SRC transcriptional activation in a subset of human colon cancer cell lines.

Abstract:

:Human SRC encodes the non-receptor tyrosine kinase pp60(c-Src), which is activated in many human colon cancer cell lines (HCCLs) and tumors. We found that both c-Src protein and mRNA levels were elevated in a subset of HCCLs. Increased c-Src mRNA and protein levels correlated strongly with increased c-Src kinase activity. Nuclear run-on analysis and c-Src mRNA half-life determination demonstrated increased mRNA levels were due to increased transcription of the SRC gene. We also observed decreased c-Src mRNA stability in cell lines that displayed SRC transcriptional activation. Our findings provide the first evidence that SRC transcriptional activation is an important determinant of c-Src expression and activity in HCCLs.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Dehm S,Senger MA,Bonham K

doi

10.1016/s0014-5793(00)02354-1

keywords:

subject

Has Abstract

pub_date

2001-01-05 00:00:00

pages

367-71

issue

3

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(00)02354-1

journal_volume

487

pub_type

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