Heparin binding peptides co-purify with glycosaminoglycans from human plasma.

abstract：:An experimental procedure is described for the reproducible production of hybridoma cells using the electrofusion technique. High yields can be obtained when fusion is performed in isotonic inositol solutions containing Ca2+ and Mg2+ in a ratio of 1:5 in the millimolar range. The hybridoma cells are transferred 10 min...

journal_title：FEBS letters

authors： Vienken J,Zimmermann U

更新日期：1985-03-25 00:00:00

abstract：:Apicomplexan parasites contain a vestigial plastid called apicoplast which has been suggested to be a site of [Fe-S] cluster biogenesis. Here we report the cloning of lipoic acid synthase (LipA) from Toxoplasma gondii, a well known [Fe-S] protein. It is able to complement a LipA-deficient Escherichia coli strain, clea...

journal_title：FEBS letters

authors： Thomsen-Zieger N,Schachtner J,Seeber F

更新日期：2003-07-17 00:00:00

abstract：:Human synovium was analyzed for the possible expression of cartilage oligomeric matrix protein (COMP). Immunostaining with polyclonal antiserum to COMP demonstrated positive staining within the synovial cells and immediately subjacent connective tissue, with less intense staining in the deeper connective tissue. Weste...

journal_title：FEBS letters

authors： Di Cesare PE,Carlson CS,Stollerman ES,Chen FS,Leslie M,Perris R

更新日期：1997-07-21 00:00:00

abstract：:α-Helical membrane-active antimicrobial peptides (AMPs) are known to act via a range of mechanisms, including the formation of barrel-stave and toroidal pores and the micellisation of the membrane (carpet mechanism). Different mechanisms imply that the peptides adopt different 3D structures when bound at the water-mem...

journal_title：FEBS letters

authors： van den Bergen G,Stroet M,Caron B,Poger D,Mark AE

更新日期：2020-03-01 00:00:00

abstract：:Mona is an SH3 and SH2 domain-containing adapter molecule that is induced during monocytic differentiation. Here we have first shown that M-CSFR is the major Mona partner in M-CSF signaling, the interaction being mediated through tyrosine 697 of the receptor. Next we asked whether Mona expression would alter the Ras/M...

journal_title：FEBS letters

authors： Bourgin C,Bourette R,Mouchiroud G,Arnaud S

更新日期：2000-09-01 00:00:00

abstract：:Cytosine in nucleic acids can be converted into N4-aminocytosine by treatment with a mixture of hydrazine and bisulfite. The hydrazino group thus formed at position 4 of the pyrimidine ring can be linked to a sulhydryl group in proteins by the use of bromopyruvate as a linker. Successful use of this scheme of chemical...

journal_title：FEBS letters

authors： Nitta N,Kuge O,Yui S,Tsugawa A,Negishi K,Hayatsu H

更新日期：1984-01-23 00:00:00

abstract：:Resistance to antimalarial drugs has often threatened malaria elimination efforts and historically has led to the short-term resurgence of malaria incidences and deaths. With concentrated malaria eradication efforts currently underway, monitoring drug resistance in clinical settings complemented by in vitro drug susce...

journal_title：FEBS letters

authors： Petersen I,Eastman R,Lanzer M

更新日期：2011-06-06 00:00:00

abstract：:Phospholamban (PLB), an integral membrane protein of cardiac sarcoplasmic reticulum (SR), is described as the regulator of the Ca(2+)-ATPase pump, via its phosphorylation-dephosphorylation of Ser-16. Recently it has been shown that a direct interaction between the N-terminal hydrophilic domain of PLB and Ca(2+)-ATPase...

journal_title：FEBS letters

authors： Terzi E,Poteur L,Trifilieff E

更新日期：1992-09-14 00:00:00

abstract：:This study describes the comparative analysis of two insect recombinant aminotransferases, Aedes aegypti 3-hydroxykynurenine (3-HK) transaminase/alanine glyoxylate aminotransferase (Ae-HKT/AGT) and Drosophila melanogaster serine pyruvate aminotransferase (Dm-Spat), which share 52% identity in their amino acid sequence...

journal_title：FEBS letters

authors： Han Q,Li J

更新日期：2002-09-11 00:00:00

abstract：:Homocysteine thiolactone is a product of an error-editing reaction, catalyzed by Escherichia coli and Saccharomyces cerevisiae methionyl-tRNA synthetases, which prevents incorporation of homocysteine into tRNA and protein both in vitro and in vivo. Here, homocysteine thiolactone is also shown to be synthesized by cult...

journal_title：FEBS letters

authors： Jakubowski H,Goldman E

更新日期：1993-02-15 00:00:00

abstract：:Several myeloid precursors of antibacterial peptides have recently been shown to share homologous pre- and pro-regions. Taking advantage of this homology, a novel cDNA was cloned from pig bone marrow RNA. This encodes a 166-residue polypeptide with highly conserved pre- (29 residues) and pro- (101 residues) sequences,...

journal_title：FEBS letters

authors： Storici P,Scocchi M,Tossi A,Gennaro R,Zanetti M

更新日期：1994-01-17 00:00:00

abstract：:Regulated permeability changes have been detected in mitochondria across species. We review here their key features, with the goal of assessing whether a "permeability transition" similar to that observed in higher eukaryotes is present in other species. The recent discoveries (i) that treatment with cyclosporin A (Cs...

journal_title：FEBS letters

authors： Azzolin L,von Stockum S,Basso E,Petronilli V,Forte MA,Bernardi P

更新日期：2010-06-18 00:00:00

abstract：:The T-cell antigen receptor (TCR) is a multisubunit transmembrane complex that mediates the antigen-specific activation of T cells. Using a variety of techniques, several research groups have shown that TCRs are at least partially pre-clustered before antigen binding. These new findings are contradictory to the "class...

journal_title：FEBS letters

authors： Molnár E,Deswal S,Schamel WW

更新日期：2010-12-15 00:00:00

abstract：:The delta psi of R. sphaeroides, grown under high light to reduce the levels of light-harvesting bacteriochlorophyll, was naturally manipulated using light intensity. The relationship between delta psi and the swimming speed of free swimming populations of cells was investigated. After de-energisation by incubation in...

journal_title：FEBS letters

authors： Harrison DM,Packer HL,Armitage JP

更新日期：1994-07-04 00:00:00

abstract：:Our data show that in hamster fibroblasts transformed by Rous sarcoma virus (RSV), the phosphoinositide 3'-kinase (PI-3K)/Akt/glycogen synthase kinase 3 antiapoptotic pathway is upregulated and involved in increased protein synthesis through activation of initiation factor eIF2B. Upon inhibition of PI-3K by wortmannin...

journal_title：FEBS letters

authors： Vojtechová M,Sloncová E,Kucerová D,Jiricka J,Sovová V,Tuhácková Z

更新日期：2003-05-22 00:00:00

abstract：:The possibility that the phospholipid-N-methyltransferases from yeast are capable of acting upon a phospholipid substrate, localized in a different membrane than in which the enzymes reside ('trans-catalysis' hypothesis), was investigated using cho2 and opi3 gene disruptant strains, which are defective in phosphatidyl...

journal_title：FEBS letters

authors： Janssen MJ,de Jong HM,de Kruijff B,de Kroon AI

更新日期：2002-02-27 00:00:00

abstract：:A role for the cytokines produced by tissue-infiltrated inflammatory cells (mainly T-lymphocytes and mast cells) in the pathophysiology of fibrosis has been suggested by several groups. Among the products of these cells, interleukin-4 (IL-4) might be one of the factors involved in the initiation of the fibrotic proces...

journal_title：FEBS letters

authors： Gillery P,Fertin C,Nicolas JF,Chastang F,Kalis B,Banchereau J,Maquart FX

更新日期：1992-05-18 00:00:00

abstract：:We have previously shown that Rap1 as well as its guanine nucleotide exchange factor Epac1 increases cell-cell junction formation. Here, we show that activation of Epac1 with the exchange protein directly activated by cAMP (Epac)-specific cAMP analog 8CPT-2'O-Me-cAMP (007) resulted in a tightening of the junctions and...

journal_title：FEBS letters

authors： Kooistra MR,Corada M,Dejana E,Bos JL

更新日期：2005-09-12 00:00:00

abstract：:The effect of Rhodococcus fascians, the causal agent of leafy gall disease, on the mitotic behavior of synchronized tobacco Bright Yellow-2 (BY-2) cells was investigated. Incubation of aphidicolin-synchronized BY-2 cells with R. fascians cells specifically resulted in a broader mitotic index peak, an effect that was l...

journal_title：FEBS letters

authors： Temmerman W,Ritsema T,Simón-Mateo C,Van Montagu M,Mironov V,Inzé D,Goethals K,Holsters M

更新日期：2001-03-09 00:00:00

abstract：:D-aspartate and human chorionic gonadotropin act synergistically to increase testosterone production in purified rat Leydig cells, and D-aspartate stimulates testosterone synthesis even in the absence of human chorionic gonadotropin stimulation. In addition, D-aspartate enhances steady-state cellular mRNA and protein ...

journal_title：FEBS letters

authors： Nagata Y,Homma H,Matsumoto M,Imai K

更新日期：1999-07-09 00:00:00

abstract：:We have used a competitive enzyme-linked immunoassay with a panel of monoclonal antibodies to probe the topography of the membrane-bound form of apolipoprotein B (apo B) in rabbit microsomes. All epitopes investigated were found to be expressed at the cytosolic side of the microsomal membrane under conditions in which...

journal_title：FEBS letters

authors： Wilkinson J,Higgins JA,Groot PH,Gherardi E,Bowyer DE

更新日期：1992-06-08 00:00:00

abstract：:The concentration of caffeine required to elute wholly single-stranded DNA from benzoylated DEAE-cellulose is proportional to the polynucleotide length. The use of benzoylated DEAE-cellulose chromatography for isolating and sizing single-stranded regions in double-stranded DNA has been examined using a series of hybri...

journal_title：FEBS letters

authors： Norris MD,Stewart BW

更新日期：1988-02-15 00:00:00

abstract：:The sialic acid of complex N-glycans can be biochemically engineered by substituting the physiological precursor N-acetylmannosamine with non-natural N-acylmannosamines. The Kv3.1 glycoprotein, a neuronal voltage-gated potassium channel, contains sialic acid. Western blots of the Kv3.1 glycoprotein isolated from trans...

journal_title：FEBS letters

authors： Hall MK,Reutter W,Lindhorst T,Schwalbe RA

更新日期：2011-10-20 00:00:00

abstract：:Stresses that impair the function of the endoplasmic reticulum (ER) lead to an accumulation of unfolded protein in the ER. Under these conditions, the expression of a variety of genes involved in preventing the accumulation of the unfolded proteins is induced. Yeast Hrd1p is an ER stress-inducible ER membrane protein ...

journal_title：FEBS letters

authors： Kaneko M,Ishiguro M,Niinuma Y,Uesugi M,Nomura Y

更新日期：2002-12-04 00:00:00

abstract：:The expression of interphotoreceptor retinoid binding protein (IRBP) is limited to photoreceptor cells of the retina and pinealocytes of the pineal gland. We sought to define cis-elements of the mouse IRBP 5' flanking region that are required for this restricted activity. In vitro transient transfections of retinoblas...

journal_title：FEBS letters

authors： Boatright JH,Knox BE,Jones KM,Stodulkova E,Nguyen HT,Padove SA,Borst DE,Nickerson JM

更新日期：2001-08-24 00:00:00

abstract：:We have attempted to elucidate the mechanism of apoptotic cell death induced by hypoxia (very low oxygen conditions) in neuronal cells. Human neuroblastoma SK-N-MC cells under hypoxic conditions resulted in apoptosis in a time-dependent manner estimated by DNA fragmentation assay and nuclear morphology stained with fl...

journal_title：FEBS letters

authors： Araya R,Uehara T,Nomura Y

更新日期：1998-11-13 00:00:00

abstract：:The photocycle of the light-adapted purple membrane was studied with a time-resolved fluorometry apparatus: fluorescence of the sample suspension (greater than 660 nm) was pumped with a 633-nm cw laser and the temporal change induced by a 532-nm pulsed laser was measured with a photon-counting-type transient recorder....

journal_title：FEBS letters

authors： Ohtani H,Itoh H,Shinmura T

更新日期：1992-06-22 00:00:00

abstract：:Scorpion toxins have been found lacking effect on Na(+) current of its own sodium channel, whereas the molecular mechanism remains mystery. In this study, the binding affinity of pharmacologically distinct scorpion toxins was found much weaker to scorpion (Buthus martensii) nerve synaptosomes than to spider (Ornithoct...

journal_title：FEBS letters

authors： Zuo XP,He HQ,He M,Liu ZR,Xu Q,Ye JG,Ji YH

更新日期：2006-08-07 00:00:00

abstract：:The human gut microbiota comprises approximately 100 trillion microbial cells and has a significant effect on many aspects of human physiology including metabolism, nutrient absorption and immune function. Disruption of this population has been implicated in many conditions and diseases, including examples such as obe...

journal_title：FEBS letters

authors： Walsh CJ,Guinane CM,O'Toole PW,Cotter PD

更新日期：2014-11-17 00:00:00

abstract：:Random sequences of 120-130 amino acid residues were inserted into a surface loop region of Escherichia coli RNase HI. This library was screened and about 10% of the clones were found to retain RNase H activity. Subsequent random mutagenesis led to an increase in RNase H activity and solubility of the protein. The ins...

journal_title：FEBS letters

authors： Doi N,Itaya M,Yomo T,Tokura S,Yanagawa H

更新日期：1997-02-03 00:00:00