Cold denaturation of yeast phosphoglycerate kinase: which domain is more stable?

Abstract:

:Under destabilising conditions both heat and cold denaturation of yeast phosphoglycerate kinase (PGK) can be observed. According to previous interpretation of experimental data and theoretical calculations, the C-terminal domain should be more stable than the N-terminal domain at all temperatures. We report on thermal unfolding experiments with PGK and its isolated domains, which give rise to a revision of this view. While the C-terminal domain is indeed the more stable one on heating, it reveals lower stability in the cold. These findings are of importance, because PGK has been frequently used as a model for protein folding and mutual domain interactions.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Gast K,Damaschun G,Desmadril M,Minard P,Müller-Frohne M,Pfeil W,Zirwer D

doi

10.1016/0014-5793(94)01437-6

subject

Has Abstract

pub_date

1995-01-30 00:00:00

pages

247-50

issue

3

eissn

0014-5793

issn

1873-3468

pii

0014-5793(94)01437-6

journal_volume

358

pub_type

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