Blockade of ADP-induced Ca2+-signal and platelet aggregation by lipoxygenase inhibitors.

abstract：:Water-soluble amyloid beta-peptides (sA beta), ending at residue 42, precede amyloid plaques in Down's syndrome (DS). Here we report that sA beta consists of the full-length A beta(1-42) and peptides truncated and modified by cyclization of the N-terminal glutamates, A beta[3(pE)-42] and A beta[11(pE)-42]. The A beta[...

journal_title：FEBS letters

authors： Russo C,Saido TC,DeBusk LM,Tabaton M,Gambetti P,Teller JK

更新日期：1997-06-16 00:00:00

abstract：:In humans, many responses to hypoxia including angiogenesis and erythropoiesis are mediated by the alpha/beta-heterodimeric transcription factor hypoxia inducible factor (HIF). The stability and/or activity of human HIF-1alpha are modulated by post-translational modifications including prolyl and asparaginyl hydroxyla...

journal_title：FEBS letters

authors： Murray-Rust TA,Oldham NJ,Hewitson KS,Schofield CJ

更新日期：2006-04-03 00:00:00

abstract：:In a wild-type strain of Saccharomyces cerevisiae, cadmium induces the activities of both gamma-glutamyl transferase (gamma-GT) and glutathione transferase 2 (Gtt2). However, Gtt2 activity did not increase under gamma-GT or Ycf1 deficiencies, suggesting that the accumulation of glutathione-cadmium in the cytosol inhib...

journal_title：FEBS letters

authors： Adamis PD,Mannarino SC,Eleutherio EC

更新日期：2009-05-06 00:00:00

abstract：:Glycosaminoglycans (GAGs) are complexed with plasma proteins and proteolysis of plasma reduced the protein-GAG ratio about 140-fold. After dialysis, analysis by gradient PAGE revealed heparinase-1-sensitive GAGs, thus suggesting that heparin could be among the plasma GAGs. However, after dialysis most of the plasma GA...

journal_title：FEBS letters

authors： Chevanne M,Caldini R,Manao G,Ruggiero M,Vannucchi S

更新日期：1999-12-10 00:00:00

abstract：:The malaria parasite has for long been thought to escape host complement attack as a survival strategy. However, it was only recently that complement evasion mechanisms of the parasite were described. Simultaneously, the role of complement in antibody-mediated naturally acquired and vaccine-induced protection against ...

journal_title：FEBS letters

authors： Kiyuka PK,Meri S,Khattab A

更新日期：2020-08-01 00:00:00

abstract：:Mutual arrangement of histone H1 molecules in chromatin extended in low salt-EDTA buffer and additionally in the presence of urea was studied by means of reversible cross-linking combined with chymotryptic digestion. In the chromatins tested, the chymotryptic halves of H1 were cross-linked in all possible combinations...

journal_title：FEBS letters

authors： Nikolaev LG,Glotov BO,Dashkevich VK,Barbashov SF,Severin ES

更新日期：1983-10-31 00:00:00

abstract：:Nitrate compartmentalization in intracellular organelles has been long recognized as critical for plant physiology but the molecular identity of the proteins involved remained unclear for a long time. In Arabidopsis thaliana, AtClC-a has been recently shown to be a NO(3)(-)/H(+) antiporter critical for nitrate transpo...

journal_title：FEBS letters

authors： Zifarelli G,Pusch M

更新日期：2010-05-17 00:00:00

abstract：:Proteins and peptides are readily and specifically modified at their sulfhydryl groups by the vapors of a mixture of 4-vinylpyridine and tributylphosphine. The phenylthiohydantoin derivative of S-beta-(4-pyridylethyl)cysteine formed during sequence analysis is easily detectable in current identification systems. ...

journal_title：FEBS letters

authors： Amons R

更新日期：1987-02-09 00:00:00

abstract：:Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be dis...

journal_title：FEBS letters

authors： Laukens K,Lenobel R,Strnad M,Van Onckelen H,Witters E

更新日期：2003-01-02 00:00:00

abstract：:Ribosome display is a powerful tool for selecting and evolving protein functions through ligand-binding. Here, this in vitro system was used to perform selection based on the folding properties of proteins, independent of specific ligand-binding. The selection is based on two properties of misfolded proteins: (1) incr...

journal_title：FEBS letters

authors： Matsuura T,Plückthun A

更新日期：2003-03-27 00:00:00

abstract：:Hemin stimulates cAMP production in human peripheral blood mononuclear cells (PBMC). The kinetics are similar to that of hormone-induced cAMP generation, namely a rapid effect followed by a desensitization phase. Several experimental findings suggest that prostaglandins do not mediate this effect. First, macrophage de...

journal_title：FEBS letters

authors： Lander HM,Levine DM,Novogrodsky A

更新日期：1992-06-01 00:00:00

abstract：:Stroke is characterized by massive inflammation in areas surrounding the injury that magnifies damage to the brain. The liver X receptors (LXRs) are nuclear receptors that regulate cholesterol, lipid, and glucose metabolism. Synthetic LXR agonists have potent anti-inflammatory properties in a variety of settings, incl...

journal_title：FEBS letters

authors： Sironi L,Mitro N,Cimino M,Gelosa P,Guerrini U,Tremoli E,Saez E

更新日期：2008-10-15 00:00:00

abstract：:Fourteen truncated forms of Leuconostoc mesenteroides NRRL B512-F dextransucrase, involving N-, C- or N- plus C-terminal domain truncations were tested for their ability to bind dextrans. The shortest fragment (14kDa molecular weight) that still exhibited a strong interaction with dextran was localized between amino a...

journal_title：FEBS letters

authors： Suwannarangsee S,Moulis C,Potocki-Veronese G,Monsan P,Remaud-Simeon M,Chulalaksananukul W

更新日期：2007-10-02 00:00:00

abstract：:Herein, we report that dihydrolipoic acid and lipoic acid (LA) plus lipoamide dehydrogenase and NADH denitrosate S-nitrosocaspase 3 (CASP-SNO). In HepG2 cells, S-nitroso-L-cysteine ethyl ester (SNCEE) impeded the activity of caspase 3 (CASP-SH), while a subsequent incubation of the cells in SNCEE-free medium resulted ...

journal_title：FEBS letters

authors： Sengupta R,Billiar TR,Atkins JL,Kagan VE,Stoyanovsky DA

更新日期：2009-11-03 00:00:00

abstract：:The enzyme telomerase is necessary for the synthesis and maintenance of telomere length. The catalytic subunit, telomerase reverse transcriptase (TERT), is regulated by interaction with the 90 kDa heat shock protein (HSP90) and by Akt-dependent phosphorylation. Here, we demonstrate that HSP90 and Akt physically intera...

journal_title：FEBS letters

authors： Haendeler J,Hoffmann J,Rahman S,Zeiher AM,Dimmeler S

更新日期：2003-02-11 00:00:00

abstract：:Cloning and nucleotide sequence analysis of the human calcitonin mRNA from the BEN lung carcinoma cell line, a cell line known to secrete high-Mr forms of calcitonin, showed no difference in the coding region at the nucleotide level compared with calcitonin mRNA isolated from medullary thyroid carcinoma which secretes...

journal_title：FEBS letters

authors： Riley JH,Edbrooke MR,Craig RK

更新日期：1986-03-17 00:00:00

abstract：:The classical short-term effect (within minutes) of arginine vasopressin (AVP) consists in increasing sodium, chloride and water transport in kidney cells. More recently, long-term actions (several hours) of the hormone have been evidenced on water and sodium fluxes, due to transcriptional enhancement in the expressio...

journal_title：FEBS letters

authors： Djelidi S,Fay M,Cluzeaud F,Thomas-Soumarmon A,Bonvalet JP,Farman N,Blot-Chabaud M

更新日期：1999-11-05 00:00:00

abstract：:The molecular basis and functional properties of a variant antithrombin (AT) protein. AT Budapest 3, were studied. A single base substitution was identified in codon 99, CTC----TTC, altering the normal leucine to phenylalanine. The proband presented with a history of venous thrombotic disease and was found to be homoz...

journal_title：FEBS letters

authors： Olds RJ,Lane DA,Boisclair M,Sas G,Bock SC,Thein SL

更新日期：1992-04-06 00:00:00

abstract：:Human preimplantation developmental studies are difficult to accomplish due to associated ethical and moral issues. Preimplantation cells are rare and exist only in transient cell states. From a single cell, it is very challenging to analyse the origination of the heterogeneity and complexity inherent to the human bod...

journal_title：FEBS letters

authors： Otte J,Wruck W,Adjaye J

更新日期：2017-08-01 00:00:00

abstract：:Tyrosine-rich acidic matrix protein (TRAMP; 22 kDa extracellular matrix protein; dermatopontin) is a protein that co-purifies with lysyl oxidase and with dermatan sulphate proteoglycans, with possible functions in cell-matrix interactions and matrix assembly. Using a rabbit polyclonal antiserum raised against porcine ...

journal_title：FEBS letters

authors： Forbes EG,Cronshaw AD,MacBeath JR,Hulmes DJ

更新日期：1994-09-12 00:00:00

abstract：:Two hexokinases were characterized in Schizosaccharomyces pombe: hexokinase 1, with a low phosphorylation coefficient on glucose (Km 8.5 mM) and hexokinase 2, a kinetically conventional hexokinase. Genes hxk1+ and hxk2+ encoding these enzymes were cloned and sequenced. Disruption of hxk1+ had no effect on growth but d...

journal_title：FEBS letters

authors： Petit T,Blázquez MA,Gancedo C

更新日期：1996-01-08 00:00:00

abstract：:The C-termini of p66 and p51 forms of HIV-1 reverse transcriptase have been engineered to contain a Glu-Glu-Phe sequence recognized by a monoclonal antibody to alpha-tubulin, YL1/2. Mutated RTs were purified in a single step using peptide elution from columns of immobilized YL1/2. The known sequence requirements of th...

journal_title：FEBS letters

authors： Stammers DK,Tisdale M,Court S,Parmar V,Bradley C,Ross CK

更新日期：1991-06-03 00:00:00

abstract：:Purified antibodies against guanylic acid and guanosine binding to RNA at guanosine residues were used to probe human lymphocyte preparations by indirect immunofluorescence. Neither antibody gave any banding pattern with metaphase chromosomes but both showed binding to specific sites in the interphase nuclei. Evidence...

journal_title：FEBS letters

authors： Iswari S,Krishnamurthy DS,Jacob TM

更新日期：1985-09-23 00:00:00

abstract：:The eukaryotic Ssu72 factor is involved in several RNA biogenesis processes. It has phosphatase activity on the carboxy-terminal domain (CTD) of the major subunit of RNA polymerase II. The Kluyveromyces lactis Ssu72 (KlSsu72) shows in vitro phosphatase activity for the pNPP substrate, and this activity is inhibited by...

journal_title：FEBS letters

authors： Rodríguez-Torres AM,Lamas-Maceiras M,García-Díaz R,Freire-Picos MA

更新日期：2013-08-19 00:00:00

abstract：:G protein-coupled receptor kinase 2 (GRK2) plays a key role in determining the rate and extent of G protein-coupled receptor (GPCR) desensitization and resensitization. Recent data indicate that GRK2 activity, subcellular distribution and expression are tightly regulated. The important physiological function of GRK2 a...

journal_title：FEBS letters

authors： Aragay AM,Ruiz-Gómez A,Penela P,Sarnago S,Elorza A,Jiménez-Sainz MC,Mayor F Jr

更新日期：1998-06-23 00:00:00

abstract：:The fate of newly synthesized human immunodeficiency virus type 1 env gp160 was examined in COS-1 cells. The results of morphological chase experiments involving cycloheximide demonstrated that gp160 was retained in the Golgi apparatus for longer than the half-life of the molecule. The degradation of gp160 was insensi...

journal_title：FEBS letters

authors： Kimura T,Nishikawa M,Fujisawa J

更新日期：1996-07-15 00:00:00

abstract：:Calcium channels derived from sarcoplasmic reticulum of frog skeletal muscle were fused with planar lipid bilayers. Fractional open times displayed two types of calcium dependence: (i) blockable channels showed a bell-shaped calcium dependence with an activation constant of 4.5 microM, a Hill coefficient for activatio...

journal_title：FEBS letters

authors： Bull R,Marengo JJ

更新日期：1993-10-04 00:00:00

abstract：:We present a new water selective pulse sequence allowing rapid determination of exchange rates of labile protons on the millisecond time scale. Using diffusion measurements, exchange rates of resolved protons can be determined without prior knowledge of relaxation parameters in a short overnight experiment. The use of...

journal_title：FEBS letters

authors： Böckmann A,Guittet E

更新日期：1997-11-24 00:00:00

abstract：:SP-40,40 is a two-chain serum protein which acts in vitro as a potent inhibitor of the assembly of the membrane attack complex of human complement. It contains 10 cysteine residues, the numbers and locations of which are conserved in several mammalian species. Evidence is presented that all the cysteine residues are i...

journal_title：FEBS letters

authors： Kirszbaum L,Bozas SE,Walker ID

更新日期：1992-02-03 00:00:00

abstract：:Phosphotyrosine-containing proteins in Dictyostelium discoideum were detected by immunoblot analysis and immunoprecipitation using a monoclonal anti-phosphotyrosine antibody. The iodinated antibody recognized on bots a cluster of 205-220 kDa polypeptides and bands of 107 and 60 kDa. The 107 and 60 kDa polypeptides and...

journal_title：FEBS letters

authors： Schweiger A,Mihalache O,Muhr A,Adrian I

更新日期：1990-07-30 00:00:00