous electrophysiological experiments characterized the Sec61 complex, which provides the aqueous path for entry of newly-synthesized polypeptides into the mammalian endoplasmic reticulum, as a highly dynamic channel that, once activated by precursor proteins, fluctuates between main open states with mean conducta...

journal_title：FEBS letters

authors： Erdmann F,Jung M,Eyrisch S,Lang S,Helms V,Wagner R,Zimmermann R

更新日期：2009-07-21 00:00:00

abstract：:The failure of insulin-regulated recruitment of the GLUT4 glucose transporter in cardiac muscle of obese Zucker rats is associated with alterations of the subcellular distribution of the small-molecular-mass GTP-binding protein rab4A. Here, we show by subcellular fractionation and Western blotting a translocation of t...

journal_title：FEBS letters

authors： Uphues I,Chern Y,Eckel J

更新日期：1995-12-18 00:00:00

abstract：:A variant form of the heptacosapeptide amide secretin, with C-terminal -Val-Gly-Lys-Arg instead of valine amide, has been isolated from porcine upper intestinal tissue. Unexpectedly, this triacontapeptide exhibited a substantially higher bioactivity than the heptacosapeptide amide. ...

journal_title：FEBS letters

authors： Gafvelin G,Carlquist M,Mutt V

更新日期：1985-05-20 00:00:00

abstract：:5-(1-Azidovinyl)-2'-deoxyuridine (AzVDU) and a series of 5-[1-azido-2-halogenoethyl]-derivatives of beta-D-arabinofuranosyluracil (AU) proved markedly inhibitory to the replication of herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV), but not thymidine kinase (TK)-deficient HSV-1 and VZV strains. No...

journal_title：FEBS letters

authors： Balzarini J,Andrei G,Kumar R,Knaus EE,Wiebe LI,De Clercq E

更新日期：1995-10-02 00:00:00

abstract：:The replacement of O2 with CO was studied on cobalt-iron hemoglobin hybrids. Both proto- and mesocobalt hemes were used for the reconstitution. In the oxy quaternary conformation no difference is observed between alpha- and beta-subunits when only proto hemes are present in the hybrid (k4 = 30 s-1, k'4/l'4 = 2.5). If ...

journal_title：FEBS letters

authors： Oertle M,Winterhalter KH,Di Iorio EE

更新日期：1983-03-07 00:00:00

abstract：:L- and D-specific nicotinamide adenine dinucleotide (NAD)-dependent dehydrogenases map to the same structural protein superfamily as defined by the Structural Classification of Proteins (SCOP) and are based on the Rossmann fold type domains. A detailed classification of these domains is proposed using a novel diagnost...

journal_title：FEBS letters

authors： Kutzenko AS,Lamzin VS,Popov VO

更新日期：1998-02-13 00:00:00

abstract：:Cell migration over or through the extracellular matrix (ECM) is an integral feature of both physiological and pathological processes. Regulation of the changing cell-ECM interactions involved can be effected by proteolysis and requires strict spatial and temporal targeting of proteinase activity. The versatile use of...

journal_title：FEBS letters

authors： Ellis V,Murphy G

更新日期：2001-09-28 00:00:00

abstract：:The tropomyosin-related kinase A (TrkA) receptor and its ligand, nerve growth factor (NGF), play crucial roles in the development and function of the nervous system. NGF is believed to activate TrkA by bridging two TrkA monomers, leading to TrkA transphosphorylation. However, here we show that the majority of TrkA rec...

journal_title：FEBS letters

authors： Shen J,Maruyama IN

更新日期：2011-01-21 00:00:00

abstract：:Deuterated arachidonic acid (AA, [2H8]20:4 n-6) 1-25 microM, is converted to other fatty acids, as evaluated by gas chromatography-mass spectrometry, in THP-1 cells. The major products, in the 1 to 10 microM range, are 22:4 (elongated) and 20:3 (reduced in 5). At 25 microM, 24:4, 24:5 and 22:5 accumulate, with [2H8]/[...

journal_title：FEBS letters

authors： Caruso D,Risé P,Galella G,Regazzoni C,Toia A,Galli G,Galli C

更新日期：1994-05-02 00:00:00

abstract：:In rat cerebrocortical synaptosomes, the addition of 4 beta-phorbol dibutyrate (4 beta-PDBu) and arachidonic acid enhances and decreases, respectively, the glutamate release evoked by 4-aminopyridine. Pretreatment of synaptosomes with 12-O-tetradecanoylphorbol 13-acetate (TPA) or pre-incubation with staurosporine, pre...

journal_title：FEBS letters

authors： Herrero I,Miras-Portugal MT,Sánchez-Prieto J

更新日期：1992-01-27 00:00:00

abstract：:Sensitivity of mammalian cells to the bacterial toxin aerolysin is due to the presence at their surface of glycosylphosphatidyl inositol (GPI)-anchored proteins which act as receptors. Using a panel of mutants that are affected in the GPI biosynthetic pathway and Trypanosoma brucei variant surface glycoproteins, we sh...

journal_title：FEBS letters

authors： Abrami L,Velluz MC,Hong Y,Ohishi K,Mehlert A,Ferguson M,Kinoshita T,Gisou van der Goot F

更新日期：2002-02-13 00:00:00

abstract：:The further biochemical characterization and subcellular localization of cardiotin, a high molecular weight (300 kDa) constituent of cardiac muscle, is described. Immunofluorescence assays revealed a colocalization of cardiotin and the Ca2+ pump SERCA2a in the longitudinal sarcoplasmic reticulum (SR). However, in cont...

journal_title：FEBS letters

authors： Schaart G,Moens L,Endert JM,Ramaekers FC

更新日期：1997-02-17 00:00:00

abstract：:Cytosolic sialidase Neu2 has been implicated in myoblast differentiation. Here we observed a significant upregulation of Neu2 expression during differentiation of murine C2C12 myoblasts. This was evidenced both as an increase in Neu2 mRNA steady-state levels and in the cytosolic sialidase enzymatic activity. To unders...

journal_title：FEBS letters

authors： Fanzani A,Giuliani R,Colombo F,Zizioli D,Presta M,Preti A,Marchesini S

更新日期：2003-07-17 00:00:00

abstract：:Understanding protein beta structures has been hindered by the challenge of designing small, well-folded β-sheet systems. A β-capping motif was previously designed to help solve this problem, but not without limitations, as the termini of this β-cap were not fully available for chain extension. Combining Coulombic sid...

journal_title：FEBS letters

authors： Anderson JM,Kier BL,Shcherbakov AA,Andersen NH

更新日期：2014-12-20 00:00:00

abstract：:Evidence has previously suggested that cellobiose:quinone oxidoreductase (CBQ) in cellulolytic cultures of Phanerochaete chrysosporium might be produced from cellobiose oxidase (CBO) by proteolytic cleavage. This study demonstrates that the ratio of CBO activity to (CBO + CBQ) activity declines with decreasing culture...

journal_title：FEBS letters

authors： Habu N,Samejima M,Dean JF,Eriksson KE

更新日期：1993-07-26 00:00:00

abstract：:Soluble aluminium (Al(3+)) is the major constraint to plant growth on acid soils. Plants have evolved mechanisms to tolerate Al(3+) and one type of mechanism relies on the efflux of organic anions that protect roots by chelating the Al(3+). Al(3+) resistance genes of several species have now been isolated and found to...

journal_title：FEBS letters

authors： Delhaize E,Gruber BD,Ryan PR

更新日期：2007-05-25 00:00:00