Membrane-bound apolipoprotein B is exposed at the cytosolic surface of liver microsomes.

abstract：:A full-length cDNA coding for troponin-C isolated from an adult rabbit fast skeletal muscle library has been sequenced and expressed in an E. coli host. The amino acid sequence derived from the coding region agrees with the published protein sequence except that the first two residues are reversed. The expressed prote...

journal_title：FEBS letters

authors： Chen Q,Taljanidisz J,Sarkar S,Tao T,Gergely J

更新日期：1988-02-08 00:00:00

abstract：:Translocation of folded proteins by the Tat system of Escherichia coli is believed to rely on the presence of phosphatidylethanolamine (PE) and the negatively charged phospholipids cardiolipin (CL) and phosphatidylglycerol (PG). Here, we show that while PE is indeed essential for activity, the Tat system is fully func...

journal_title：FEBS letters

authors： Rathmann C,Schlösser AS,Schiller J,Bogdanov M,Brüser T

更新日期：2017-09-01 00:00:00

abstract：:The present study was focused on the regulation of ecdysteroidogenesis in the Y-organ of Scylla serrata during molting cycle. A strong expression of molt-inhibiting hormone (MIH) and phosphorylation of ERK was predominantly observed in the postmolt and intermolt stages of Y-organs, whereas protein kinase C, steroidoge...

journal_title：FEBS letters

authors： Imayavaramban L,Dhayaparan D,Devaraj H

更新日期：2007-11-13 00:00:00

abstract：:Vascular smooth muscle cell (VSMC) proliferation plays a key role in neointimal hyperplasia and restenosis. Here we report the role of the microRNA miR-142-5p and its downstream target genes on the proliferation of cultured VSMCs. miR-142-5p promoted VSMC proliferation by down-regulating B cell translocation gene 3 (B...

journal_title：FEBS letters

authors： Kee HJ,Park S,Kwon JS,Choe N,Ahn Y,Kook H,Jeong MH

更新日期：2013-08-02 00:00:00

abstract：:VirG, PhoB, and OmpR are positive regulators of the virulence, phosphate, and osmolarity regulons, respectively. Their target genes are attended with one or more significant blocks of similar 6-bp sequences in their regulatory regions. The distance between the block(s) and transcriptional start site varies with the ta...

journal_title：FEBS letters

authors： Aoyama T,Oka A

更新日期：1990-04-09 00:00:00

abstract：:Mediatophore is a protein purified from the nerve terminal membranes of Torpedo electric organ. It confers to artificial membranes a calcium-dependent mechanism that translocates acetylcholine. When similar reconstitution experiments are applied to rat brain synaptosomal membranes they reveal the presence of mediatoph...

journal_title：FEBS letters

authors： Israël M,Lesbats B,Morel N,Manaranche R,Le Gal la Salle G

更新日期：1988-06-20 00:00:00

abstract：:NADP-malic enzyme (NADP-ME) is a widely distributed enzyme that catalyzes the oxidative decarboxylation of L-malate. Photosynthetic NADP-MEs are found in C4 bundle sheath chloroplasts and in the cytosol of CAM plants, while non-photosynthetic NADP-MEs are either plastidic or cytosolic in various plants. We propose a c...

journal_title：FEBS letters

authors： Drincovich MF,Casati P,Andreo CS

更新日期：2001-02-09 00:00:00

abstract：:With the aging of population, neurological disorders, and especially disorders involving defects in protein conformation (also known as proteopathies) pose a serious socio-economic problem. So far there is no effective treatment for most proteopathies, including Parkinson's disease (PD). The mechanism underlying PD pa...

journal_title：FEBS letters

authors： Oláh J,Ovádi J

更新日期：2019-07-01 00:00:00

abstract：:The binding of folate to Lactobacillus casei dihydrofolate reductase in the presence and absence of NADP+ has been studied by 15N NMR, using [5-15N]folate. In the presence of NADP+, three separate signals were observed for the single 15N atom, in agreement with our earlier evidence from 1H and 13C NMR for multiple con...

journal_title：FEBS letters

authors： Birdsall B,De Graw J,Feeney J,Hammond S,Searle MS,Roberts GC,Colwell WT,Crase J

更新日期：1987-06-08 00:00:00

abstract：:ADP-forming acetyl-CoA synthetase (ACD) catalyzes the interconversion of acetyl-CoA and acetate. The related succinyl-CoA synthetase follows a three-step mechanism involving a single phosphoenzyme, but a novel four-step mechanism with two phosphoenzyme intermediates was proposed for Pyrococcus ACD. Characterization of...

journal_title：FEBS letters

authors： Jones CP,Khan K,Ingram-Smith C

更新日期：2017-02-01 00:00:00

abstract：:Thioredoxins are a class of small proteins that play a key role in regulating many cellular redox processes. We report here the characterization of the first member of the thioredoxin family in metazoans that is mainly associated with neurons. The Caenorhabditis elegans gene B0228.5 encodes a thioredoxin (TRX-1) that ...

journal_title：FEBS letters

authors： Miranda-Vizuete A,Fierro González JC,Gahmon G,Burghoorn J,Navas P,Swoboda P

更新日期：2006-01-23 00:00:00

abstract：:The expression of rat brain voltage-sensitive Na+ channel mRNAs in Schwann cells was examined using in situ hybridization cytochemistry and RT-PCR. The mRNAs of rat brain Na+ channel subtype II and III, but not subtype I, were detected in cultured Schwann cells from sciatic nerve and in intact sciatic nerve, which con...

journal_title：FEBS letters

authors： Oh Y,Black JA,Waxman SG

更新日期：1994-08-22 00:00:00

abstract：:The purpose of this investigation was to study the effect on cap structure for trans-splicing in mammalian cells. The early SV40 Bst/Bam pre-mRNA (cRNA) was synthesized in vitro in both capped (cap-Bst/Bam-cRNA) and non-capped (Bst/Bam-cRNA) versions and microinjected into the nuclei of TC7 cells. Trans-splicing was m...

journal_title：FEBS letters

authors： Graessmann M,Eul J,Berg B,Zimmermann C,Graessmann A

更新日期：1996-09-30 00:00:00

abstract：:In developing ideas of how protein structure modifies haem reactivity, the activity of Class I of the plant peroxidase superfamily (including cytochrome c peroxidase, ascorbate peroxidase and catalase-peroxidases (KatGs)) is an exciting field of research. Despite striking sequence homologies, there are dramatic differ...

journal_title：FEBS letters

authors： Zámocký M,Regelsberger G,Jakopitsch C,Obinger C

更新日期：2001-03-16 00:00:00

abstract：:The possible role of sphingomyelin cycle for the regulation of cell proliferation was investigated in human keratinocytes. The time-dependent breakdown of sphingomyelin was observed in the immortalized human keratinocyte cell line HaCaT as well as in primary human keratinocytes thereby providing evidence that the sphi...

journal_title：FEBS letters

authors： Geilen CC,Bektas M,Wieder T,Orfanos CE

更新日期：1996-01-02 00:00:00

abstract：:The cyclic AMP phosphodiesterases type 4 (PDE4s) are expressed in a cell specific manner, with intracellular targeting directed by unique N-terminal anchor domains. All long form PDE4s are phosphorylated and activated by PKA phosphorylation within their upstream conserved region 1 (UCR1). Here, we identify and charact...

journal_title：FEBS letters

authors： Byrne AM,Elliott C,Hoffmann R,Baillie GS

更新日期：2015-03-12 00:00:00

abstract：:Protease involvement has been implicated in the signalling process of activation-induced apoptosis. Here we report the isolation of a protease from Jurkat T cells undergoing Fas-induced apoptosis. Although the protease probably is a serine protease, it seems to be distantly related to members of the ICE/ced-3/Ich-1(ne...

journal_title：FEBS letters

authors： Schlegel J,Peters I,Orrenius S

更新日期：1995-05-08 00:00:00

abstract：:Using sensitive methods of sequence analysis including hydrophobic cluster analysis, we report here a hitherto undescribed family of modules, the BAH (bromo-adjacent homology) family, which includes proteins such as eukaryotic DNA (cytosine-5) methyltransferases, the origin recognition complex 1 (Orc1) proteins, as we...

journal_title：FEBS letters

authors： Callebaut I,Courvalin JC,Mornon JP

更新日期：1999-03-05 00:00:00

abstract：:The sulfoxide Ro 18-5364, a potential metabolite of the IND Ro 18-5362, is a powerful inhibitor of gastric mucosal (H+ + K+)-ATPase, decreasing enzymatic activity with an apparent Ki of 0.1 microM. Exposure of Ro 18-5364-treated gastric membranes to dithiothreitol fully restored (H+ + K+)-ATPase activity. ATP protecte...

journal_title：FEBS letters

authors： Sigrist-Nelson K,Müller RK,Fischli AE

更新日期：1986-03-03 00:00:00

abstract：:The interactions between the polyanionic ligands phosphate and sulphate and the type II dehydroquinases from Streptomyces coelicolor and Mycobacterium tuberculosis have been characterised using a combination of structural and kinetic methods. From both approaches, it is clear that interactions are more complex in the ...

journal_title：FEBS letters

authors： Evans LD,Roszak AW,Noble LJ,Robinson DA,Chalk PA,Matthews JL,Coggins JR,Price NC,Lapthorn AJ

更新日期：2002-10-23 00:00:00

abstract：:ZAP-70 is a protein tyrosine kinase (PTK) that plays a critical role in T cell activation. To study the role of ZAP-70 catalytic activity in this process, a substrate capable of distinguishing between the activities of ZAP-70 and other PTKs would be useful, especially since it has recently been shown that ZAP-70 inter...

journal_title：FEBS letters

authors： Watts JD,Brabb T,Bures EJ,Wange RL,Samelson LE,Aebersold R

更新日期：1996-12-02 00:00:00

abstract：:One of the main objectives in the analysis of microarray experiments is the identification of genes that are differentially expressed under two experimental conditions. This task is complicated by the noisiness of the data and the large number of genes that are examined simultaneously. Here, we present a novel techniq...

journal_title：FEBS letters

authors： Breitling R,Armengaud P,Amtmann A,Herzyk P

更新日期：2004-08-27 00:00:00

abstract：:Phosphorylations catalyzed by cAMP-dependent protein kinase are essential for sperm motility, and type II cAMP-dependent protein kinase in mature sperm has been shown to be firmly bound to the flagellum via the regulatory subunit, RII. The present study documents high-levelled expression of a human, testis-specific RI...

journal_title：FEBS letters

authors： Oyen O,Myklebust F,Scott JD,Hansson V,Jahnsen T

更新日期：1989-03-27 00:00:00

abstract：:The fluorescent Ca2+ indicator Fura 2 was used to monitor Ca2+ release induced by the Ins(1,4,5)P3-mobilizing agonist angiotensin II (Ag II), caffeine and 2,5-di-(tert-butyl)-1,4-benzohydroquinone (tuBHQ), in intact bovine adrenal chromaffin cells. Under low external Ca2+ conditions, tuBHQ, Ag II and caffeine elicited...

journal_title：FEBS letters

authors： Robinson IM,Burgoyne RD

更新日期：1991-09-09 00:00:00

abstract：:The effect of caffeine on catecholamine secretion and intracellular free Ca2+ concentration [( Ca2+]i) in bovine adrenal chromaffin cells was examined using single fura-2-loaded cells and cell populations. In cell populations caffeine elicited a large (approximately 200 nM) transient rise in [Ca2+]i that was independe...

journal_title：FEBS letters

authors： Cheek TR,O'Sullivan AJ,Moreton RB,Berridge MJ,Burgoyne RD

更新日期：1990-06-18 00:00:00

abstract：:We have studied the intracellular distribution in vivo of glucokinase (GK) and glucokinase regulatory protein (GKRP) in livers of fasted and refed rats, using specific antibodies against both proteins and laser confocal fluorescence microscopy. GK was found predominantly in the nucleus of hepatocytes from starved rats...

journal_title：FEBS letters

authors： Fernández-Novell JM,Castel S,Bellido D,Ferrer JC,Vilaró S,Guinovart JJ

更新日期：1999-10-08 00:00:00

abstract：:The covalent binding of pyridoxal 5'-phosphate (PLP) to human serum albumin (HSA) is important in the regulation of PLP metabolism. In plasma, PLP is bound to HSA at a single high-affinity and at two or more nonspecific sites. To characterize the primary PLP binding site, HSA was incubated with [3H] PLP, and the Schif...

journal_title：FEBS letters

authors： Bohney JP,Fonda ML,Feldhoff RC

更新日期：1992-02-24 00:00:00

abstract：:The structure of GE82832, a translocation inhibitor produced by a soil microorganism, is shown to be highly related to that of dityromycin, a bicyclodecadepsipeptide antibiotic discovered long ago whose characterization had never been pursued beyond its structural elucidation. GE82832 and dityromycin were shown to int...

journal_title：FEBS letters

authors： Brandi L,Maffioli S,Donadio S,Quaglia F,Sette M,Milón P,Gualerzi CO,Fabbretti A

更新日期：2012-09-21 00:00:00

abstract：:We report a nonenzymatic reaction which results in the radiolabeling of proteins by 32P-containing nucleoside triphosphates. The labeling reaction does not require any cofactors, but is greatly enhanced by the presence of alcohols. Even under optimal conditions, less than 1% of the protein molecules undergo modificati...

journal_title：FEBS letters

authors： Schmidt MC,Hanna MM

更新日期：1986-01-06 00:00:00

abstract：:Protein kinase A (PKA) signaling is essential for growth and virulence of the fungal pathogen Aspergillus fumigatus. Little is known concerning the regulation of this pathway in filamentous fungi. Employing liquid chromatography-tandem mass spectroscopy, we identified novel phosphorylation sites on the regulatory subu...

journal_title：FEBS letters

authors： Shwab EK,Juvvadi PR,Waitt G,Soderblom EJ,Moseley MA,Nicely NI,Steinbach WJ

更新日期：2017-11-01 00:00:00