Intracellular distribution of hepatic glucokinase and glucokinase regulatory protein during the fasted to refed transition in rats.

abstract：:Two protein phosphatases of 103 and 29 kDa as determined by gel filtration, were purified from hen oviducts. The 103 -kDa phosphatase was purified 7300-fold to near homogeneity and dissociated into two polypeptides in the presence of SDS. Molecular masses of these polypeptides were estimated to be 60 and 38 kDa by SDS...

journal_title：FEBS letters

authors： Kanayama K,Wada K,Negami A,Yamamura H,Tanabe T

更新日期：1985-05-06 00:00:00

abstract：:The dinuclear paramagnetic center of the soluble CuA domain of the cytochrome c oxidase from Bacillus subtilis has been studied using 1H NMR. The spectrum possesses remarkably sharp shifted resonances. Comparison with the spectrum of the CuA amicyanin variant provides the spin density distribution in the CuA site of c...

journal_title：FEBS letters

authors： Dennison C,Berg A,de Vries S,Canters GW

更新日期：1996-10-07 00:00:00

abstract：:Cytosolic sialidase Neu2 has been implicated in myoblast differentiation. Here we observed a significant upregulation of Neu2 expression during differentiation of murine C2C12 myoblasts. This was evidenced both as an increase in Neu2 mRNA steady-state levels and in the cytosolic sialidase enzymatic activity. To unders...

journal_title：FEBS letters

authors： Fanzani A,Giuliani R,Colombo F,Zizioli D,Presta M,Preti A,Marchesini S

更新日期：2003-07-17 00:00:00

abstract：:The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation...

journal_title：FEBS letters

authors： Olsen J,Laustsen L,Troelsen J

更新日期：1994-04-11 00:00:00

abstract：:BIR domain and its containing proteins play critical roles in cell apoptosis and cell division. Here several lines of novelty were revealed based on a comprehensive evolutionary analysis of BIR domains in 11 representative organisms. First, the type II BIR domains in Survivin and Bruce showed more conservation compare...

journal_title：FEBS letters

authors： Cao L,Wang Z,Yang X,Xie L,Yu L

更新日期：2008-11-12 00:00:00

abstract：:The Surfeit locus is an unusual cluster of at least 6 housekeeping genes whose organisation is conserved between birds and mammals. We have previously shown that the divergently transcribed Surf-1 and Surf-2 genes are separated by a bi-directional, TATA-less promoter. In mouse, the Surf-1/Surf-2 promoter contains thre...

journal_title：FEBS letters

authors： Gaston K,Fried M

更新日期：1994-06-27 00:00:00

abstract：:We present evidence that gastrin, binding to a G protein-coupled receptor, activates the p38-mitogen-activated protein kinase (MAPK) pathway. Blockage of protein kinase C (PKC) by GF109203X, depletion of intracellular calcium by thapsigargin or inhibition of Src family kinases by PP2 prevented p38-MAPK activation and ...

journal_title：FEBS letters

authors： Dehez S,Daulhac L,Kowalski-Chauvel A,Fourmy D,Pradayrol L,Seva C

更新日期：2001-05-04 00:00:00

abstract：:Renin, which catalyzes the initial proteolytic cleavage reaction in the production of angiotensins, is first synthesized as a zymogen, prorenin, and requires the proteolytic removal of an amino-terminal prosegment for activation in vivo. The lysosomal hydrolase cathepsin B has been proposed as a prorenin processing en...

journal_title：FEBS letters

authors： Jutras I,Reudelhuber TL

更新日期：1999-01-22 00:00:00

abstract：:NELL1 is an extracellular protein inducing osteogenic differentiation and bone formation of osteoblastic cells. To elucidate the intracellular signaling cascade evoked by NELL1, we have shown that NELL1 protein transiently activates the MAPK signaling cascade, induces the phosphorylation of Runx2, and promotes the rap...

journal_title：FEBS letters

authors： Bokui N,Otani T,Igarashi K,Kaku J,Oda M,Nagaoka T,Seno M,Tatematsu K,Okajima T,Matsuzaki T,Ting K,Tanizawa K,Kuroda S

更新日期：2008-01-23 00:00:00

abstract：:Adequate dietary intake of essential metals such as zinc is important for maintaining homeostasis. Abnormal zinc intake in Caenorhabditis elegans has been shown to increase or decrease normal lifespan by influencing the insulin/IGF-1 pathway. Distribution of zinc is achieved by a family of highly conserved zinc transp...

journal_title：FEBS letters

authors： Novakovic S,Molesworth LW,Gourley TE,Boag PR,Davis GM

更新日期：2020-05-01 00:00:00

abstract：:Vasopressin elicited a dose-dependent inhibition of glucagon-induced cAMP accumulation in isolated hepatocytes. This response was not diminished by incubation of cells with the calmodulin antagonists trifluoperazine or chlorpromazine and was only slightly reduced in Ca2+-depleted hepatocytes. Half-maximal inhibition o...

journal_title：FEBS letters

authors： Morgan NG,Shipp CC,Exton JH

更新日期：1983-11-14 00:00:00

abstract：:Muscarinic K+ channels (IK(ACh)) in native atrial myocytes are activated by betagamma subunits of pertussis toxin (Ptx)-sensitive heterotrimeric G proteins coupled to different receptors. betagamma subunits of Ptx-insensitive Gs, coupled to beta-adrenergic receptors, do not activate native IK(ACh). In atrial myocytes ...

journal_title：FEBS letters

authors： Bender K,Wellner-Kienitz MC,Meyer T,Pott L

更新日期：1998-11-13 00:00:00

abstract：:The relationship among the real-time trafficking of lung metastatic B16BL6 cells, metastatic potential, and the injected number of the cells was examined, since the smaller the number of tumor cells injected, the more clearly the immune defense may be observed. When 1x10(6) or 1x10(5) B16BL6 cells were injected into m...

journal_title：FEBS letters

authors： Kikkawa H,Imafuku H,Tsukada H,Oku N

更新日期：2000-02-11 00:00:00

abstract：:New polypeptide components have been isolated from Dendroaspis angusticeps venom using chromatography. Two polypeptides containing 59 and 57 amino acids, called 'DaE1' and 'DaE2' respectively, have been purified to homogeneity and fully sequenced. Spectrometric analysis yielded masses of 6631.5 and 6389.0 Da, respecti...

journal_title：FEBS letters

authors： Tytgat J,Vandenberghe I,Ulens C,Van Beeumen J

更新日期：2001-03-02 00:00:00

abstract：:Lysosomal/phagosomal membranes of mammalian cells are coated by highly conserved glycoproteins (lamps) that are thought to protect the membranes from degradation. Interestingly, we identified an amino acid sequence in human lamp-1 characteristic of a cleavage site for the Neisseria gonorrhoeae IgA1 protease. Furthermo...

journal_title：FEBS letters

authors： Hauck CR,Meyer TF

更新日期：1997-03-17 00:00:00

abstract：:Nuclear pore complexes (NPC) form nuclear pores that cross the nuclear envelope and allow molecules to transport between the nucleus and the cytoplasm. We solved the crystal structure of human Nup43 (hNUP43), an important component in the Nup107 subcomplex of NPC. hNup43 adopts a seven-bladed β-propeller fold. We conf...

journal_title：FEBS letters

authors： Xu C,Li Z,He H,Wernimont A,Li Y,Loppnau P,Min J

更新日期：2015-10-24 00:00:00

abstract：:The isocitrate lyase-encoding gene AgICL1 from the filamentous hemiascomycete Ashbya gossypii was isolated by heterologous complementation of a Saccharomyces cerevisiae icl1d mutant. The open reading frame of 1680 bp encoded a protein of 560 amino acids with a calculated molecular weight of 62584. Disruption of the Ag...

journal_title：FEBS letters

authors： Maeting I,Schmidt G,Sahm H,Revuelta JL,Stierhof YD,Stahmann KP

更新日期：1999-02-05 00:00:00

abstract：:Formation of tubulin/Na(+),K(+)-ATPase (NKA) complex in erythrocytes of hypertensive subjects results in a 50% reduction in NKA activity. We demonstrate here that detyrosinated tubulin, which is increased in hypertensive erythrocytes membranes, enhances the inhibitory effect of acetylated tubulin on NKA activity. More...

journal_title：FEBS letters

authors： Amaiden MR,Santander VS,Monesterolo NE,Nigra AD,Rivelli JF,Campetelli AN,Pie J,Casale CH

更新日期：2015-01-30 00:00:00

abstract：:The photosynthetic reaction center is one of the most complicated molecular complexes. Transducing photon energy to a transmembrane electrochemical potential difference for protons, it is the direct or indirect energy source for virtually all life. We show here that it operates in a simple, battery-like manner, with a...

journal_title：FEBS letters

authors： van Rotterdam BJ,Crielaard W,van Stokkum IH,Hellingwerf KJ,Westerhoff HV

更新日期：2002-01-02 00:00:00

abstract：:The human ether-a-go-go-related gene (herg) encodes a K+ current (I(HERG)) which plays a fundamental role in heart excitability and in neurons by contributing to action potential repolarization and to spike-frequency adaptation, respectively. In this paper we show that I(HERG), recorded in neuroblastoma cells and guin...

journal_title：FEBS letters

authors： Rosati B,Rocchetti M,Zaza A,Wanke E

更新日期：1998-11-27 00:00:00

abstract：:Calpain, a Ca2+-dependent biomodulator, alters the properties of substrate proteins by cleaving them at a limited number of specific sites. Recent studies of the structure-function relationship of calpain and X-ray analysis of its Ca2+-binding domain have revealed hitherto unknown features of the regulation of calpain...

journal_title：FEBS letters

authors： Suzuki K,Sorimachi H

更新日期：1998-08-14 00:00:00

abstract：:In plant organelle genomes, homeologous recombination between heteroallelic positions of repetitive sequences is increased by dysfunction of the gene encoding MutS homolog 1 (MSH1), a plant organelle-specific homolog of bacterial mismatch-binding protein MutS1. The C-terminal region of plant MSH1 contains the GIY-YIG ...

journal_title：FEBS letters

authors： Fukui K,Harada A,Wakamatsu T,Minobe A,Ohshita K,Ashiuchi M,Yano T

更新日期：2018-12-01 00:00:00

abstract：:OmpC, a surface antigen of Salmonella typhi was crystallized after several attempts, using PEG 3350. Well shaped hexagonal crystals were grown from vapor diffusion method using octyl glucoside and C12E9 as detergents. Crystals are sensitive to X-ray and diffract weakly up to 7 A. Porin isoforms, due to the bound lipop...

journal_title：FEBS letters

authors： Arockiasamy A,Krishnaswamy S

更新日期：1999-06-25 00:00:00

abstract：:A novel antimicrobial peptide, named misgurin, was isolated and characterized from the loach (mudfish), Misgurnus anguillicaudatus. The 21-amino-acid peptide with a molecular mass of 2502 Da was purified to homogeneity using a heparin-affinity column and C18 reverse-phase and gel-permeation high-performance liquid chr...

journal_title：FEBS letters

authors： Park CB,Lee JH,Park IY,Kim MS,Kim SC

更新日期：1997-07-14 00:00:00

abstract：:A recombinant expression system was developed to analyse sequence determinants involved in O-glycosylation of proteins in mycobacteria. By expressing peptide sequences corresponding to known glycosylation sites within a chimeric lipoprotein construct, amino acids flanking modified threonine residues were found to have...

journal_title：FEBS letters

authors： Herrmann JL,Delahay R,Gallagher A,Robertson B,Young D

更新日期：2000-05-19 00:00:00

abstract：:The herpesvirus life cycle has two distinct phases: latency and lytic replication. The viral immediate early protein replication and transcription activator (RTA) plays a central role in mediating the balance between these two phases. Here, we demonstrate that a B cell terminal differentiation factor X-box binding pro...

journal_title：FEBS letters

authors： Yu F,Feng J,Harada JN,Chanda SK,Kenney SC,Sun R

更新日期：2007-07-24 00:00:00

abstract：:C26, the C-terminal 26 residue peptide of serpin A1, significantly increased cell proliferation in cultures of hepatoma cells, but not in porcine kidney epithelial cells, human skin fibroblasts or keratinocytes. The mitogenic activity of C26 was preferentially inhibited with a protein kinase C (PKC) inhibitor, an anti...

journal_title：FEBS letters

authors： Congote LF,Temmel N

更新日期：2004-10-22 00:00:00

abstract：:Neutrophils contain four uncharacterized protein kinases with molecular masses of ca. 69, 63, 49 and 40 kDa that are rapidly activated upon stimulation of these cells with the chemoattractant fMet-Leu-Phe [Ding, J. and Badwey, J.A. (1993) J. Biol. Chem. 268, 17326-17333]. We now report that wortmannin and 1-butanol bl...

journal_title：FEBS letters

authors： Ding J,Badwey JA

更新日期：1994-07-11 00:00:00

abstract：:In bacterial polypeptide synthesis aminoacyl-tRNA (aa-tRNA) bound to elongation factor Tu (EF-Tu) and GTP is part of a crucial intermediate ribonucleoprotein complex involved in the decoding of messenger RNA. The conformation and topology as well as the affinity of the macromolecules in this ternary aa-tRNA X EF-Tu X ...

journal_title：FEBS letters

authors： Faulhammer HG,Joshi RL

更新日期：1987-06-15 00:00:00

abstract：:Inositol 1,4,5-trisphosphate (1,4,5-InsP3) was perfused into rat dorsal root ganglion (DRG) neurons by whole-cell patch-clamp electrodes, while measuring the membrane potential. This operation evoked a transient (2-3 min) membrane hyperpolarization of about -15 mV (from -42 mV) followed by a depolarization. The membra...

journal_title：FEBS letters

authors： Wang Z,Ypey DL,Van Duijn B

更新日期：1992-06-15 00:00:00