Abstract:
:Our data show that in hamster fibroblasts transformed by Rous sarcoma virus (RSV), the phosphoinositide 3'-kinase (PI-3K)/Akt/glycogen synthase kinase 3 antiapoptotic pathway is upregulated and involved in increased protein synthesis through activation of initiation factor eIF2B. Upon inhibition of PI-3K by wortmannin, phosphorylation of 70-kDa ribosomal protein S6 kinase (p70 S6k) and its physiological substrate, ribosomal protein S6, decreased in the non-transformed cells but not in RSV-transformed cells. Thus PI-3K, which is thought to be involved in regulation of p70 S6k, signals to p70 S6k in normal fibroblasts, but it does not appear to be an upstream effector of p70 S6k in fibroblasts transformed by v-src oncogene, suggesting that changes in the PI-3K signalling pathway upstream of p70 S6k are induced by RSV transformation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Vojtechová M,Sloncová E,Kucerová D,Jiricka J,Sovová V,Tuhácková Zdoi
10.1016/s0014-5793(03)00415-0keywords:
subject
Has Abstractpub_date
2003-05-22 00:00:00pages
81-6issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579303004150journal_volume
543pub_type
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