Abstract:
:Human SRC encodes the non-receptor tyrosine kinase pp60(c-Src), which is activated in many human colon cancer cell lines (HCCLs) and tumors. We found that both c-Src protein and mRNA levels were elevated in a subset of HCCLs. Increased c-Src mRNA and protein levels correlated strongly with increased c-Src kinase activity. Nuclear run-on analysis and c-Src mRNA half-life determination demonstrated increased mRNA levels were due to increased transcription of the SRC gene. We also observed decreased c-Src mRNA stability in cell lines that displayed SRC transcriptional activation. Our findings provide the first evidence that SRC transcriptional activation is an important determinant of c-Src expression and activity in HCCLs.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Dehm S,Senger MA,Bonham Kdoi
10.1016/s0014-5793(00)02354-1keywords:
subject
Has Abstractpub_date
2001-01-05 00:00:00pages
367-71issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(00)02354-1journal_volume
487pub_type
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