Abstract:
:The mechanisms involved in bidirectional transport along microtubules remain largely unknown. We explored the collective action of kinesin-2 and dynein motors during transport of melanosomes in Xenopus laevis melanophores. These motors are attached to organelles through accessory proteins establishing a complex molecular linker. We determined both the stiffness of this linker and the organelles speed and observed that these parameters depended on the organelle size and cargo direction. Our results suggest that melanosome transport is driven by two dissimilar teams: whereas dynein motors compete with kinesin-2 affecting the properties of plus-end directed organelles, kinesin-2 does not seem to play a similar role during minus-end transport.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
De Rossi MC,De Rossi ME,Sued M,Rodríguez D,Bruno L,Levi Vdoi
10.1016/j.febslet.2015.07.038subject
Has Abstractpub_date
2015-09-14 00:00:00pages
2763-8issue
19 Pt Beissn
0014-5793issn
1873-3468pii
S0014-5793(15)00663-8journal_volume
589pub_type
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