Abstract:
:OmpC, a surface antigen of Salmonella typhi was crystallized after several attempts, using PEG 3350. Well shaped hexagonal crystals were grown from vapor diffusion method using octyl glucoside and C12E9 as detergents. Crystals are sensitive to X-ray and diffract weakly up to 7 A. Porin isoforms, due to the bound lipopolysaccharides, could be the cause for poor diffraction. Crystal quality depends largely on the purification method, and in case of LPS contamination, the genetic background of the bacteria. Crystallization and initial data collection suggest optimum conditions and the method of choice for OmpC crystallization.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Arockiasamy A,Krishnaswamy Sdoi
10.1016/s0014-5793(99)00746-2keywords:
subject
Has Abstractpub_date
1999-06-25 00:00:00pages
380-2issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00746-2journal_volume
453pub_type
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